The yield and biological activity (LC50) of rotenone extracted from Derris elliptica

The objective of this research was to determine the effect of the processing parameters on the extraction yield of rotenoids resin, rotenone and their biological activities (LC50). The research was divided into three stages: preliminary, optimization and verification phases. Preliminary study was ca...

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Main Author:	Zubairi, Saiful Irwan
Format:	Thesis
Language:	English
Published:	2006
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/9826/1/SaifulIrwanZubairiMFKT2006.pdf
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spelling	my-utm-ep.98262018-09-17T03:46:54Z The yield and biological activity (LC50) of rotenone extracted from Derris elliptica 2006-08 Zubairi, Saiful Irwan TP Chemical technology The objective of this research was to determine the effect of the processing parameters on the extraction yield of rotenoids resin, rotenone and their biological activities (LC50). The research was divided into three stages: preliminary, optimization and verification phases. Preliminary study was carried out to determine the most appropriate processing parameters for the optimization study. The optimization study was carried out using a Central Composite Design (CCD) employing the Design-ExpertÂ® software version 6.0 to determine the effects of processing parameters on the three selected response variables which were the yield of rotenoids resin, yield of rotenone and biological activity (LC50) of rotenoids resin. The processing parameters studied were the types of solvent (acetone, chloroform and ethanol), solvent-to-solid ratio (2.0 ml/g to 10.0 ml/g) and raw material particles size (0.5 mm to 5.0 mm in diameter). The theoretical maximum yield of rotenoids resin in dried roots obtained from the optimization phase was 12.26 % (w/w) and 5.99 % (w/w) for the rotenone. The multiple response variables analysis have consistently verified the theoretical results in the range of 3.62 ml/g to 4.72 ml/g (solvent-to-solid ratio) and 0.83 mm to 1.41 mm in diameter (raw material particles size) using the acetone extract. The biological activity (LC50) value of rotenoids resin was indirectly correlated to the optimum processing parameters due to inconsistency of rotenone content (mg) and the low value of LC50 which was less than 100 ppm for all treatments. This is due to the presence of other constituents in the rotenoids resin (tephrosin, 12aÃŸ-rotenolone and deguelin) which contributed to the low LC50 values. The optimization of the processing parameters resulted in an increase of yield of rotenoids resin but reduced yield of rotenone 2006-08 Thesis http://eprints.utm.my/id/eprint/9826/ http://eprints.utm.my/id/eprint/9826/1/SaifulIrwanZubairiMFKT2006.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:1224 masters Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering Faculty of Chemical and Natural Resources Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Zubairi, Saiful Irwan The yield and biological activity (LC50) of rotenone extracted from Derris elliptica
description	The objective of this research was to determine the effect of the processing parameters on the extraction yield of rotenoids resin, rotenone and their biological activities (LC50). The research was divided into three stages: preliminary, optimization and verification phases. Preliminary study was carried out to determine the most appropriate processing parameters for the optimization study. The optimization study was carried out using a Central Composite Design (CCD) employing the Design-ExpertÂ® software version 6.0 to determine the effects of processing parameters on the three selected response variables which were the yield of rotenoids resin, yield of rotenone and biological activity (LC50) of rotenoids resin. The processing parameters studied were the types of solvent (acetone, chloroform and ethanol), solvent-to-solid ratio (2.0 ml/g to 10.0 ml/g) and raw material particles size (0.5 mm to 5.0 mm in diameter). The theoretical maximum yield of rotenoids resin in dried roots obtained from the optimization phase was 12.26 % (w/w) and 5.99 % (w/w) for the rotenone. The multiple response variables analysis have consistently verified the theoretical results in the range of 3.62 ml/g to 4.72 ml/g (solvent-to-solid ratio) and 0.83 mm to 1.41 mm in diameter (raw material particles size) using the acetone extract. The biological activity (LC50) value of rotenoids resin was indirectly correlated to the optimum processing parameters due to inconsistency of rotenone content (mg) and the low value of LC50 which was less than 100 ppm for all treatments. This is due to the presence of other constituents in the rotenoids resin (tephrosin, 12aÃŸ-rotenolone and deguelin) which contributed to the low LC50 values. The optimization of the processing parameters resulted in an increase of yield of rotenoids resin but reduced yield of rotenone
format	Thesis
qualification_level	Master's degree
author	Zubairi, Saiful Irwan
author_facet	Zubairi, Saiful Irwan
author_sort	Zubairi, Saiful Irwan
title	The yield and biological activity (LC50) of rotenone extracted from Derris elliptica
title_short	The yield and biological activity (LC50) of rotenone extracted from Derris elliptica
title_full	The yield and biological activity (LC50) of rotenone extracted from Derris elliptica
title_fullStr	The yield and biological activity (LC50) of rotenone extracted from Derris elliptica
title_full_unstemmed	The yield and biological activity (LC50) of rotenone extracted from Derris elliptica
title_sort	yield and biological activity (lc50) of rotenone extracted from derris elliptica
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering
granting_department	Faculty of Chemical and Natural Resources Engineering
publishDate	2006
url	http://eprints.utm.my/id/eprint/9826/1/SaifulIrwanZubairiMFKT2006.pdf
_version_	1747814780840706048

The yield and biological activity (LC50) of rotenone extracted from Derris elliptica

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