Formulation of antimicrobial cream extracted from Chromolaena odorata (Pokok Kapal Terbang) for wound healing
<p>Chromolaena odorata has reported to have medicinal value due to the presence of</p><p>active compounds which have antimicrobial and wound healing properties. Therefore,</p><p>this study was carried out to formulate the antimicr...
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| Format: | thesis |
| Language: | eng |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=10579 |
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| Summary: | <p>Chromolaena odorata has reported to have medicinal value due to the presence of</p><p>active compounds which have antimicrobial and wound healing properties. Therefore,</p><p>this study was carried out to formulate the antimicrobial cream from C. odorata</p><p>extract used for wound healing treatment. The study aims to screen the secondary</p><p>metabolites and determine C. odorata extract concentration for antimicrobial activity</p><p>to be used in cream formulation. The leaves were extracted in 95% methanol and 95%</p><p>ethanol for secondary metabolite screening and Gas Chromatography-Mass</p><p>Spectrometry. Chromolaena odorata extract with a concentration of 5%, 10%, 15%</p><p>and 20% was performed for antimicrobial activity tested on Staphylococcus aureus,</p><p>Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Candida albicans.</p><p>The best of C. odorata extract concentration was used in six formulations of</p><p>antimicrobial cream. The stability test was performed on all cream formulations. The</p><p>methanolic extract was present with tannin, terpenoid, phenolic acids, alkaloid, and</p><p>saponin while ethanolic extract was present with tannin and alkaloid. Both extracts</p><p>are absent with flavonoid compounds. The GC-MS chromatogram identified as many</p><p>as 26 and 15 compounds for ethanolic and methanolic extract, respectively. The C.</p><p>odorata ethanolic extract consists mainly of Fatty acid (39.2%), Sesquiterpenoid</p><p>(20.69%) and Phenol (0.51%). Meanwhile, C. odorata methanolic extract consists of</p><p>Sesquiterpenoid (62.26%) and Fatty acid (17.11%). The best concentration was 20%</p><p>C. odorata methanolic extract had the highest diameter of inhibition zone against E.</p><p>coli and C. albicans at 20.67 mm and 13.3 mm, respectively. Formulation of F4, F5,</p><p>and F6 was stable in physicochemical and organoleptic studies. No significant</p><p>difference for F1 and F3 in pH measurement; F2, F3, F5 and F6 in antibacterial</p><p>activity and F1, F3, and F5 in antifungal activity. In conclusion, out of the six</p><p>formulations, the F3 formulation has optimal stability and is suitable as an</p><p>antimicrobial cream for wound healing treatment.</p> |
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