Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia
<p>The objective of this study is to develop species-specific environmental DNA (eDNA)</p><p>markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene</p><p>sequences. Validation of the developed species-speci...
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| Format: | thesis |
| Language: | eng |
| Published: |
2023
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=11144 |
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| Summary: | <p>The objective of this study is to develop species-specific environmental DNA (eDNA)</p><p>markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene</p><p>sequences. Validation of the developed species-specific eDNA markers were done exsitu</p><p>for environmental sample from Tasik Raban. New digital PCR (dPCR) method was</p><p>also employed to detect the presence of Cichla ocellaris, and Cichla kelberi in</p><p>environmental water samples. Result from this study showed that COI gene can be used</p><p>to develop species-specific markers for species Cichla. Ex-situ validation results also</p><p>showed that the developed markers CO1 amplified specifically to specimen C. ocellaris</p><p>while CK1 amplified directly to specimen of C. kelberi. In addition, dPCR supported</p><p>the specificity analysis as absolute fluorescent quantification detected the presence of</p><p>C. ocellaris and C. kelberi eDNA in water sample at 90% detection confidence. In</p><p>conclusion, CO1 and CK1 markers were successfully developed and specific to be used</p><p>as eDNA markers for C. ocellaris and C. kelberi respectively. As an implication, eDNA</p><p>analysis with species-specific markers suitable to be used as new rapid monitoring tool</p><p>in detection of invasive species for biodiversity management and conservation</p><p>purposes.</p> |
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