The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles

Breast cancer is among the major killer diseases for women. Currently chemotherapy is the common method used for such cancer treatment. The drawback with this technique is that it kills both normal and cancerous cells. As a result not many cancer patients can survive after the treatment. Thus, alter...

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Main Author:	Badruzzaman, Aishah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QC Physics
Online Access:	http://eprints.utm.my/id/eprint/79225/1/AishahBadruzzamanPFS2017.pdf
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spelling	my-utm-ep.792252018-10-14T08:39:39Z The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles 2017 Badruzzaman, Aishah QC Physics Breast cancer is among the major killer diseases for women. Currently chemotherapy is the common method used for such cancer treatment. The drawback with this technique is that it kills both normal and cancerous cells. As a result not many cancer patients can survive after the treatment. Thus, alternative techniques need to be considered. The aim of this project is to introduce laser radiation instead of x-ray for cancer treatment. In order to further enhance the hyperthermia effect, gold nanoparticles (AuNP) are injected to couple with cancerous cell. In this work, laser irradiation with three different wavelengths were used for the treatment namely, 1064 nm, and 532 nm produced by Q-switched Nd:YAG laser and 248 nm produced by Krypton Fluoride Excimer laser. AuNPs were produced using pulse laser ablation in liquid technique. The AuNP diameter was varied in the range of 8-18 nm and concentration was prepared by serial dilution within the range of 0.14-4.50 μg/ml. Three different human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MDA-kb2) and a Chinese Hamster Ovary (CHO) non-cancerous cell were used to study the cell survivability towards hyperthermia effect with and without the presence of AuNPs. The cell survivability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and analyzed using GloMax Multi Microplate Multimode Reader. The results show that survivability rate of MDA-MB-231 cell was 84 percent after treated with 1064 nm laser compared to 74 percent after treated with 532 nm laser. UV radiation from Excimer laser is found to increase the cell survivability rate up to 157 percent. The survivability rate of CHO cell treated with 532 nm laser dropped by 9 percent compared to those of MDA-MB-231, MDAMB- 468 and MDA-kb2 cells which decreased by more than 20 percent after the treatment. The total number of survival cell decreases with the presence of AuNPs. The results indicate that higher concentration and smaller size of AuNPs minimized the MDA-MB-231 breast cancer cell survivability. Therefore, hyperthermia effect can be enhanced by using laser irradiation sensitized with AuNP and has great potential for treating cancer. 2017 Thesis http://eprints.utm.my/id/eprint/79225/ http://eprints.utm.my/id/eprint/79225/1/AishahBadruzzamanPFS2017.pdf application/pdf en public phd doctoral Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QC Physics
spellingShingle	QC Physics Badruzzaman, Aishah The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
description	Breast cancer is among the major killer diseases for women. Currently chemotherapy is the common method used for such cancer treatment. The drawback with this technique is that it kills both normal and cancerous cells. As a result not many cancer patients can survive after the treatment. Thus, alternative techniques need to be considered. The aim of this project is to introduce laser radiation instead of x-ray for cancer treatment. In order to further enhance the hyperthermia effect, gold nanoparticles (AuNP) are injected to couple with cancerous cell. In this work, laser irradiation with three different wavelengths were used for the treatment namely, 1064 nm, and 532 nm produced by Q-switched Nd:YAG laser and 248 nm produced by Krypton Fluoride Excimer laser. AuNPs were produced using pulse laser ablation in liquid technique. The AuNP diameter was varied in the range of 8-18 nm and concentration was prepared by serial dilution within the range of 0.14-4.50 μg/ml. Three different human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MDA-kb2) and a Chinese Hamster Ovary (CHO) non-cancerous cell were used to study the cell survivability towards hyperthermia effect with and without the presence of AuNPs. The cell survivability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and analyzed using GloMax Multi Microplate Multimode Reader. The results show that survivability rate of MDA-MB-231 cell was 84 percent after treated with 1064 nm laser compared to 74 percent after treated with 532 nm laser. UV radiation from Excimer laser is found to increase the cell survivability rate up to 157 percent. The survivability rate of CHO cell treated with 532 nm laser dropped by 9 percent compared to those of MDA-MB-231, MDAMB- 468 and MDA-kb2 cells which decreased by more than 20 percent after the treatment. The total number of survival cell decreases with the presence of AuNPs. The results indicate that higher concentration and smaller size of AuNPs minimized the MDA-MB-231 breast cancer cell survivability. Therefore, hyperthermia effect can be enhanced by using laser irradiation sensitized with AuNP and has great potential for treating cancer.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Badruzzaman, Aishah
author_facet	Badruzzaman, Aishah
author_sort	Badruzzaman, Aishah
title	The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
title_short	The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
title_full	The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
title_fullStr	The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
title_full_unstemmed	The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
title_sort	effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2017
url	http://eprints.utm.my/id/eprint/79225/1/AishahBadruzzamanPFS2017.pdf
_version_	1747818176627867648

The effect of laser irradiation on survivability of breast canser cells sensitised with gold nanoparticles

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