Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark

Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark

Formation of advanced glycation end products (AGEs) under hyperglycemic condition in diabetes mellitus results in micro/macro-angiopathy disorders. Juice, leaves, or peel of pomegranate have shown antioxidant or antiglycation effects. Pomegranate stem barks which are hugely wasted during the pruning...

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Main Author: Khangholi, Shahpour
Format: Thesis
Language:English
Published: 2017
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/79516/1/ShahpourKhangholiPFChE2017.pdf
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spelling my-utm-ep.795162018-10-31T12:52:58Z Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark 2017 Khangholi, Shahpour TP Chemical technology Formation of advanced glycation end products (AGEs) under hyperglycemic condition in diabetes mellitus results in micro/macro-angiopathy disorders. Juice, leaves, or peel of pomegranate have shown antioxidant or antiglycation effects. Pomegranate stem barks which are hugely wasted during the pruning season could be a good source of phyto-based anti-AGEs. This study evaluated standardized pomegranate stem barks extract in term of antioxidant activity, antiglycation potential and also its effect on lipid formation and glucose consumption in 3T3-L1 cells.Various extraction conditions were performed including types of solvents, time and type of extraction methods. Phytochemical analysis of extracts was carried by highperformance liquid chromatography-pulsed amperometric detector (HPLC-PAD), gas chromatography-mass spectroscopy and spectrophotometric methods. Evaluation of antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′- Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and metal chelating activity. Anti glycation activity of the extract was evaluated in bovine serum albumin (BSA)/glucose or BSA/methylglyoxal (MGO) bioassay systems in presence or absence of samples. Antiglycation property was measured by determination of the level of formation of fructosamine, protein carbonyl and AGE or loss of thiol group. Also, effect of extract on glucose consumption and lipid formation in 3T3-L1 cell line in media containing MGO was investigated in vitro. The result showed that eight hour extraction with methanol using Soxhlet extraction (SM8) was the best extraction process in term of total polyphenolic compounds (59.69 ± 2.913 mg gallic acid equivalent (GAE)/g dry weight (DW), DPPH scavenging capacity [half maximal effective concentration (EC50) 14.99 ± 1.18 mg/L], ABTS●+ radical scavenging equal to 2.636 mM trolox equivalent antioxidant capacity (TEAC)/100 g DW and metal chelation activity (EC50 888.1±48.38). Standardization of SM8 extract by HPLC-PAD showed gallic acid as 0.19% and catechin 0.03% of the extract. SM8 extract reduced formation of AGE significantly (p<0.01) by 77% in concentration of 250 μg /ml. Moreover, it reduced protein carbonyl (60.2%) and fructosamine formation (33.99 %) and simultaneously inhibited thiol group loss (by 1.84 folds). The SM8 extract increased glucose consumption (by 1.95 folds) in 3T3-L1 cells in glycemic condition. In conclusion, it is recommended that pomegranate stem bark extract as a potential source of raw material to be further investigated for the development of health supplement with AGEs inhibitory properties. 2017 Thesis http://eprints.utm.my/id/eprint/79516/ http://eprints.utm.my/id/eprint/79516/1/ShahpourKhangholiPFChE2017.pdf application/pdf en public phd doctoral Universiti Teknologi Malaysia, Faculty of Chemical & Energy Engineering Faculty of Chemical & Energy Engineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Khangholi, Shahpour
Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark
description Formation of advanced glycation end products (AGEs) under hyperglycemic condition in diabetes mellitus results in micro/macro-angiopathy disorders. Juice, leaves, or peel of pomegranate have shown antioxidant or antiglycation effects. Pomegranate stem barks which are hugely wasted during the pruning season could be a good source of phyto-based anti-AGEs. This study evaluated standardized pomegranate stem barks extract in term of antioxidant activity, antiglycation potential and also its effect on lipid formation and glucose consumption in 3T3-L1 cells.Various extraction conditions were performed including types of solvents, time and type of extraction methods. Phytochemical analysis of extracts was carried by highperformance liquid chromatography-pulsed amperometric detector (HPLC-PAD), gas chromatography-mass spectroscopy and spectrophotometric methods. Evaluation of antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′- Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and metal chelating activity. Anti glycation activity of the extract was evaluated in bovine serum albumin (BSA)/glucose or BSA/methylglyoxal (MGO) bioassay systems in presence or absence of samples. Antiglycation property was measured by determination of the level of formation of fructosamine, protein carbonyl and AGE or loss of thiol group. Also, effect of extract on glucose consumption and lipid formation in 3T3-L1 cell line in media containing MGO was investigated in vitro. The result showed that eight hour extraction with methanol using Soxhlet extraction (SM8) was the best extraction process in term of total polyphenolic compounds (59.69 ± 2.913 mg gallic acid equivalent (GAE)/g dry weight (DW), DPPH scavenging capacity [half maximal effective concentration (EC50) 14.99 ± 1.18 mg/L], ABTS●+ radical scavenging equal to 2.636 mM trolox equivalent antioxidant capacity (TEAC)/100 g DW and metal chelation activity (EC50 888.1±48.38). Standardization of SM8 extract by HPLC-PAD showed gallic acid as 0.19% and catechin 0.03% of the extract. SM8 extract reduced formation of AGE significantly (p<0.01) by 77% in concentration of 250 μg /ml. Moreover, it reduced protein carbonyl (60.2%) and fructosamine formation (33.99 %) and simultaneously inhibited thiol group loss (by 1.84 folds). The SM8 extract increased glucose consumption (by 1.95 folds) in 3T3-L1 cells in glycemic condition. In conclusion, it is recommended that pomegranate stem bark extract as a potential source of raw material to be further investigated for the development of health supplement with AGEs inhibitory properties.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Khangholi, Shahpour
author_facet Khangholi, Shahpour
author_sort Khangholi, Shahpour
title Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark
title_short Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark
title_full Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark
title_fullStr Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark
title_full_unstemmed Advanced glycation end products formation inhibition through standardized crude extract of punica granatum L. stem bark
title_sort advanced glycation end products formation inhibition through standardized crude extract of punica granatum l. stem bark
granting_institution Universiti Teknologi Malaysia, Faculty of Chemical & Energy Engineering
granting_department Faculty of Chemical & Energy Engineering
publishDate 2017
url http://eprints.utm.my/id/eprint/79516/1/ShahpourKhangholiPFChE2017.pdf
_version_ 1747818245396627456

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