Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria

An increase in the number of antibiotic resistant bacteria worldwide has necessitated new antibacterial agents, mainly by medicinal plants. For this, the antioxidant and antibacterial activities of four Malaysian medicinal plants; Alpinia galanga, Centella asiatica, Clinacanthus nutans and Persicari...

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Main Author:	Zakaria, Intan Nursuraya
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/81034/1/IntanNursurayaZakariaMFS2019.pdf
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id	my-utm-ep.81034
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spelling	my-utm-ep.810342019-07-24T03:06:13Z Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria 2019-03 Zakaria, Intan Nursuraya R Medicine (General) An increase in the number of antibiotic resistant bacteria worldwide has necessitated new antibacterial agents, mainly by medicinal plants. For this, the antioxidant and antibacterial activities of four Malaysian medicinal plants; Alpinia galanga, Centella asiatica, Clinacanthus nutans and Persicaria odorata extracts were evaluated against gram-positive (Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA)) and gram-negative (Escherichia coli, Pseudomonas putida) in this study. The medicinal plants were extracted using solvents with different polarities and screened for the total phenolic contents via Folin-Ciocalteu method and antioxidant capacity by diphenyl-1-picrylhydrazyl (DPPH) activity. The antibacterial activities were conducted using disc diffusion, minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. The most effective plant extract was further fractionated by column chromatography and thin layer chromatography. The fractions were then characterized using antibacterial assays, Zeta potential measurements, followed by scanning electron microscopic (SEM) observations and Gas Chromatography Mass Spectrometry (GCMS) analysis. Compounds identified were docked with Penicillin binding protein (PBP) using Autodock 4.0 and simulate (MD) by Gromacs 5.0.4. The findings from this study showed that acetone plant extracts exhibited the highest antioxidant and antibacterial activities; significantly by P. odorata acetone extract. This extract was also chosen due to its comparable MIC and MBC values with both the positive controls respectively. A total of 12 fractions were separated from the extract, however only H05 fraction showed bactericidal action against all gram-positive bacteria. This fraction could also alter the magnitude of bacterial negativity, significantly against MRSA. This data was supported by morphological alterations induced in treated MRSA, through SEM images. The GCMS analysis of H05 fraction showed that the presence of seven major phenolic compounds derivatives that met the Lipinski’s Rule of Five were present in the H05 extract. Among the seven compounds, the MD trajectory analysis showed that 6- hydroxy-8-methoxyoctanoic acid (tannins derivatives) shows strongest and most stable binding with PBP protein. The present study indicates that phenolics mainly tannins present in the P. odorata acetone extract were highly responsible for its antibacterial potential, especially for MRSA infection treatment. 2019-03 Thesis http://eprints.utm.my/id/eprint/81034/ http://eprints.utm.my/id/eprint/81034/1/IntanNursurayaZakariaMFS2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:124995 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Zakaria, Intan Nursuraya Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
description	An increase in the number of antibiotic resistant bacteria worldwide has necessitated new antibacterial agents, mainly by medicinal plants. For this, the antioxidant and antibacterial activities of four Malaysian medicinal plants; Alpinia galanga, Centella asiatica, Clinacanthus nutans and Persicaria odorata extracts were evaluated against gram-positive (Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA)) and gram-negative (Escherichia coli, Pseudomonas putida) in this study. The medicinal plants were extracted using solvents with different polarities and screened for the total phenolic contents via Folin-Ciocalteu method and antioxidant capacity by diphenyl-1-picrylhydrazyl (DPPH) activity. The antibacterial activities were conducted using disc diffusion, minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. The most effective plant extract was further fractionated by column chromatography and thin layer chromatography. The fractions were then characterized using antibacterial assays, Zeta potential measurements, followed by scanning electron microscopic (SEM) observations and Gas Chromatography Mass Spectrometry (GCMS) analysis. Compounds identified were docked with Penicillin binding protein (PBP) using Autodock 4.0 and simulate (MD) by Gromacs 5.0.4. The findings from this study showed that acetone plant extracts exhibited the highest antioxidant and antibacterial activities; significantly by P. odorata acetone extract. This extract was also chosen due to its comparable MIC and MBC values with both the positive controls respectively. A total of 12 fractions were separated from the extract, however only H05 fraction showed bactericidal action against all gram-positive bacteria. This fraction could also alter the magnitude of bacterial negativity, significantly against MRSA. This data was supported by morphological alterations induced in treated MRSA, through SEM images. The GCMS analysis of H05 fraction showed that the presence of seven major phenolic compounds derivatives that met the Lipinski’s Rule of Five were present in the H05 extract. Among the seven compounds, the MD trajectory analysis showed that 6- hydroxy-8-methoxyoctanoic acid (tannins derivatives) shows strongest and most stable binding with PBP protein. The present study indicates that phenolics mainly tannins present in the P. odorata acetone extract were highly responsible for its antibacterial potential, especially for MRSA infection treatment.
format	Thesis
qualification_level	Master's degree
author	Zakaria, Intan Nursuraya
author_facet	Zakaria, Intan Nursuraya
author_sort	Zakaria, Intan Nursuraya
title	Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
title_short	Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
title_full	Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
title_fullStr	Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
title_full_unstemmed	Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
title_sort	antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2019
url	http://eprints.utm.my/id/eprint/81034/1/IntanNursurayaZakariaMFS2019.pdf
_version_	1747818296862834688

Antioxidant, antibacterial activity and in silico study of selected medicinal plants against pathogenic bacteria

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