Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)

Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)

The purpose of this study was to investigate the chemical compounds from the calyces of Hibiscus sabdariffa Linn. and their α-glucosidase inhibitory activity. Samples were collected from Balik Pulau, Pulau Pinang. The total of three kilogram of samples were air dried at room temperature, g...

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Main Author: Nadzirah Mohd Jauah
Format: thesis
Language:zsm
Published: 2018
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QP Physiology
Online Access:https://ir.upsi.edu.my/detailsg.php?det=3946
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spelling oai:ir.upsi.edu.my:39462020-02-27 Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR) 2018 Nadzirah Mohd Jauah QP Physiology The purpose of this study was to investigate the chemical compounds from the calyces of Hibiscus sabdariffa Linn. and their α-glucosidase inhibitory activity. Samples were collected from Balik Pulau, Pulau Pinang. The total of three kilogram of samples were air dried at room temperature, grinded and serially extracted by solid liquid extraction technique using hexane, dichloromethane, methanol, ethanol and water. Compounds were isolated and purified by several chromatographic techniques. Their structures were elucidated with spectroscopic methods including Nuclear Magnetic Resonance (1D-NMR and 2D-NMR), Liquid Chromatography-Mass (LCMS) spectrometry, infrared (IR) spectroscopy, ultraviolet (UV) spectroscopy and compared with data reported in the literature. The α-glucosidase inhibitory activity on the crude extracts and pure compounds was conducted using α -glucosidase inhibitory assay. The finding of this phytochemical study has led to the isolation of seven pure compounds namely squalene, triglyceride fatty acid, ethyl stearate, 4-ethoxy-3-((ethoxycarbonyl)oxy)-4- oxobutanoic acid, 2-(1-ethoxy-4-methoxy-1,4-dioxobutan-2-yl)-1,5-dimethyl-3-(2- ethoxy-2-oxoethyl)-4-(2-methoxy-2-oxoethyl-3-(methoxycarbonyl)oxy)pentane-1,2,5 -tricarboxylate, 2-(2-ethoxy-2-oxoethyl)-1,5-dimethyl-3,4-bis-(2-methoxy-2-oxoethyl) -3-((methoxycarbonyl)oxy)pentane-1,2,5-tricarboxylate, 7-((1,5-dimethoxy-1,4-dioxo pentan-3yl)oxy)-3,11-bis(ethoxycarbonyl)-7-(3-methoxy-1-((methoxycarbonyl)oxy)-3 -oxopropyl)-5,9-dioxo-4,6,8,10-tetraoxatridecane-1,8-dioic acid along with two mixture compounds; mixture of stigmasterol with β-sitosterol and mixture of 3- hydroxy-4-oxopentanal with 5-hydroxy methylfurfural. The methanol crude extract showed the highest activity among other crude extracts towards α -glucosidase compared to acarbose and quercetin. In addition, the selected isolated compounds were not active against the α-glucosidase inhibitory activity. As a conclusion, inactive compounds which isolated from the calyces of Hibiscus sabdariffa Linn. did not show any α-glucosidase inhibitory activity. Implication of this study is it can be used as future references for phytochemical studies of this species to discover possible potential new drugs in pharmaceutical. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=3946 https://ir.upsi.edu.my/detailsg.php?det=3946 text zsm closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik N/A
institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language zsm
topic QP Physiology
spellingShingle QP Physiology
Nadzirah Mohd Jauah
Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)
description The purpose of this study was to investigate the chemical compounds from the calyces of Hibiscus sabdariffa Linn. and their α-glucosidase inhibitory activity. Samples were collected from Balik Pulau, Pulau Pinang. The total of three kilogram of samples were air dried at room temperature, grinded and serially extracted by solid liquid extraction technique using hexane, dichloromethane, methanol, ethanol and water. Compounds were isolated and purified by several chromatographic techniques. Their structures were elucidated with spectroscopic methods including Nuclear Magnetic Resonance (1D-NMR and 2D-NMR), Liquid Chromatography-Mass (LCMS) spectrometry, infrared (IR) spectroscopy, ultraviolet (UV) spectroscopy and compared with data reported in the literature. The α-glucosidase inhibitory activity on the crude extracts and pure compounds was conducted using α -glucosidase inhibitory assay. The finding of this phytochemical study has led to the isolation of seven pure compounds namely squalene, triglyceride fatty acid, ethyl stearate, 4-ethoxy-3-((ethoxycarbonyl)oxy)-4- oxobutanoic acid, 2-(1-ethoxy-4-methoxy-1,4-dioxobutan-2-yl)-1,5-dimethyl-3-(2- ethoxy-2-oxoethyl)-4-(2-methoxy-2-oxoethyl-3-(methoxycarbonyl)oxy)pentane-1,2,5 -tricarboxylate, 2-(2-ethoxy-2-oxoethyl)-1,5-dimethyl-3,4-bis-(2-methoxy-2-oxoethyl) -3-((methoxycarbonyl)oxy)pentane-1,2,5-tricarboxylate, 7-((1,5-dimethoxy-1,4-dioxo pentan-3yl)oxy)-3,11-bis(ethoxycarbonyl)-7-(3-methoxy-1-((methoxycarbonyl)oxy)-3 -oxopropyl)-5,9-dioxo-4,6,8,10-tetraoxatridecane-1,8-dioic acid along with two mixture compounds; mixture of stigmasterol with β-sitosterol and mixture of 3- hydroxy-4-oxopentanal with 5-hydroxy methylfurfural. The methanol crude extract showed the highest activity among other crude extracts towards α -glucosidase compared to acarbose and quercetin. In addition, the selected isolated compounds were not active against the α-glucosidase inhibitory activity. As a conclusion, inactive compounds which isolated from the calyces of Hibiscus sabdariffa Linn. did not show any α-glucosidase inhibitory activity. Implication of this study is it can be used as future references for phytochemical studies of this species to discover possible potential new drugs in pharmaceutical.
format thesis
qualification_name
qualification_level Master's degree
author Nadzirah Mohd Jauah
author_facet Nadzirah Mohd Jauah
author_sort Nadzirah Mohd Jauah
title Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)
title_short Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)
title_full Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)
title_fullStr Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)
title_full_unstemmed Chemical constituents from the calyces of Hibiscus Sabdariffa Linn and its a-Glucosidase inhibitory activity (IR)
title_sort chemical constituents from the calyces of hibiscus sabdariffa linn and its a-glucosidase inhibitory activity (ir)
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Sains dan Matematik
publishDate 2018
url https://ir.upsi.edu.my/detailsg.php?det=3946
_version_ 1747833114642612224

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