Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.

Curcuma xanthorrhiza belongs to the Zingiberaceae family is a rich source of phenolics and terpenoids with various bioactivities. The essential oil from the rhizomes was obtained by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Xanthorrh...

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Main Author: Azemi, Najiba
Format: Thesis
Language:English
Published: 2020
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Online Access:http://eprints.utm.my/id/eprint/99529/1/NajebaAzemiMFS2020.pdf.pdf
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spelling my-utm-ep.995292023-03-01T07:32:51Z Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb. 2020 Azemi, Najiba QD Chemistry Curcuma xanthorrhiza belongs to the Zingiberaceae family is a rich source of phenolics and terpenoids with various bioactivities. The essential oil from the rhizomes was obtained by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Xanthorrhizol (1), ar-curcumene (4), and a-cedrene (18) were found as the most abundant constituents in the essential oil. Optimization of the ultrasound-assisted extraction (UAE) of the extract from the rhizomes of C. xanthorrhiza was carried out via response surface methodology (RSM). Seventeen experiments were developed with Box-Behnken design (BBD) using three variables which are extraction time (5, 12.50, 20 min), temperature (30, 40, 50°C), and liquid-solid ratio (6, 8, 10 mL/g), respectively. The effect of parameters on the responses of extraction yield, quantification of xanthorrhizol (1), and curcumin (2) was determined. In the extracts, xanthorrhizol (1) and curcumin (2) were quantified using reversed-phase high-performance liquid chromatography (RP HPLC) combined with a photodiode array detector. The highest percentage of yield (72.20%) and the concentration of xanthorrhizol (1) (85.68% w/w) were found at the extraction temperature of 50°C, time of 20 minutes and 8 mL/g liquid-solid ratio. The highest concentration of curcumin (2) (39.73% w/w) was found at the extraction temperature of 30°C, time of 12.50 minutes, and 10 mL/g liquid-solid ratio. Experimental values are closely agreed with the predicted values. The determination coefficient (R2) of extraction yield, xanthorrhizol (1), and curcumin (2) are 0.9960, 0.9983, and 0.9990, respectively. The variance analysis (ANOVA) showed good quadratic model. The numerical data suggested the success of the RSM method in optimizing the phytochemicals extraction parameters from the rhizomes of C. xanthorrhiza. 2020 Thesis http://eprints.utm.my/id/eprint/99529/ http://eprints.utm.my/id/eprint/99529/1/NajebaAzemiMFS2020.pdf.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146099 masters Universiti Teknologi Malaysia Faculty of Engineering - School of Chemical & Energy Engineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic QD Chemistry
spellingShingle QD Chemistry
Azemi, Najiba
Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.
description Curcuma xanthorrhiza belongs to the Zingiberaceae family is a rich source of phenolics and terpenoids with various bioactivities. The essential oil from the rhizomes was obtained by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Xanthorrhizol (1), ar-curcumene (4), and a-cedrene (18) were found as the most abundant constituents in the essential oil. Optimization of the ultrasound-assisted extraction (UAE) of the extract from the rhizomes of C. xanthorrhiza was carried out via response surface methodology (RSM). Seventeen experiments were developed with Box-Behnken design (BBD) using three variables which are extraction time (5, 12.50, 20 min), temperature (30, 40, 50°C), and liquid-solid ratio (6, 8, 10 mL/g), respectively. The effect of parameters on the responses of extraction yield, quantification of xanthorrhizol (1), and curcumin (2) was determined. In the extracts, xanthorrhizol (1) and curcumin (2) were quantified using reversed-phase high-performance liquid chromatography (RP HPLC) combined with a photodiode array detector. The highest percentage of yield (72.20%) and the concentration of xanthorrhizol (1) (85.68% w/w) were found at the extraction temperature of 50°C, time of 20 minutes and 8 mL/g liquid-solid ratio. The highest concentration of curcumin (2) (39.73% w/w) was found at the extraction temperature of 30°C, time of 12.50 minutes, and 10 mL/g liquid-solid ratio. Experimental values are closely agreed with the predicted values. The determination coefficient (R2) of extraction yield, xanthorrhizol (1), and curcumin (2) are 0.9960, 0.9983, and 0.9990, respectively. The variance analysis (ANOVA) showed good quadratic model. The numerical data suggested the success of the RSM method in optimizing the phytochemicals extraction parameters from the rhizomes of C. xanthorrhiza.
format Thesis
qualification_level Master's degree
author Azemi, Najiba
author_facet Azemi, Najiba
author_sort Azemi, Najiba
title Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.
title_short Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.
title_full Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.
title_fullStr Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.
title_full_unstemmed Extraction and optimization of phytochemicals from Curcuma xanthorrhiza Roxb.
title_sort extraction and optimization of phytochemicals from curcuma xanthorrhiza roxb.
granting_institution Universiti Teknologi Malaysia
granting_department Faculty of Engineering - School of Chemical & Energy Engineering
publishDate 2020
url http://eprints.utm.my/id/eprint/99529/1/NajebaAzemiMFS2020.pdf.pdf
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