Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel

Hydroxyapatite (HA) and collagen have been coated on metallic implants to accelerate osseointegration. Most methods to coat HA require high sintering temperature, high cost and high-energy power while the methods to coat collagen commonly produce unstable coating. Therefore, in this study, a polydop...

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Main Author:	Tapsir, Zafirah
Format:	Thesis
Language:	English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/79091/1/ZafirahTapsirMFBME2018.pdf
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spelling	my-utm-ep.790912018-09-27T06:07:33Z Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel 2018-01 Tapsir, Zafirah TP Chemical technology Hydroxyapatite (HA) and collagen have been coated on metallic implants to accelerate osseointegration. Most methods to coat HA require high sintering temperature, high cost and high-energy power while the methods to coat collagen commonly produce unstable coating. Therefore, in this study, a polydopamine film was used as an intermediate layer to immobilise HA and collagen type I on a medical grade stainless steel (SS316L) due to its versatile, strong and stable properties. The SS316L disks were pre-treated and grafted with a polydopamine film. Then, they were covalently immobilised with collagen fibers at different immersion time (6, 12 and 24 hours). The disks were further biomineralised with HA in 1.5× simulated body fluid (SBF) solution for 7 days. The coated surfaces were characterised using FTIR, FESEM-EDX, XRD and contact angle analyses to investigate its chemical composition, morphology, crystallinity and wettability properties. The characterisation analyses showed that increased in collagen immersion time have induced the formation of amide cross-linkage between collagen and polydopamine. Longer immersion time has also produced less agglomerated carbonate HA with a nano lath-like surface. The disks with longest collagen immersion time were selected and subjected to in vitro test with human fetal osteoblasts (hFOB). The cell attachment, viability and differentiation were examined through FESEM, Alamar Blue reduction assay and Alkaline Phosphatase (ALP) assay respectively. The disks immobilised with HA and collagen presented highest proportion of cell adhesion, highest viability percentage with 31.8% of reduction potential and highest production level of ALP activity at 6.4 μIU/L compared to the bare SS316L disks and the disks immobilised with collagen. It can be concluded that, the polydopamine film has acted as an intermediate layer for the immobilisation of bioactive HA and collagen which projects a promising technique in the development of bioactive implant coating. 2018-01 Thesis http://eprints.utm.my/id/eprint/79091/ http://eprints.utm.my/id/eprint/79091/1/ZafirahTapsirMFBME2018.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:110888 phd doctoral Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Tapsir, Zafirah Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel
description	Hydroxyapatite (HA) and collagen have been coated on metallic implants to accelerate osseointegration. Most methods to coat HA require high sintering temperature, high cost and high-energy power while the methods to coat collagen commonly produce unstable coating. Therefore, in this study, a polydopamine film was used as an intermediate layer to immobilise HA and collagen type I on a medical grade stainless steel (SS316L) due to its versatile, strong and stable properties. The SS316L disks were pre-treated and grafted with a polydopamine film. Then, they were covalently immobilised with collagen fibers at different immersion time (6, 12 and 24 hours). The disks were further biomineralised with HA in 1.5× simulated body fluid (SBF) solution for 7 days. The coated surfaces were characterised using FTIR, FESEM-EDX, XRD and contact angle analyses to investigate its chemical composition, morphology, crystallinity and wettability properties. The characterisation analyses showed that increased in collagen immersion time have induced the formation of amide cross-linkage between collagen and polydopamine. Longer immersion time has also produced less agglomerated carbonate HA with a nano lath-like surface. The disks with longest collagen immersion time were selected and subjected to in vitro test with human fetal osteoblasts (hFOB). The cell attachment, viability and differentiation were examined through FESEM, Alamar Blue reduction assay and Alkaline Phosphatase (ALP) assay respectively. The disks immobilised with HA and collagen presented highest proportion of cell adhesion, highest viability percentage with 31.8% of reduction potential and highest production level of ALP activity at 6.4 μIU/L compared to the bare SS316L disks and the disks immobilised with collagen. It can be concluded that, the polydopamine film has acted as an intermediate layer for the immobilisation of bioactive HA and collagen which projects a promising technique in the development of bioactive implant coating.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Tapsir, Zafirah
author_facet	Tapsir, Zafirah
author_sort	Tapsir, Zafirah
title	Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel
title_short	Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel
title_full	Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel
title_fullStr	Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel
title_full_unstemmed	Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel
title_sort	incorporation of bioactive collagen type i and biomineralised hydroxyapatite on polydopamine grafted stainless steel
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2018
url	http://eprints.utm.my/id/eprint/79091/1/ZafirahTapsirMFBME2018.pdf
_version_	1747818144584433664

Incorporation of bioactive collagen type I and biomineralised hydroxyapatite on polydopamine grafted stainless steel

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