Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system

A new multi-stacking pre-concentration procedure based on field-enhanced sample injection (FESI), field-amplified sample stacking, and transient isotachophoresis was developed. The new procedure was implemented in a compact microchip electrophoresis (MCE) with a double T-junction glass chip, coupled...

Full description

Saved in:

Bibliographic Details
Main Author:	Tai, Chee Tung
Format:	Thesis
Language:	English
Published:	2018
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/81387/1/TaiCheeTungMFS2019.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.81387
record_format	uketd_dc
spelling	my-utm-ep.813872019-08-23T04:07:00Z Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system 2018 Tai, Chee Tung QD Chemistry A new multi-stacking pre-concentration procedure based on field-enhanced sample injection (FESI), field-amplified sample stacking, and transient isotachophoresis was developed. The new procedure was implemented in a compact microchip electrophoresis (MCE) with a double T-junction glass chip, coupled with an on-chip capacitively coupled contactless conductivity detection (C4D) system. A mixture of the cationic target analyte and the terminating electrolyte (TE) from the two sample reservoirs was injected under FESI conditions within the two sample-loading channels. At the double-T junction, the stacked analyte zones were further concentrated under field-amplified stacking conditions and then subsequently focused by transient-isotachophoresis and separated along the separation channels. The proposed multi-stacking strategy was verified under an Universal Serial Bus (USB) fluorescence microscope employing Rhodamine 6G as the model analyte. This developed approach was subsequently used to monitor the target quinine present in human plasma samples. The total analysis time for quinine was approximately 200 s with a sensitivity enhancement factor of approximately 61 when compared to the typical gated injection. The detection and quantification limits of the developed approach for quinine were 3.0 µg/mL and 10 µg/mL, respectively, with intraday and interday repeatability (%RSDs, n=5) of 3.6 % and 4.4 %. Recoveries in spiked human plasma were 98.1 % – 99.8 %. 2018 Thesis http://eprints.utm.my/id/eprint/81387/ http://eprints.utm.my/id/eprint/81387/1/TaiCheeTungMFS2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:125066 masters Universiti Teknologi Malaysia Chemistry
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Tai, Chee Tung Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
description	A new multi-stacking pre-concentration procedure based on field-enhanced sample injection (FESI), field-amplified sample stacking, and transient isotachophoresis was developed. The new procedure was implemented in a compact microchip electrophoresis (MCE) with a double T-junction glass chip, coupled with an on-chip capacitively coupled contactless conductivity detection (C4D) system. A mixture of the cationic target analyte and the terminating electrolyte (TE) from the two sample reservoirs was injected under FESI conditions within the two sample-loading channels. At the double-T junction, the stacked analyte zones were further concentrated under field-amplified stacking conditions and then subsequently focused by transient-isotachophoresis and separated along the separation channels. The proposed multi-stacking strategy was verified under an Universal Serial Bus (USB) fluorescence microscope employing Rhodamine 6G as the model analyte. This developed approach was subsequently used to monitor the target quinine present in human plasma samples. The total analysis time for quinine was approximately 200 s with a sensitivity enhancement factor of approximately 61 when compared to the typical gated injection. The detection and quantification limits of the developed approach for quinine were 3.0 µg/mL and 10 µg/mL, respectively, with intraday and interday repeatability (%RSDs, n=5) of 3.6 % and 4.4 %. Recoveries in spiked human plasma were 98.1 % – 99.8 %.
format	Thesis
qualification_level	Master's degree
author	Tai, Chee Tung
author_facet	Tai, Chee Tung
author_sort	Tai, Chee Tung
title	Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
title_short	Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
title_full	Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
title_fullStr	Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
title_full_unstemmed	Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
title_sort	rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system
granting_institution	Universiti Teknologi Malaysia
granting_department	Chemistry
publishDate	2018
url	http://eprints.utm.my/id/eprint/81387/1/TaiCheeTungMFS2019.pdf
_version_	1747818319458598912

Rapid quantification of quinine by multi-stacking in a portable microchip electrophoresis system

Similar Items