Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor

The performance of a biosensor sensing layer is mostly determined by the immobilization method and the materials used to immobilize the enzyme. In this work, the performances of four types of glucose oxidase immobilization materials based on poly(vinyl alcohol) (PVA) for peroxide-based glucose biose...

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Main Author:	Wong, Fui Ling
Format:	Thesis
Language:	English
Published:	2008
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/9648/1/WongFuiLingMFKT2008.pdf
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spelling	my-utm-ep.96482018-10-14T07:19:59Z Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor 2008-07 Wong, Fui Ling TP Chemical technology The performance of a biosensor sensing layer is mostly determined by the immobilization method and the materials used to immobilize the enzyme. In this work, the performances of four types of glucose oxidase immobilization materials based on poly(vinyl alcohol) (PVA) for peroxide-based glucose biosensor were compared. The matrices of interest were glutaraldehyde cross-linked PVA (GAPVA), freeze-thawed PVA cryogel (FTPVA), tetramethoxysilane sol-gel-PVA hybrid material (TMOS-PVA), and alumina sol-gel-PVA hybrid material (Al-PVA). Only GAPVA showed short period of enzyme leaking and high value of Km app. However, its sensitivity was poor. With the same enzyme loading, the other three types of membranes showed good sensitivity and stability. FTPVA and TMOSPVA, which showed satisfactory sensitivity and adequate value of Km app were quite promising as the support materials for immobilizing glucose oxidase (GOD). The enzyme leakage of FTPVA which had shown highest leaking was improved by reducing the enzyme loading. Even though peroxide-based sensor is very simple and easy to construct, it suffers from electrochemical interferences from common electroactive species present in blood such as acetaminophen. Hence, a photocured poly(hydroxyethyl methacrylate) (pHEMA) layer was investigated as a potential permselective inner membrane to eliminate the interferences based on size exclusion. PHEMA membrane with the cross-linking ratio of 0.043 was found to be able to achieve a selectivity of 10, while maintaining an acceptable degree of hydrogen peroxide response. In a two-layer glucose biosensor, where the FTPVA-GOD and cross-linked pHEMA were combined, acetaminophen and ascorbic acid at 0.2 mM were eliminated completely. 0.2 mM uric acid still gave a bias of approximately 6.6% relative to 5 mM glucose. Overall, the partial glucose biosensor showed promising performance. 2008-07 Thesis http://eprints.utm.my/id/eprint/9648/ http://eprints.utm.my/id/eprint/9648/1/WongFuiLingMFKT2008.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:1084 masters Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering Faculty of Chemical and Natural Resources Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Wong, Fui Ling Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
description	The performance of a biosensor sensing layer is mostly determined by the immobilization method and the materials used to immobilize the enzyme. In this work, the performances of four types of glucose oxidase immobilization materials based on poly(vinyl alcohol) (PVA) for peroxide-based glucose biosensor were compared. The matrices of interest were glutaraldehyde cross-linked PVA (GAPVA), freeze-thawed PVA cryogel (FTPVA), tetramethoxysilane sol-gel-PVA hybrid material (TMOS-PVA), and alumina sol-gel-PVA hybrid material (Al-PVA). Only GAPVA showed short period of enzyme leaking and high value of Km app. However, its sensitivity was poor. With the same enzyme loading, the other three types of membranes showed good sensitivity and stability. FTPVA and TMOSPVA, which showed satisfactory sensitivity and adequate value of Km app were quite promising as the support materials for immobilizing glucose oxidase (GOD). The enzyme leakage of FTPVA which had shown highest leaking was improved by reducing the enzyme loading. Even though peroxide-based sensor is very simple and easy to construct, it suffers from electrochemical interferences from common electroactive species present in blood such as acetaminophen. Hence, a photocured poly(hydroxyethyl methacrylate) (pHEMA) layer was investigated as a potential permselective inner membrane to eliminate the interferences based on size exclusion. PHEMA membrane with the cross-linking ratio of 0.043 was found to be able to achieve a selectivity of 10, while maintaining an acceptable degree of hydrogen peroxide response. In a two-layer glucose biosensor, where the FTPVA-GOD and cross-linked pHEMA were combined, acetaminophen and ascorbic acid at 0.2 mM were eliminated completely. 0.2 mM uric acid still gave a bias of approximately 6.6% relative to 5 mM glucose. Overall, the partial glucose biosensor showed promising performance.
format	Thesis
qualification_level	Master's degree
author	Wong, Fui Ling
author_facet	Wong, Fui Ling
author_sort	Wong, Fui Ling
title	Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
title_short	Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
title_full	Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
title_fullStr	Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
title_full_unstemmed	Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
title_sort	enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering
granting_department	Faculty of Chemical and Natural Resources Engineering
publishDate	2008
url	http://eprints.utm.my/id/eprint/9648/1/WongFuiLingMFKT2008.pdf
_version_	1747814769046323200

Enzyme immobilization and permselectivity analysis of an interference free peroxide based glucose biosensor

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