Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)

Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)

The purpose of this study is to categorise the trapped rice field rat, Rattus argentiventer in Pinang Tunggal paddy field into their respective haplotype groups and to investigate if mutation occurs in the VKORC1 gene of Rattus argentiventer that was exposed to anticoagulant rodenticide. The study a...

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Main Author: Muhammad Shahredza Sharuddin
Format: thesis
Language:eng
Published: 2016
Subjects:
SB Plant culture
Online Access:https://ir.upsi.edu.my/detailsg.php?det=1687
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spelling oai:ir.upsi.edu.my:16872020-02-27 Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR) 2016 Muhammad Shahredza Sharuddin SB Plant culture The purpose of this study is to categorise the trapped rice field rat, Rattus argentiventer in Pinang Tunggal paddy field into their respective haplotype groups and to investigate if mutation occurs in the VKORC1 gene of Rattus argentiventer that was exposed to anticoagulant rodenticide. The study also aims to determine the warfarin residue level in the liver of the rat samples. Prior to haplotype analysis, the DNA of the captured rodents was extracted and the HVR1 of mitochondrial DNA was amplified. Furthermore, Restriction Fragment Length Polymorphism (RFLP) analysis was used in order to investigate the mutation of VKORC1 gene. The rats livers were also being used to analyse the residue level of warfarin intake using the High Performance Liquid Chromatography (HPLC). The results showed that two haplotype groups among the 58 samples were detected and no mutation was found in the entire trapped rodent. The results also demonstrated that the amount of warfarin level in the liver was at 1.421 min of retention time. This is relatively low in concentration, which in turn assured that there was no mutation detected in the earlier RFLP analysis. In conclusion, low haplotype numbers and no mutation of VKORC1 gene was detected in the trapped Rattus argentiventer. Implication of this research is that it prepares as a reference for future studies on molecular profile of Rattus argentiventer in Pinang Tunggal paddy field, Pulau Pinang. 2016 thesis https://ir.upsi.edu.my/detailsg.php?det=1687 https://ir.upsi.edu.my/detailsg.php?det=1687 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A
institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language eng
topic SB Plant culture
spellingShingle SB Plant culture
Muhammad Shahredza Sharuddin
Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)
description The purpose of this study is to categorise the trapped rice field rat, Rattus argentiventer in Pinang Tunggal paddy field into their respective haplotype groups and to investigate if mutation occurs in the VKORC1 gene of Rattus argentiventer that was exposed to anticoagulant rodenticide. The study also aims to determine the warfarin residue level in the liver of the rat samples. Prior to haplotype analysis, the DNA of the captured rodents was extracted and the HVR1 of mitochondrial DNA was amplified. Furthermore, Restriction Fragment Length Polymorphism (RFLP) analysis was used in order to investigate the mutation of VKORC1 gene. The rats livers were also being used to analyse the residue level of warfarin intake using the High Performance Liquid Chromatography (HPLC). The results showed that two haplotype groups among the 58 samples were detected and no mutation was found in the entire trapped rodent. The results also demonstrated that the amount of warfarin level in the liver was at 1.421 min of retention time. This is relatively low in concentration, which in turn assured that there was no mutation detected in the earlier RFLP analysis. In conclusion, low haplotype numbers and no mutation of VKORC1 gene was detected in the trapped Rattus argentiventer. Implication of this research is that it prepares as a reference for future studies on molecular profile of Rattus argentiventer in Pinang Tunggal paddy field, Pulau Pinang.
format thesis
qualification_name
qualification_level Master's degree
author Muhammad Shahredza Sharuddin
author_facet Muhammad Shahredza Sharuddin
author_sort Muhammad Shahredza Sharuddin
title Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)
title_short Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)
title_full Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)
title_fullStr Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)
title_full_unstemmed Analysis of DNA polymorphism within Rattus argentiventer (Robinson & Kloss) population in Pinang Tunggal paddy field, Pulau Pinang (IR)
title_sort analysis of dna polymorphism within rattus argentiventer (robinson & kloss) population in pinang tunggal paddy field, pulau pinang (ir)
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Sains dan Matematik
publishDate 2016
url https://ir.upsi.edu.my/detailsg.php?det=1687
_version_ 1747832988296544256

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