Starch degrading bacteria for biohydrogen production

Global research is moving forward in developing biological production of hydrogen as a renewable energy source in order to reduce the use of non-renewable energy. Biohydrogen has the potential of replacing the hydrogen production by fossil fuels which is one of the major factors that cause the globa...

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Main Author:	Abubakar, Attahiru
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/80906/1/AttahiruAbuBakarMFBME2016.pdf
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id	my-utm-ep.80906
record_format	uketd_dc
spelling	my-utm-ep.809062019-07-24T00:10:33Z Starch degrading bacteria for biohydrogen production 2016-01 Abubakar, Attahiru TP Chemical technology Global research is moving forward in developing biological production of hydrogen as a renewable energy source in order to reduce the use of non-renewable energy. Biohydrogen has the potential of replacing the hydrogen production by fossil fuels which is one of the major factors that cause the global warming. The amylolytic activity of several microorganisms capable of utilizing starch in the dark fermentation process has provided a great potential in biohydrogen production by the starch degrading bacteria. In this study, starch utilizing bacteria have been successfully isolated from cassava chips processing wastewater and sludge. Based on the morphological characteristics, the isolate was found to be Gram positive bacteria with spherical cell shape. 16S rRNA analysis identified the strain Lactococcus sp. SDB4 with 92% similarity to Lactococcus lactis. The partial 16S rRNA sequence has been deposited to GenBank with Accession Number KU160544. Furthermore, the isolate was subjected to anaerobic dark fermentation process using synthetic media with tapioca starch as the only source of carbon. The findings in this study indicate that the Lactococcus sp. SDB4 possesses an amylolytic activity and capable of utilizing starch efficiently (75%). This strain has the potential to be used in biohydrogen production using starch containing wastewater as substrate. 2016-01 Thesis http://eprints.utm.my/id/eprint/80906/ http://eprints.utm.my/id/eprint/80906/1/AttahiruAbuBakarMFBME2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:120511 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abubakar, Attahiru Starch degrading bacteria for biohydrogen production
description	Global research is moving forward in developing biological production of hydrogen as a renewable energy source in order to reduce the use of non-renewable energy. Biohydrogen has the potential of replacing the hydrogen production by fossil fuels which is one of the major factors that cause the global warming. The amylolytic activity of several microorganisms capable of utilizing starch in the dark fermentation process has provided a great potential in biohydrogen production by the starch degrading bacteria. In this study, starch utilizing bacteria have been successfully isolated from cassava chips processing wastewater and sludge. Based on the morphological characteristics, the isolate was found to be Gram positive bacteria with spherical cell shape. 16S rRNA analysis identified the strain Lactococcus sp. SDB4 with 92% similarity to Lactococcus lactis. The partial 16S rRNA sequence has been deposited to GenBank with Accession Number KU160544. Furthermore, the isolate was subjected to anaerobic dark fermentation process using synthetic media with tapioca starch as the only source of carbon. The findings in this study indicate that the Lactococcus sp. SDB4 possesses an amylolytic activity and capable of utilizing starch efficiently (75%). This strain has the potential to be used in biohydrogen production using starch containing wastewater as substrate.
format	Thesis
qualification_level	Master's degree
author	Abubakar, Attahiru
author_facet	Abubakar, Attahiru
author_sort	Abubakar, Attahiru
title	Starch degrading bacteria for biohydrogen production
title_short	Starch degrading bacteria for biohydrogen production
title_full	Starch degrading bacteria for biohydrogen production
title_fullStr	Starch degrading bacteria for biohydrogen production
title_full_unstemmed	Starch degrading bacteria for biohydrogen production
title_sort	starch degrading bacteria for biohydrogen production
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/80906/1/AttahiruAbuBakarMFBME2016.pdf
_version_	1747818278785384448

Starch degrading bacteria for biohydrogen production

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