Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro

Ficus deltoidea (F. deltoidea) belongs to moraeeae. Vitexin and isovitexin that have high antioxidant property are considered as the chemical markers of F. deltoidea leaves. The objective of this research was to investigate the fractionation of F. deltoidea leaves using solid phase extraction on ant...

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Main Author:	Zulikram, Fatin Husna
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/91974/1/FatinHusnaZulikramMSChE2019.pdf
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spelling	my-utm-ep.919742021-08-09T08:46:44Z Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro 2019 Zulikram, Fatin Husna TP Chemical technology Ficus deltoidea (F. deltoidea) belongs to moraeeae. Vitexin and isovitexin that have high antioxidant property are considered as the chemical markers of F. deltoidea leaves. The objective of this research was to investigate the fractionation of F. deltoidea leaves using solid phase extraction on anti-ageing activity in vitro. The leaves of F. deltoidea were extracted by methanol by using ultrasonic extraction method and further fractionated using different concentration of methanol. Antioxidant activity of F. deltoidea leaves fractions and extract was analyzed through 2,2-diphenyl-1-picrylhrazyl (DPPH) scavenging ability test and total flavanoid content (TFC) activity. The result shows that 80% methanol 20% water fraction (F4) have higher percentage of scavenging activity and flavonoid content with 87.16% and 237.57 mg, respectively. The quantification of biomakers (vitexin and isovitexin) was performed using high performance liquid chromatography (HPLC) and the result showed that F4 fraction have higher percentage of vitexin (11.02%) and isovitexin (0.49%) compared to other fractions and extract. Moreover, cytotoxicity study on human skin fibroblasts cell (HSF 1184) also demonstrated that F4 has higher percentage of cell viability with value 175.29% at 100 \|ig/mL. The anti-ageing activity of F. deltoidea leaves fractions and extract were further evaluated using sircol collagen assay, inhibition of elastase assay, hyaluronidase assay and lipoxygenase assay, where F4 also showed the strongest activities compared to other fractions and extract for all assays with the value of 8.9 jig collagen concentration, 83%, 97.82%, and 88.56%, respectively. Taken together, as F4 contains high amount of vitexin and isovitexin, therefore these compounds have the potential to be further developed as anti-ageing agent. 2019 Thesis http://eprints.utm.my/id/eprint/91974/ http://eprints.utm.my/id/eprint/91974/1/FatinHusnaZulikramMSChE2019.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:132080 masters Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering Faculty of Engineering - School of Chemical & Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Zulikram, Fatin Husna Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
description	Ficus deltoidea (F. deltoidea) belongs to moraeeae. Vitexin and isovitexin that have high antioxidant property are considered as the chemical markers of F. deltoidea leaves. The objective of this research was to investigate the fractionation of F. deltoidea leaves using solid phase extraction on anti-ageing activity in vitro. The leaves of F. deltoidea were extracted by methanol by using ultrasonic extraction method and further fractionated using different concentration of methanol. Antioxidant activity of F. deltoidea leaves fractions and extract was analyzed through 2,2-diphenyl-1-picrylhrazyl (DPPH) scavenging ability test and total flavanoid content (TFC) activity. The result shows that 80% methanol 20% water fraction (F4) have higher percentage of scavenging activity and flavonoid content with 87.16% and 237.57 mg, respectively. The quantification of biomakers (vitexin and isovitexin) was performed using high performance liquid chromatography (HPLC) and the result showed that F4 fraction have higher percentage of vitexin (11.02%) and isovitexin (0.49%) compared to other fractions and extract. Moreover, cytotoxicity study on human skin fibroblasts cell (HSF 1184) also demonstrated that F4 has higher percentage of cell viability with value 175.29% at 100 \|ig/mL. The anti-ageing activity of F. deltoidea leaves fractions and extract were further evaluated using sircol collagen assay, inhibition of elastase assay, hyaluronidase assay and lipoxygenase assay, where F4 also showed the strongest activities compared to other fractions and extract for all assays with the value of 8.9 jig collagen concentration, 83%, 97.82%, and 88.56%, respectively. Taken together, as F4 contains high amount of vitexin and isovitexin, therefore these compounds have the potential to be further developed as anti-ageing agent.
format	Thesis
qualification_level	Master's degree
author	Zulikram, Fatin Husna
author_facet	Zulikram, Fatin Husna
author_sort	Zulikram, Fatin Husna
title	Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
title_short	Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
title_full	Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
title_fullStr	Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
title_full_unstemmed	Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
title_sort	fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro
granting_institution	Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering
granting_department	Faculty of Engineering - School of Chemical & Energy Engineering
publishDate	2019
url	http://eprints.utm.my/id/eprint/91974/1/FatinHusnaZulikramMSChE2019.pdf
_version_	1747818546559188992

Fractionation of ficus deltoidea leaves using solid phase extraction on anti-ageing activity in vitro

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