Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots

Globally, 998 million tonnes of agricultural waste is disposed into landfills per year and Malaysia contributes 1.2 million tonnes of the amount. The waste consists of lignocellulosic biomass, a chainlike sugars called cellulose and hemicellulose embedded in a woody material called lignin, which cou...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohd. Yasim, Nur Hanis
Format:	Thesis
Language:	English
Published:	2018
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/81488/1/NurHanisMohdMFBME2018.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.81488
record_format	uketd_dc
spelling	my-utm-ep.814882019-08-23T05:19:08Z Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots 2018 Mohd. Yasim, Nur Hanis Q Science (General) Globally, 998 million tonnes of agricultural waste is disposed into landfills per year and Malaysia contributes 1.2 million tonnes of the amount. The waste consists of lignocellulosic biomass, a chainlike sugars called cellulose and hemicellulose embedded in a woody material called lignin, which could be converted into biofuel. Enzymes secreted by microorganisms are needed for the conversion of these materials into biofuels. One of good sources to find the microorganisms that are able to degrade the lignocellulosic materials is mangrove. It is a rich environment containing plenty of decayed plant materials, thus it could be a potential resource of highly productive and diverse microbial community. In this study, five bacterial strains have been isolated from mangrove roots collected in Tanjung Piai, Johor. From the lignocellulolytic screening, CN4, CN7 and CN10 showed positive results for cellulose degradation, CN10 and CN12 for xylan degradation, and CN20 for lignin degradation. Strain CN10 was selected for further investigation due to its abilities to degrade both cellulose and xylan. Gram-staining performed showed that CN10 is a Gram-positive rod-shaped bacterium. Based on 16S rRNAgene sequence analysis, the selected strain was identified as Exiguobacterium sp. CN10. Growth profile was carried out at 35°C, pH 7 and 5% (w/v) salt using tryptic soy broth as medium. The effect of temperature, pH and salinity on cellulose and xylan degradation using crude enzyme of CN10 were investigated. For cellulose degradation, the optimal temperature, pH and salinity for strain CN10 were 50°C, pH 8.0 and 12% (w/v) respectively. For xylanase activity, the optimal temperature and pH for the strain were also at 50°C and pH 8.0, while the optimal salinity was at 10% (w/v) salt. Collectively, the findings suggested that the strain CN10 may have a great potential in the lignocellulosic biomass degradation which could be of great commercial value. 2018 Thesis http://eprints.utm.my/id/eprint/81488/ http://eprints.utm.my/id/eprint/81488/1/NurHanisMohdMFBME2018.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:120473 masters Universiti Teknologi Malaysia Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mohd. Yasim, Nur Hanis Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
description	Globally, 998 million tonnes of agricultural waste is disposed into landfills per year and Malaysia contributes 1.2 million tonnes of the amount. The waste consists of lignocellulosic biomass, a chainlike sugars called cellulose and hemicellulose embedded in a woody material called lignin, which could be converted into biofuel. Enzymes secreted by microorganisms are needed for the conversion of these materials into biofuels. One of good sources to find the microorganisms that are able to degrade the lignocellulosic materials is mangrove. It is a rich environment containing plenty of decayed plant materials, thus it could be a potential resource of highly productive and diverse microbial community. In this study, five bacterial strains have been isolated from mangrove roots collected in Tanjung Piai, Johor. From the lignocellulolytic screening, CN4, CN7 and CN10 showed positive results for cellulose degradation, CN10 and CN12 for xylan degradation, and CN20 for lignin degradation. Strain CN10 was selected for further investigation due to its abilities to degrade both cellulose and xylan. Gram-staining performed showed that CN10 is a Gram-positive rod-shaped bacterium. Based on 16S rRNAgene sequence analysis, the selected strain was identified as Exiguobacterium sp. CN10. Growth profile was carried out at 35°C, pH 7 and 5% (w/v) salt using tryptic soy broth as medium. The effect of temperature, pH and salinity on cellulose and xylan degradation using crude enzyme of CN10 were investigated. For cellulose degradation, the optimal temperature, pH and salinity for strain CN10 were 50°C, pH 8.0 and 12% (w/v) respectively. For xylanase activity, the optimal temperature and pH for the strain were also at 50°C and pH 8.0, while the optimal salinity was at 10% (w/v) salt. Collectively, the findings suggested that the strain CN10 may have a great potential in the lignocellulosic biomass degradation which could be of great commercial value.
format	Thesis
qualification_level	Master's degree
author	Mohd. Yasim, Nur Hanis
author_facet	Mohd. Yasim, Nur Hanis
author_sort	Mohd. Yasim, Nur Hanis
title	Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
title_short	Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
title_full	Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
title_fullStr	Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
title_full_unstemmed	Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
title_sort	isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots
granting_institution	Universiti Teknologi Malaysia
granting_department	Biosciences and Medical Engineering
publishDate	2018
url	http://eprints.utm.my/id/eprint/81488/1/NurHanisMohdMFBME2018.pdf
_version_	1747818342406684672

Isolation, identification and characterisation of lignocellulolytic bacteria from mangrove roots

Similar Items