Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia
<p>The objective of this study is to develop species-specific environmental DNA (eDNA)</p><p>markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene</p><p>sequences. Validation of the developed species-speci...
Saved in:
Main Author: | |
---|---|
Format: | thesis |
Language: | eng |
Published: |
2023
|
Subjects: | |
Online Access: | https://ir.upsi.edu.my/detailsg.php?det=11144 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:ir.upsi.edu.my:11144 |
---|---|
record_format |
uketd_dc |
spelling |
oai:ir.upsi.edu.my:111442024-07-23 Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia 2023 Nurul Fizatul Nabilah Osman TD Environmental technology. Sanitary engineering <p>The objective of this study is to develop species-specific environmental DNA (eDNA)</p><p>markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene</p><p>sequences. Validation of the developed species-specific eDNA markers were done exsitu</p><p>for environmental sample from Tasik Raban. New digital PCR (dPCR) method was</p><p>also employed to detect the presence of Cichla ocellaris, and Cichla kelberi in</p><p>environmental water samples. Result from this study showed that COI gene can be used</p><p>to develop species-specific markers for species Cichla. Ex-situ validation results also</p><p>showed that the developed markers CO1 amplified specifically to specimen C. ocellaris</p><p>while CK1 amplified directly to specimen of C. kelberi. In addition, dPCR supported</p><p>the specificity analysis as absolute fluorescent quantification detected the presence of</p><p>C. ocellaris and C. kelberi eDNA in water sample at 90% detection confidence. In</p><p>conclusion, CO1 and CK1 markers were successfully developed and specific to be used</p><p>as eDNA markers for C. ocellaris and C. kelberi respectively. As an implication, eDNA</p><p>analysis with species-specific markers suitable to be used as new rapid monitoring tool</p><p>in detection of invasive species for biodiversity management and conservation</p><p>purposes.</p> 2023 thesis https://ir.upsi.edu.my/detailsg.php?det=11144 https://ir.upsi.edu.my/detailsg.php?det=11144 text eng openAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A |
institution |
Universiti Pendidikan Sultan Idris |
collection |
UPSI Digital Repository |
language |
eng |
topic |
TD Environmental technology Sanitary engineering |
spellingShingle |
TD Environmental technology Sanitary engineering Nurul Fizatul Nabilah Osman Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
description |
<p>The objective of this study is to develop species-specific environmental DNA (eDNA)</p><p>markers for Cichla spp. based on mitochondrial cytochrome oxidase I (COI) gene</p><p>sequences. Validation of the developed species-specific eDNA markers were done exsitu</p><p>for environmental sample from Tasik Raban. New digital PCR (dPCR) method was</p><p>also employed to detect the presence of Cichla ocellaris, and Cichla kelberi in</p><p>environmental water samples. Result from this study showed that COI gene can be used</p><p>to develop species-specific markers for species Cichla. Ex-situ validation results also</p><p>showed that the developed markers CO1 amplified specifically to specimen C. ocellaris</p><p>while CK1 amplified directly to specimen of C. kelberi. In addition, dPCR supported</p><p>the specificity analysis as absolute fluorescent quantification detected the presence of</p><p>C. ocellaris and C. kelberi eDNA in water sample at 90% detection confidence. In</p><p>conclusion, CO1 and CK1 markers were successfully developed and specific to be used</p><p>as eDNA markers for C. ocellaris and C. kelberi respectively. As an implication, eDNA</p><p>analysis with species-specific markers suitable to be used as new rapid monitoring tool</p><p>in detection of invasive species for biodiversity management and conservation</p><p>purposes.</p> |
format |
thesis |
qualification_name |
|
qualification_level |
Master's degree |
author |
Nurul Fizatul Nabilah Osman |
author_facet |
Nurul Fizatul Nabilah Osman |
author_sort |
Nurul Fizatul Nabilah Osman |
title |
Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
title_short |
Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
title_full |
Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
title_fullStr |
Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
title_full_unstemmed |
Species-specific eDNA markers for rapid detection of invasive fishes in Tasik Raban, Malaysia |
title_sort |
species-specific edna markers for rapid detection of invasive fishes in tasik raban, malaysia |
granting_institution |
Universiti Pendidikan Sultan Idris |
granting_department |
Fakulti Sains dan Matematik |
publishDate |
2023 |
url |
https://ir.upsi.edu.my/detailsg.php?det=11144 |
_version_ |
1811774756469342208 |