Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)

The objectives of this study are to synthesize novel chitosan-hydroxyapatite composites doped with strontium, magnesium and zinc via in situ co-precipitation method, to investigate the bioactivity, cytotoxicity and antimicrobial properties of the materials. Physicochemical properties of the products...

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Main Author:	Ismaila Abdullahi
Format:	thesis
Language:	eng
Published:	2016
Subjects:	QC Physics
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=341
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spelling	oai:ir.upsi.edu.my:3412020-02-27 Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR) 2016 Ismaila Abdullahi QC Physics The objectives of this study are to synthesize novel chitosan-hydroxyapatite composites doped with strontium, magnesium and zinc via in situ co-precipitation method, to investigate the bioactivity, cytotoxicity and antimicrobial properties of the materials. Physicochemical properties of the products obtained were analysed by X-ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential thermal analysis (DTA), field emission scanning electron microscopy (FESEM) and energy dispersive X-ray spectroscopy (EDX). The calcium release from the samples in simulated body fluid (SBF) were measured using atomic absorption spectroscopy (AAS). The synthesized composite materials were subjected to bioactivity studies in SBF. In vitro cytotoxicity studies were performed on the novel composite materials obtained. Antimicrobial activity investigations against E. coli were carried out on all the composite samples. Characterization of the composite samples revealed that three novel composites, viz; chitosan-hydroxyapatite composite doped with strontium, chitosan-hydroxyapatite composites doped with magnesium and chitosan-hydroxyapatite composite doped with zinc were successfully synthesized. All the composites obtained showed good bioactivity in SBF. The morphologies of the composites obtained by FESEM analysis revealed that they have good apatite forming ability in SBF. The calcium/phosphorous ratio (Ca/P) of the composites calculated after soaking in SBF have values that are comparable to that of natural apatite. Chitosan-hydroxyapatite composites doped with strontium and zinc were found to be non-cytotoxic against the cell culture used. Chitosan-hydroxyapatite composite doped with magnesium was found to be mildly cytotoxic. Chitosan-hydroxyapatite composites doped with strontium and magnesium show no antimicrobial effects against the E. coli bacteria. Chitosan-hydroxyapatite composite doped with zinc show substantial antibacterial effect against E. coli. The novel composites obtained thus far can be used potentially for bone grafting, bone filler and template for bone tissue engineering. 2016 thesis https://ir.upsi.edu.my/detailsg.php?det=341 https://ir.upsi.edu.my/detailsg.php?det=341 text eng closedAccess Doctoral Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A
institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	QC Physics
spellingShingle	QC Physics Ismaila Abdullahi Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)
description	The objectives of this study are to synthesize novel chitosan-hydroxyapatite composites doped with strontium, magnesium and zinc via in situ co-precipitation method, to investigate the bioactivity, cytotoxicity and antimicrobial properties of the materials. Physicochemical properties of the products obtained were analysed by X-ray powder diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential thermal analysis (DTA), field emission scanning electron microscopy (FESEM) and energy dispersive X-ray spectroscopy (EDX). The calcium release from the samples in simulated body fluid (SBF) were measured using atomic absorption spectroscopy (AAS). The synthesized composite materials were subjected to bioactivity studies in SBF. In vitro cytotoxicity studies were performed on the novel composite materials obtained. Antimicrobial activity investigations against E. coli were carried out on all the composite samples. Characterization of the composite samples revealed that three novel composites, viz; chitosan-hydroxyapatite composite doped with strontium, chitosan-hydroxyapatite composites doped with magnesium and chitosan-hydroxyapatite composite doped with zinc were successfully synthesized. All the composites obtained showed good bioactivity in SBF. The morphologies of the composites obtained by FESEM analysis revealed that they have good apatite forming ability in SBF. The calcium/phosphorous ratio (Ca/P) of the composites calculated after soaking in SBF have values that are comparable to that of natural apatite. Chitosan-hydroxyapatite composites doped with strontium and zinc were found to be non-cytotoxic against the cell culture used. Chitosan-hydroxyapatite composite doped with magnesium was found to be mildly cytotoxic. Chitosan-hydroxyapatite composites doped with strontium and magnesium show no antimicrobial effects against the E. coli bacteria. Chitosan-hydroxyapatite composite doped with zinc show substantial antibacterial effect against E. coli. The novel composites obtained thus far can be used potentially for bone grafting, bone filler and template for bone tissue engineering.
format	thesis
qualification_name
qualification_level	Doctorate
author	Ismaila Abdullahi
author_facet	Ismaila Abdullahi
author_sort	Ismaila Abdullahi
title	Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)
title_short	Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)
title_full	Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)
title_fullStr	Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)
title_full_unstemmed	Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)
title_sort	synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (ir)
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Sains dan Matematik
publishDate	2016
url	https://ir.upsi.edu.my/detailsg.php?det=341
_version_	1747832876196429824

Synthesis, characterization and in vitro response of chitosan hydroxyapatite composites doped with strontium, magnesium and zinc (IR)

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