Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods

Microbiologically Influenced Corrosion (MIC) or bacterial corrosion can be a major problem in process equipment exposed to different types of waters in the production, processing and handling of crude oil and gas. Sulphate-reducing bacteria (SRB) are among the most destructive environmental organism...

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Main Author:	Roslan, Norhafiza
Format:	Thesis
Language:	English
Published:	2013
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/86162/1/NorhafizaRoslanMFPREE2013.pdf
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spelling	my-utm-ep.861622020-08-30T09:04:57Z Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods 2013 Roslan, Norhafiza TP Chemical technology Microbiologically Influenced Corrosion (MIC) or bacterial corrosion can be a major problem in process equipment exposed to different types of waters in the production, processing and handling of crude oil and gas. Sulphate-reducing bacteria (SRB) are among the most destructive environmental organism and their industrial impact is widespread. They cause corrosion and stress corrosion cracking of metals and alloys used in petroleum production, cooling water system, waste water treatment and all aqueous process. Hence, there is a constant need to monitor bacterial proliferation in such systems in order to take remedial action. The most commonly used methodology to determine bacterial contamination is the Serial Dilution Technique which is culturing the sample in a laboratory until the numbers of SRB were sufficiently high to be detected by observation of general blackening of the sample. However, this method though very reliable, is quite involved and time consuming as it takes 28 days to analyze the result. So, another method is proposed to speed up this culturing process or bypass culturing altogether and monitor the organisms directly. This method is known as SRB Rapidcheck II. Results from the evaluation indicate that the SRB Rapidcheck II method provided higher SRB-population estimates in this case and results were available in 20 minutes instead of 28 days required by the NACE serial dilution method. Due to alarm activity of sulphate-reducing bacteria in the pipeline, it is recommended to conduct biocide treatment to kill SRB which is anaerobic. 2013 Thesis http://eprints.utm.my/id/eprint/86162/ http://eprints.utm.my/id/eprint/86162/1/NorhafizaRoslanMFPREE2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:131280 masters Universiti Teknologi Malaysia, Faculty of Petroleum & Renewable Energy Engineering Faculty of Petroleum & Renewable Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Roslan, Norhafiza Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods
description	Microbiologically Influenced Corrosion (MIC) or bacterial corrosion can be a major problem in process equipment exposed to different types of waters in the production, processing and handling of crude oil and gas. Sulphate-reducing bacteria (SRB) are among the most destructive environmental organism and their industrial impact is widespread. They cause corrosion and stress corrosion cracking of metals and alloys used in petroleum production, cooling water system, waste water treatment and all aqueous process. Hence, there is a constant need to monitor bacterial proliferation in such systems in order to take remedial action. The most commonly used methodology to determine bacterial contamination is the Serial Dilution Technique which is culturing the sample in a laboratory until the numbers of SRB were sufficiently high to be detected by observation of general blackening of the sample. However, this method though very reliable, is quite involved and time consuming as it takes 28 days to analyze the result. So, another method is proposed to speed up this culturing process or bypass culturing altogether and monitor the organisms directly. This method is known as SRB Rapidcheck II. Results from the evaluation indicate that the SRB Rapidcheck II method provided higher SRB-population estimates in this case and results were available in 20 minutes instead of 28 days required by the NACE serial dilution method. Due to alarm activity of sulphate-reducing bacteria in the pipeline, it is recommended to conduct biocide treatment to kill SRB which is anaerobic.
format	Thesis
qualification_level	Master's degree
author	Roslan, Norhafiza
author_facet	Roslan, Norhafiza
author_sort	Roslan, Norhafiza
title	Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods
title_short	Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods
title_full	Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods
title_fullStr	Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods
title_full_unstemmed	Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods
title_sort	detection of sulphate-reducing bacteria using nace serial dilution and srb rapidcheck ii methods
granting_institution	Universiti Teknologi Malaysia, Faculty of Petroleum & Renewable Energy Engineering
granting_department	Faculty of Petroleum & Renewable Energy Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/86162/1/NorhafizaRoslanMFPREE2013.pdf
_version_	1747818499186622464

Detection of sulphate-reducing bacteria using NACE serial dilution and SRB rapidcheck II methods

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