Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties

Swietenia macrophylla (S.macrophylla) or commonly known as Tunjuk Langit in Malaysia has been used as traditional remedy for diseases treatment as the seeds of S. macrophylla have been examined for its anti-inflammatory, antimutagenecity, and anti-tumour activities. The objectives of this research a...

Full description

Saved in:

Bibliographic Details
Main Author:	Abu Bakar, Noor Fadzilah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/86095/1/NoorFadzilahAbuMSChE2019.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.86095
record_format	uketd_dc
spelling	my-utm-ep.860952020-08-30T08:56:06Z Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties 2019 Abu Bakar, Noor Fadzilah TP Chemical technology Swietenia macrophylla (S.macrophylla) or commonly known as Tunjuk Langit in Malaysia has been used as traditional remedy for diseases treatment as the seeds of S. macrophylla have been examined for its anti-inflammatory, antimutagenecity, and anti-tumour activities. The objectives of this research are to optimise the extraction of the S. macrophylla seed oil by using microwave assisted extraction (MAE) and to evaluate physicochemical and biological properties of the extracted S. macrophylla seed oil. MAE provides process acceleration and higher extraction yield compared to conventional extraction. The operating parameters were optimized by response surface methodology using design expert version 6 to obtain optimum yield of S. macrophylla seed oil. Properties of S. macrophylla seed oil were characterised by using total phenolic content (TPC), antioxidant properties via DPPH (2,2-diphenyl-1-picrilhydrazyl) and ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6- sulphonic acid)) free radical scavenging method, the anti-diabetic activity via aglucosidase and a-amylase inhibition assays and the anti-microbial activity via agar disk-diffusion method. Gas chromatography-mass spectrometry (GC-MS) was used to analyse the fatty acid composition in the seed oil. The result showed that 43.69 ± 0.092 w/w % oil yield was obtained at optimal condition of 6 min irradiation time, solvent-to-solid ratio of 26 mL solvent/g solid and 460 W microwave power. The seed oil extracted by using MAE exhibited the highest TPC concentration, the best DPPH and ABTS inhibition compared to supercritical carbon dioxide extraction (SCCO2) and soxhlet extraction (SE) method. S. macrophylla seed oil demonstrated potent inhibition on a-amylase but weak inhibition towards a-glucosidase enzyme. S. macrophylla seed oil was found to have excellent inhibition zone towards Escherichia coli and Bacillus subtilis. Result from GC-MS analysis showed that the S. macrophylla seed oil was composed of fatty acids including linoleic (32.1%), oleic (57.3%) and palmitic (10.6%). These findings proved that S. macrophylla seed oil extracted using MAE could provide better quantity and quality of oil in shorter time compared to SC-CO2 and SE methods. 2019 Thesis http://eprints.utm.my/id/eprint/86095/ http://eprints.utm.my/id/eprint/86095/1/NoorFadzilahAbuMSChE2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:131584 masters Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering Faculty of Engineering - School of Chemical & Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abu Bakar, Noor Fadzilah Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
description	Swietenia macrophylla (S.macrophylla) or commonly known as Tunjuk Langit in Malaysia has been used as traditional remedy for diseases treatment as the seeds of S. macrophylla have been examined for its anti-inflammatory, antimutagenecity, and anti-tumour activities. The objectives of this research are to optimise the extraction of the S. macrophylla seed oil by using microwave assisted extraction (MAE) and to evaluate physicochemical and biological properties of the extracted S. macrophylla seed oil. MAE provides process acceleration and higher extraction yield compared to conventional extraction. The operating parameters were optimized by response surface methodology using design expert version 6 to obtain optimum yield of S. macrophylla seed oil. Properties of S. macrophylla seed oil were characterised by using total phenolic content (TPC), antioxidant properties via DPPH (2,2-diphenyl-1-picrilhydrazyl) and ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6- sulphonic acid)) free radical scavenging method, the anti-diabetic activity via aglucosidase and a-amylase inhibition assays and the anti-microbial activity via agar disk-diffusion method. Gas chromatography-mass spectrometry (GC-MS) was used to analyse the fatty acid composition in the seed oil. The result showed that 43.69 ± 0.092 w/w % oil yield was obtained at optimal condition of 6 min irradiation time, solvent-to-solid ratio of 26 mL solvent/g solid and 460 W microwave power. The seed oil extracted by using MAE exhibited the highest TPC concentration, the best DPPH and ABTS inhibition compared to supercritical carbon dioxide extraction (SCCO2) and soxhlet extraction (SE) method. S. macrophylla seed oil demonstrated potent inhibition on a-amylase but weak inhibition towards a-glucosidase enzyme. S. macrophylla seed oil was found to have excellent inhibition zone towards Escherichia coli and Bacillus subtilis. Result from GC-MS analysis showed that the S. macrophylla seed oil was composed of fatty acids including linoleic (32.1%), oleic (57.3%) and palmitic (10.6%). These findings proved that S. macrophylla seed oil extracted using MAE could provide better quantity and quality of oil in shorter time compared to SC-CO2 and SE methods.
format	Thesis
qualification_level	Master's degree
author	Abu Bakar, Noor Fadzilah
author_facet	Abu Bakar, Noor Fadzilah
author_sort	Abu Bakar, Noor Fadzilah
title	Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
title_short	Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
title_full	Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
title_fullStr	Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
title_full_unstemmed	Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
title_sort	extraction of swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties
granting_institution	Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering
granting_department	Faculty of Engineering - School of Chemical & Energy Engineering
publishDate	2019
url	http://eprints.utm.my/id/eprint/86095/1/NoorFadzilahAbuMSChE2019.pdf
_version_	1747818494752194560

Extraction of Swietenia macrophylla seed by using microwave assisted extraction technique and the characterisation of its phytochemical properties

Similar Items