In vitro regeneration and acclimatization of Carica papaya L. (IR)

The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Random...

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Main Author: Muhammad Ichsan M. Nasir
Format: thesis
Language:eng
Published: 2018
Subjects:
Online Access:https://ir.upsi.edu.my/detailsg.php?det=3811
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spelling oai:ir.upsi.edu.my:38112020-02-27 In vitro regeneration and acclimatization of Carica papaya L. (IR) 2018 Muhammad Ichsan M. Nasir SB Plant culture The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Randomized Design (CRD). For in vitro plant regeneration study, Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) concentrations and combinations were used ranging from 0.0 mg/L - 2.0 mg/L. The finding of this research shows that optimum callus induction was obtained when root explants were cultured on Murashige and Skoog (MS) medium fortified with 1.5 mg/L BAP and 0.5 mg/L NAA. Friable cream callus was produced. Complete plant regeneration was successfully achieved when root, stem, leaf and petiol aseptic explants were cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 1.0 mg/L BAP was found to be the optimum medium and stem explant was the most responsive explant producing 28.73 6.03 shoots per explant. MS medium (with 1.5 mg/L NAA) was most optimum for root induction. Meanwhile, synthetic seeds of Carica papaya L. were produced when micro shoots were encapsulated with 4.0% sodium alginate solution added with 1.5 mg/L BAP and 1.0 mg/L NAA. Synthetic seeds germination rate was 2.56 0.43 shoots per explant. Finally, acclimatization of Carica papaya L. was accomplished when plantlets were transferred to the combination of black and red soil (2:1) with 86.67% survival rate. In conclusion, the research showed that in vitro propagation of Carica papaya L. through tissue culture system was successfully achieved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of papaya plants, therefore could increase the crop production. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=3811 https://ir.upsi.edu.my/detailsg.php?det=3811 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional N/A
institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language eng
topic SB Plant culture
spellingShingle SB Plant culture
Muhammad Ichsan M. Nasir
In vitro regeneration and acclimatization of Carica papaya L. (IR)
description The purpose of this reseach is to study in vitro organogenesis of Carica papaya L. through tissue culture system. In vitro plant regeneration, production of synthetic seeds and acclimatization of plantlets were investigated. The experimental design that was used in this research is Completely Randomized Design (CRD). For in vitro plant regeneration study, Benzylaminopurine (BAP) and Naphthalene Acetic Acid (NAA) concentrations and combinations were used ranging from 0.0 mg/L - 2.0 mg/L. The finding of this research shows that optimum callus induction was obtained when root explants were cultured on Murashige and Skoog (MS) medium fortified with 1.5 mg/L BAP and 0.5 mg/L NAA. Friable cream callus was produced. Complete plant regeneration was successfully achieved when root, stem, leaf and petiol aseptic explants were cultured on MS medium supplemented with various combinations of plant growth regulators. MS medium supplemented with 1.0 mg/L BAP was found to be the optimum medium and stem explant was the most responsive explant producing 28.73 6.03 shoots per explant. MS medium (with 1.5 mg/L NAA) was most optimum for root induction. Meanwhile, synthetic seeds of Carica papaya L. were produced when micro shoots were encapsulated with 4.0% sodium alginate solution added with 1.5 mg/L BAP and 1.0 mg/L NAA. Synthetic seeds germination rate was 2.56 0.43 shoots per explant. Finally, acclimatization of Carica papaya L. was accomplished when plantlets were transferred to the combination of black and red soil (2:1) with 86.67% survival rate. In conclusion, the research showed that in vitro propagation of Carica papaya L. through tissue culture system was successfully achieved. It proves that plant tissue culture technology could be an alternative solution to achieve high quality of papaya plants, therefore could increase the crop production.
format thesis
qualification_name
qualification_level Master's degree
author Muhammad Ichsan M. Nasir
author_facet Muhammad Ichsan M. Nasir
author_sort Muhammad Ichsan M. Nasir
title In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_short In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_full In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_fullStr In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_full_unstemmed In vitro regeneration and acclimatization of Carica papaya L. (IR)
title_sort in vitro regeneration and acclimatization of carica papaya l. (ir)
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Teknikal dan Vokasional
publishDate 2018
url https://ir.upsi.edu.my/detailsg.php?det=3811
_version_ 1747833102856617984