Characterization of xylanase from microbulbifer sp. CL37 for industrial applications

Xylan is the most abundant sugar in hemicellulose and can be found in plant biomass. Xylanase produced by microorganisms such as bacteria can be used in industries such as paper and pulp for deinking process. A halophilic bacterium, Microbulbifer sp. strain CL37, was previously isolated from mangrov...

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Main Author:	Mah, Ming Hui
Format:	Thesis
Language:	English
Published:	2019
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/81093/1/MahMingHuiMFS2019.pdf
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spelling	my-utm-ep.810932019-07-24T03:09:35Z Characterization of xylanase from microbulbifer sp. CL37 for industrial applications 2019-01 Mah, Ming Hui Q Science (General) Xylan is the most abundant sugar in hemicellulose and can be found in plant biomass. Xylanase produced by microorganisms such as bacteria can be used in industries such as paper and pulp for deinking process. A halophilic bacterium, Microbulbifer sp. strain CL37, was previously isolated from mangrove sediment and its extracellular xylanase was characterized in this study. Strain CL37 is a motile Gram-negative bacterium with rod shape, catalase, and oxidase positive. Strain CL37 also can hydrolyse xylan, casein, gelatin, Tween 20, Tween 40, Tween 60 and Tween 80. Cells are sensitive to gentamicin, tetracycline, polymyxin B, doxycycline, minocycline and rifampicin. The xylanase exhibited maximum activity at 70°C, pH7, and absent of NaCl. The xylanase remained activity up to 14% (w/v) NaCl indicates it is halotolerant xylanase. The xylanase activity was enhanced in the presence of Al3+, Ca2+, Co2+, Cu+, Cu2+, Fe2+, Fe3+, Mn2+, and Zn2+ (112-175% relative activity), stable in K+, Na+, and Ni2+ (>80% relative activity), but reduced in the presence of Mg2+ (59% relative activity). The xylanase activity also enhanced in the presence of acetone (127% relative activity) and remains stable (>70% relative activity) in most of the tested detergent constituents. Xylanase is also compatible with commercial detergents such as Top®, Dynamo®, Sunlight®, Glo®, Breeze® and Dixan®. Evaluation of the enzymatic deinking activity demonstrated that xylanase from strain CL37 has the ability to detach the adsorbed ink particle from the surface of paper. Collectively, xylanase from Microbulbifer sp. strain CL37 could have potential in various applications, such as detergent formulation, lignocellulolytic biofuel production and paper deinking. 2019-01 Thesis http://eprints.utm.my/id/eprint/81093/ http://eprints.utm.my/id/eprint/81093/1/MahMingHuiMFS2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:125194 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mah, Ming Hui Characterization of xylanase from microbulbifer sp. CL37 for industrial applications
description	Xylan is the most abundant sugar in hemicellulose and can be found in plant biomass. Xylanase produced by microorganisms such as bacteria can be used in industries such as paper and pulp for deinking process. A halophilic bacterium, Microbulbifer sp. strain CL37, was previously isolated from mangrove sediment and its extracellular xylanase was characterized in this study. Strain CL37 is a motile Gram-negative bacterium with rod shape, catalase, and oxidase positive. Strain CL37 also can hydrolyse xylan, casein, gelatin, Tween 20, Tween 40, Tween 60 and Tween 80. Cells are sensitive to gentamicin, tetracycline, polymyxin B, doxycycline, minocycline and rifampicin. The xylanase exhibited maximum activity at 70°C, pH7, and absent of NaCl. The xylanase remained activity up to 14% (w/v) NaCl indicates it is halotolerant xylanase. The xylanase activity was enhanced in the presence of Al3+, Ca2+, Co2+, Cu+, Cu2+, Fe2+, Fe3+, Mn2+, and Zn2+ (112-175% relative activity), stable in K+, Na+, and Ni2+ (>80% relative activity), but reduced in the presence of Mg2+ (59% relative activity). The xylanase activity also enhanced in the presence of acetone (127% relative activity) and remains stable (>70% relative activity) in most of the tested detergent constituents. Xylanase is also compatible with commercial detergents such as Top®, Dynamo®, Sunlight®, Glo®, Breeze® and Dixan®. Evaluation of the enzymatic deinking activity demonstrated that xylanase from strain CL37 has the ability to detach the adsorbed ink particle from the surface of paper. Collectively, xylanase from Microbulbifer sp. strain CL37 could have potential in various applications, such as detergent formulation, lignocellulolytic biofuel production and paper deinking.
format	Thesis
qualification_level	Master's degree
author	Mah, Ming Hui
author_facet	Mah, Ming Hui
author_sort	Mah, Ming Hui
title	Characterization of xylanase from microbulbifer sp. CL37 for industrial applications
title_short	Characterization of xylanase from microbulbifer sp. CL37 for industrial applications
title_full	Characterization of xylanase from microbulbifer sp. CL37 for industrial applications
title_fullStr	Characterization of xylanase from microbulbifer sp. CL37 for industrial applications
title_full_unstemmed	Characterization of xylanase from microbulbifer sp. CL37 for industrial applications
title_sort	characterization of xylanase from microbulbifer sp. cl37 for industrial applications
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2019
url	http://eprints.utm.my/id/eprint/81093/1/MahMingHuiMFS2019.pdf
_version_	1747818305209499648

Characterization of xylanase from microbulbifer sp. CL37 for industrial applications

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