Starch degradation and biohydrogen generation by Bacillus sp. SSA

Population increase has also increased the need for energy. The use of nonrenewable fossil fuels as the main energy supply has decreased its resources and hydrogen become an attractive substitute for it. Besides, environmental pollution has always accompanied the use of fossil fuels. Hence, simultan...

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Main Author:	Aziz, Fatin Syuhadah
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/80997/1/FatinSyuhadahAzizMFBME2016.pdf
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spelling	my-utm-ep.809972019-07-24T00:13:55Z Starch degradation and biohydrogen generation by Bacillus sp. SSA 2016-10 Aziz, Fatin Syuhadah TP Chemical technology Population increase has also increased the need for energy. The use of nonrenewable fossil fuels as the main energy supply has decreased its resources and hydrogen become an attractive substitute for it. Besides, environmental pollution has always accompanied the use of fossil fuels. Hence, simultaneous treatment of wastes and energy generation is desirable. Starch-containing cassava wastewater has the potential to be used as the feedstock for biohydrogen production via dark fermentation process. In the present study, effective starch utilization and biohydrogen production by Bacillus sp. SSA was investigated. It was observed that Bacillus sp. SSA produced hydrogen as early as 12 hours after batch culture fermentation starts. In order to find the optimum conditions for biohydrogen production, batch experiments were conducted at various temperature (30-40ºC), initial medium pH (4.5-5.5) and initial substrate concentrations (0.5-1.5 g/L). The maximum biohydrogen production was obtained at temperature 30ºC, pH 5.5 and initial substrate concentration 1.0 g/L with a total value of 0.141 mL. The biohydrogen production rate, starch utilization, specific growth rate, maximum specific growth rate and yield of product on cell at this condition were the highest with a total of 0.008 mL/h, 23.9%, 0.013 h-1, 0.026 h-1 and 2.104 mL/g, respectively. This study supports the potential of simultaneous cassava wastewater treatment and biohydrogen production using Bacillus sp. SSA strain as the biological agent. 2016-10 Thesis http://eprints.utm.my/id/eprint/80997/ http://eprints.utm.my/id/eprint/80997/1/FatinSyuhadahAzizMFBME2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:120513 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Aziz, Fatin Syuhadah Starch degradation and biohydrogen generation by Bacillus sp. SSA
description	Population increase has also increased the need for energy. The use of nonrenewable fossil fuels as the main energy supply has decreased its resources and hydrogen become an attractive substitute for it. Besides, environmental pollution has always accompanied the use of fossil fuels. Hence, simultaneous treatment of wastes and energy generation is desirable. Starch-containing cassava wastewater has the potential to be used as the feedstock for biohydrogen production via dark fermentation process. In the present study, effective starch utilization and biohydrogen production by Bacillus sp. SSA was investigated. It was observed that Bacillus sp. SSA produced hydrogen as early as 12 hours after batch culture fermentation starts. In order to find the optimum conditions for biohydrogen production, batch experiments were conducted at various temperature (30-40ºC), initial medium pH (4.5-5.5) and initial substrate concentrations (0.5-1.5 g/L). The maximum biohydrogen production was obtained at temperature 30ºC, pH 5.5 and initial substrate concentration 1.0 g/L with a total value of 0.141 mL. The biohydrogen production rate, starch utilization, specific growth rate, maximum specific growth rate and yield of product on cell at this condition were the highest with a total of 0.008 mL/h, 23.9%, 0.013 h-1, 0.026 h-1 and 2.104 mL/g, respectively. This study supports the potential of simultaneous cassava wastewater treatment and biohydrogen production using Bacillus sp. SSA strain as the biological agent.
format	Thesis
qualification_level	Master's degree
author	Aziz, Fatin Syuhadah
author_facet	Aziz, Fatin Syuhadah
author_sort	Aziz, Fatin Syuhadah
title	Starch degradation and biohydrogen generation by Bacillus sp. SSA
title_short	Starch degradation and biohydrogen generation by Bacillus sp. SSA
title_full	Starch degradation and biohydrogen generation by Bacillus sp. SSA
title_fullStr	Starch degradation and biohydrogen generation by Bacillus sp. SSA
title_full_unstemmed	Starch degradation and biohydrogen generation by Bacillus sp. SSA
title_sort	starch degradation and biohydrogen generation by bacillus sp. ssa
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/80997/1/FatinSyuhadahAzizMFBME2016.pdf
_version_	1747818289425285120

Starch degradation and biohydrogen generation by Bacillus sp. SSA

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