Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)

This study was aimed to extract and identify chemical compounds isolated from the leaves and barks of Kopsia singapurensis. The isolated compounds were evaluated for cytotoxic (MCF-7), antibacterial (Bacillus cereus) and antioxidant activity (DPPH). The isolation process was carried out using chroma...

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Main Author:	Lee, Yean Shan
Format:	thesis
Language:	eng
Published:	2015
Subjects:	QD Chemistry
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=1668
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spelling	oai:ir.upsi.edu.my:16682020-02-27 Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR) 2015 Lee, Yean Shan QD Chemistry This study was aimed to extract and identify chemical compounds isolated from the leaves and barks of Kopsia singapurensis. The isolated compounds were evaluated for cytotoxic (MCF-7), antibacterial (Bacillus cereus) and antioxidant activity (DPPH). The isolation process was carried out using chromatographic techniques such as column chromatography and thin layer chromatography. The structures of isolated compounds were elucidated through spectroscopic analysis including 1D-NMR (1H, 13C and DEPT), 2D-NMR (1H-1H COSY, HSQC/HMQC and HMBC), IR, UV, MS (GCMS); also by comparison with previous literature data. Isolation study on the leaves of K. singapurensis has yielded lupeol 147, mixture of stigmasterol 148 and - sitosterol 149, lupeol ester 150, and a mixture of lupeol 147, -amyrin 151 and - amyrin 152. Compounds -amyrin 152, -amyrenone 153, -amyrin acetate 154 and lupeol acetate 155 were successfully isolated from the bark of K. singapurensis. These triterpenoids were isolated for the first time from this species. Compounds 147, 150, 152, 153, 154 and 155 were exhibited cytotoxic effects against MCF-7 in the range of IC50 15.5 - 26.0 g/mL. All isolated compounds showed weak antioxidant activity (IC50 >500 g/mL) and were not active against Bacillus cereus. Kopsia singapurensis was one of the threatened species however there is no publication on terpene isolated from this species. Hence, study on the chemical constituents of K. singapurensis has been embarked to discover new active compound for pharmaceutical industries. 2015 thesis https://ir.upsi.edu.my/detailsg.php?det=1668 https://ir.upsi.edu.my/detailsg.php?det=1668 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A
institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	QD Chemistry
spellingShingle	QD Chemistry Lee, Yean Shan Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)
description	This study was aimed to extract and identify chemical compounds isolated from the leaves and barks of Kopsia singapurensis. The isolated compounds were evaluated for cytotoxic (MCF-7), antibacterial (Bacillus cereus) and antioxidant activity (DPPH). The isolation process was carried out using chromatographic techniques such as column chromatography and thin layer chromatography. The structures of isolated compounds were elucidated through spectroscopic analysis including 1D-NMR (1H, 13C and DEPT), 2D-NMR (1H-1H COSY, HSQC/HMQC and HMBC), IR, UV, MS (GCMS); also by comparison with previous literature data. Isolation study on the leaves of K. singapurensis has yielded lupeol 147, mixture of stigmasterol 148 and - sitosterol 149, lupeol ester 150, and a mixture of lupeol 147, -amyrin 151 and - amyrin 152. Compounds -amyrin 152, -amyrenone 153, -amyrin acetate 154 and lupeol acetate 155 were successfully isolated from the bark of K. singapurensis. These triterpenoids were isolated for the first time from this species. Compounds 147, 150, 152, 153, 154 and 155 were exhibited cytotoxic effects against MCF-7 in the range of IC50 15.5 - 26.0 g/mL. All isolated compounds showed weak antioxidant activity (IC50 >500 g/mL) and were not active against Bacillus cereus. Kopsia singapurensis was one of the threatened species however there is no publication on terpene isolated from this species. Hence, study on the chemical constituents of K. singapurensis has been embarked to discover new active compound for pharmaceutical industries.
format	thesis
qualification_name
qualification_level	Master's degree
author	Lee, Yean Shan
author_facet	Lee, Yean Shan
author_sort	Lee, Yean Shan
title	Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)
title_short	Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)
title_full	Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)
title_fullStr	Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)
title_full_unstemmed	Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)
title_sort	identification of triterpene from the leaves and bark of kopsia singapurensis ridl. (selada) (ir)
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Sains dan Matematik
publishDate	2015
url	https://ir.upsi.edu.my/detailsg.php?det=1668
_version_	1747832987062370304

Identification of triterpene from the leaves and bark of kopsia singapurensis Ridl. (SELADA) (IR)

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