Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota

Tapered single mode fiber (SMF) immobilized with glucose oxidase enzyme (GOD) and gold nanoparticles (AuNP) for recognition of glucose and sucrose, respectively have been proposed in this work. Tapered fiber is fabricated using flame heating technique and is coated to improve the sensitivity of fibe...

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Main Author:	Multar, Esmafatinsyafiqa
Format:	Thesis
Language:	English
Published:	2020
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/99579/1/EsmafatinsyafiqaMultarMFS2020.pdf.pdf
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spelling	my-utm-ep.995792023-03-05T07:27:28Z Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota 2020 Multar, Esmafatinsyafiqa Q Science (General) Tapered single mode fiber (SMF) immobilized with glucose oxidase enzyme (GOD) and gold nanoparticles (AuNP) for recognition of glucose and sucrose, respectively have been proposed in this work. Tapered fiber is fabricated using flame heating technique and is coated to improve the sensitivity of fiber based sensor. 3-(aminopropyl) triethoxysilane (APTES), GOD and AuNP are functionalized onto the tapered region of SMF through covalent interaction. The purpose of this study is to develop coated tapered fiber for determining the sensitivity and selectivity of glucose and sucrose in different concentration of glucose and sucrose solutions and in different types of Daucus carota. Solution concentration of 0.1 g/ml, 0.2 g/ml and 0.3 g/ml were used to differentiate the spectrum intensity of the fiber towards different amount of glucose and sucrose in the solutions. Baby carrots, imported carrots and organic carrots were used as an indicator to prove the existence of glucose and sucrose in these different types of Daucus carota. The experimental results demonstrate the sensitivities of GOD-immobilized fiber were 0.01045 , 0.11415 , 0.12689 and 0.16807 towards glucose solution, baby carrots, organic carrots and imported carrots, respectively. Meanwhile, the sensitivities of AuNP-immobilized fiber towards sucrose solution, baby carrots, imported carrots and organic carrots were 0.00840 , 0.000483 , 0.000698 and 0.000777 , respectively. The slopes obtained from the graph represent the sensitivities of the fibers towards different types of testing samples. 2020 Thesis http://eprints.utm.my/id/eprint/99579/ http://eprints.utm.my/id/eprint/99579/1/EsmafatinsyafiqaMultarMFS2020.pdf.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146105 masters Universiti Teknologi Malaysia Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Multar, Esmafatinsyafiqa Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
description	Tapered single mode fiber (SMF) immobilized with glucose oxidase enzyme (GOD) and gold nanoparticles (AuNP) for recognition of glucose and sucrose, respectively have been proposed in this work. Tapered fiber is fabricated using flame heating technique and is coated to improve the sensitivity of fiber based sensor. 3-(aminopropyl) triethoxysilane (APTES), GOD and AuNP are functionalized onto the tapered region of SMF through covalent interaction. The purpose of this study is to develop coated tapered fiber for determining the sensitivity and selectivity of glucose and sucrose in different concentration of glucose and sucrose solutions and in different types of Daucus carota. Solution concentration of 0.1 g/ml, 0.2 g/ml and 0.3 g/ml were used to differentiate the spectrum intensity of the fiber towards different amount of glucose and sucrose in the solutions. Baby carrots, imported carrots and organic carrots were used as an indicator to prove the existence of glucose and sucrose in these different types of Daucus carota. The experimental results demonstrate the sensitivities of GOD-immobilized fiber were 0.01045 , 0.11415 , 0.12689 and 0.16807 towards glucose solution, baby carrots, organic carrots and imported carrots, respectively. Meanwhile, the sensitivities of AuNP-immobilized fiber towards sucrose solution, baby carrots, imported carrots and organic carrots were 0.00840 , 0.000483 , 0.000698 and 0.000777 , respectively. The slopes obtained from the graph represent the sensitivities of the fibers towards different types of testing samples.
format	Thesis
qualification_level	Master's degree
author	Multar, Esmafatinsyafiqa
author_facet	Multar, Esmafatinsyafiqa
author_sort	Multar, Esmafatinsyafiqa
title	Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
title_short	Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
title_full	Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
title_fullStr	Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
title_full_unstemmed	Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
title_sort	fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Science
publishDate	2020
url	http://eprints.utm.my/id/eprint/99579/1/EsmafatinsyafiqaMultarMFS2020.pdf.pdf
_version_	1776100621292666880

Fiber optic sensor functionalized with glucose oxidase and gold nanoparticles for glucose and sucrose detections in daucus carota

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