Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold

Bacterial infection can restrict and delay the wound healing process. In this study, three different kinds of natural antibacterial agents (NAA), i.e., chitosan (Cs), epsilon poly-L-lysine (EPL) and curcumin (Cur), were used with a membrane containing polyvinyl alcohol (PVA) and polycaprolactone (PC...

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Main Author:	Tan, Yong Chee
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/99534/1/TanYongCheePhDFS2021.pdf.pdf
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spelling	my-utm-ep.995342023-03-05T06:49:11Z Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold 2021 Tan, Yong Chee QD Chemistry Bacterial infection can restrict and delay the wound healing process. In this study, three different kinds of natural antibacterial agents (NAA), i.e., chitosan (Cs), epsilon poly-L-lysine (EPL) and curcumin (Cur), were used with a membrane containing polyvinyl alcohol (PVA) and polycaprolactone (PCL), for inhibiting the bacterial growth meanwhile enhancing cell growth. Electrospinning was chosen to fabricate the membrane as this technique produces a nanofibrous membrane that mimics the extracellular matrix (ECM) of human tissue. Moreover, in order to increase the biocompatibility of the membrane, solution formulations containing glacial and 80% (v/v) of acetic acid were introduced to prepare PCL and PVA solutions, respectively, before mixing and adding the NAA. In this study, all electrospun nanofibrous membranes exhibited the chemical functional group of hydrophilic PVA and hydrophobic PCL. The presence of those chemical functional groups in the membranes is essential for achieving ideal scaffold properties. The presence of NAA in the membranes was further confirmed by investigating the presence of nitrogen through carbon, hydrogen, nitrogen and sulphur elemental analysis (CHNS). Overall, all electrospun nanofibrous membranes have tiny fibre diameter (92-176 nm), high porosity (77-89%), surface with hydrophilic property (0-27°), moderate body fluid uptake (209-329%) and controllable degradation rate. Although the water vapour transmission rate in the range of 1435 to 1620 g/m2/day was relatively lower than the recommended value, this did not restrict them to act as a barrier to prevent excessive water loss during wound healing. The antibacterial studies indicated that the electropun membrane containing NAA inhibited the growth of S. aureus and E. coli. Among them, electropun PVA*-PCL-EPL showed almost 99.9% of bacterial reduction after 1 h of treatment. Finally, the in vitro and in vivo studies were also conducted to confirm the effectiveness of the electrospun nanofibrous membrane in wound healing. In conclusion, all electrospun nanofibrous membranes containing PVA, PCL and NAA could be applied as a scaffold for skin tissue regeneration. 2021 Thesis http://eprints.utm.my/id/eprint/99534/ http://eprints.utm.my/id/eprint/99534/1/TanYongCheePhDFS2021.pdf.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146065 phd doctoral Universiti Teknologi Malaysia Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Tan, Yong Chee Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
description	Bacterial infection can restrict and delay the wound healing process. In this study, three different kinds of natural antibacterial agents (NAA), i.e., chitosan (Cs), epsilon poly-L-lysine (EPL) and curcumin (Cur), were used with a membrane containing polyvinyl alcohol (PVA) and polycaprolactone (PCL), for inhibiting the bacterial growth meanwhile enhancing cell growth. Electrospinning was chosen to fabricate the membrane as this technique produces a nanofibrous membrane that mimics the extracellular matrix (ECM) of human tissue. Moreover, in order to increase the biocompatibility of the membrane, solution formulations containing glacial and 80% (v/v) of acetic acid were introduced to prepare PCL and PVA solutions, respectively, before mixing and adding the NAA. In this study, all electrospun nanofibrous membranes exhibited the chemical functional group of hydrophilic PVA and hydrophobic PCL. The presence of those chemical functional groups in the membranes is essential for achieving ideal scaffold properties. The presence of NAA in the membranes was further confirmed by investigating the presence of nitrogen through carbon, hydrogen, nitrogen and sulphur elemental analysis (CHNS). Overall, all electrospun nanofibrous membranes have tiny fibre diameter (92-176 nm), high porosity (77-89%), surface with hydrophilic property (0-27°), moderate body fluid uptake (209-329%) and controllable degradation rate. Although the water vapour transmission rate in the range of 1435 to 1620 g/m2/day was relatively lower than the recommended value, this did not restrict them to act as a barrier to prevent excessive water loss during wound healing. The antibacterial studies indicated that the electropun membrane containing NAA inhibited the growth of S. aureus and E. coli. Among them, electropun PVA*-PCL-EPL showed almost 99.9% of bacterial reduction after 1 h of treatment. Finally, the in vitro and in vivo studies were also conducted to confirm the effectiveness of the electrospun nanofibrous membrane in wound healing. In conclusion, all electrospun nanofibrous membranes containing PVA, PCL and NAA could be applied as a scaffold for skin tissue regeneration.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Tan, Yong Chee
author_facet	Tan, Yong Chee
author_sort	Tan, Yong Chee
title	Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
title_short	Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
title_full	Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
title_fullStr	Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
title_full_unstemmed	Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
title_sort	fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Science
publishDate	2021
url	http://eprints.utm.my/id/eprint/99534/1/TanYongCheePhDFS2021.pdf.pdf
_version_	1776100613689442304

Fabrication of electrospun polyvinyl alcohol polycaprolactone-natural antibacterial agent blended nanofibrous membrane for skin tissue scaffold

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