Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture
Demand for cell culture platform has drawn increasing attention due to their ease for cell-based drug screening. Typically, cell culture platforms are made from non-biodegradable materials such as polystyrene or polyethylene. With respect to in vitro applications; we proposed an alternative platform...
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my-utm-ep.794432018-10-21T04:34:49Z Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture 2017 Muhamad @ Wahab, Norsamsiah QH Natural history Demand for cell culture platform has drawn increasing attention due to their ease for cell-based drug screening. Typically, cell culture platforms are made from non-biodegradable materials such as polystyrene or polyethylene. With respect to in vitro applications; we proposed an alternative platform from wax-impregnated cotton fabrics to promote cells proliferation. The techniques, which consisted of cutting,dipping, folding and drying were conducted without any high-end and costly equipment. The chemical compositions of the substrate (wax) such as hydroxyl(OH-) and carbonyl groups (C=O, C-O) were attributed to hydrophilic property based on the effect of protein adsorptions. The water contact angle on the wax formulations was in a range of 80° to 100°. Due to different melting point of each wax, which is in a range of 53°C – 86°C). Several factors such as melting point, weight ratios of wax, and viscosity affected the surface roughness of the samples. Hence, hierarchical micro and nano roughness were found on wax-impregnated cotton fabrics surfaces which facilitated different actin cytoskeleton. Sample 1 (S1) has higher hydrophobicity, lower surface roughness and lowest stickiness where actin cytoskeletons were observed to spread uniformly compared to sample 2 (S2) which has lower hydrophobicity, highest surface roughness and moderate stickiness wherethe actin cytoskeletons were bound to the nucleus cells. Sample 3 (S3) and sample 4(S4) have quite similar cell morphology, sticked and spread. There was no significant difference in biocompatibility for all samples formulations, when compared to commercial 96 well-plate (Thermo scientificTM) but there was a statistical significant differences (student t-test, P<0.01). The healthy and non-healthy cells were stained using trichrome stain for observation under light microscopy. There was no significant difference in proliferations; size of nucleus and cytoplasm for normal and abnormal cells; however cells morphology in the S2 were similar to the morphology in the in-vivo. Further investigation on real tissues from patient is required to test further and apply this newly developed platform for clinical applications. 2017 Thesis http://eprints.utm.my/id/eprint/79443/ phd doctoral Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering |
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Universiti Teknologi Malaysia |
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QH Natural history |
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QH Natural history Muhamad @ Wahab, Norsamsiah Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
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Demand for cell culture platform has drawn increasing attention due to their ease for cell-based drug screening. Typically, cell culture platforms are made from non-biodegradable materials such as polystyrene or polyethylene. With respect to in vitro applications; we proposed an alternative platform from wax-impregnated cotton fabrics to promote cells proliferation. The techniques, which consisted of cutting,dipping, folding and drying were conducted without any high-end and costly equipment. The chemical compositions of the substrate (wax) such as hydroxyl(OH-) and carbonyl groups (C=O, C-O) were attributed to hydrophilic property based on the effect of protein adsorptions. The water contact angle on the wax formulations was in a range of 80° to 100°. Due to different melting point of each wax, which is in a range of 53°C – 86°C). Several factors such as melting point, weight ratios of wax, and viscosity affected the surface roughness of the samples. Hence, hierarchical micro and nano roughness were found on wax-impregnated cotton fabrics surfaces which facilitated different actin cytoskeleton. Sample 1 (S1) has higher hydrophobicity, lower surface roughness and lowest stickiness where actin cytoskeletons were observed to spread uniformly compared to sample 2 (S2) which has lower hydrophobicity, highest surface roughness and moderate stickiness wherethe actin cytoskeletons were bound to the nucleus cells. Sample 3 (S3) and sample 4(S4) have quite similar cell morphology, sticked and spread. There was no significant difference in biocompatibility for all samples formulations, when compared to commercial 96 well-plate (Thermo scientificTM) but there was a statistical significant differences (student t-test, P<0.01). The healthy and non-healthy cells were stained using trichrome stain for observation under light microscopy. There was no significant difference in proliferations; size of nucleus and cytoplasm for normal and abnormal cells; however cells morphology in the S2 were similar to the morphology in the in-vivo. Further investigation on real tissues from patient is required to test further and apply this newly developed platform for clinical applications. |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Muhamad @ Wahab, Norsamsiah |
| author_facet |
Muhamad @ Wahab, Norsamsiah |
| author_sort |
Muhamad @ Wahab, Norsamsiah |
| title |
Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
| title_short |
Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
| title_full |
Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
| title_fullStr |
Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
| title_full_unstemmed |
Hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
| title_sort |
hierarchical surface roughness wax-impregnated cotton fabrics platform for cell culture |
| granting_institution |
Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering |
| granting_department |
Faculty of Biosciences and Medical Engineering |
| publishDate |
2017 |
| _version_ |
1747818228455833600 |