The development of optical stiffness calibration software base on equipartition theorem, boltzman statistics and power spectrum density
This study aimed to develop an optical stiffness calibration system using threemethods. The used methods were Boltzmann statistics (BS), equipartition theorem (ET) and powerspectrum density (PSD) analysis. This study consisted of two phases; system development phase andtesting phase. System developm...
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| Format: | thesis |
| Language: | eng |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=5008 |
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| Summary: | This study aimed to develop an optical stiffness calibration system using threemethods. The used methods were Boltzmann statistics (BS), equipartition theorem (ET) and powerspectrum density (PSD) analysis. This study consisted of two phases; system development phase andtesting phase. System development phase involved hardware and software components. Testing phaseinvolved actual experimental optical stiffness calibration. The finding of the study was acalibration system consisting hardware and software parts. The hardware part was an opticaltweezers that was equipped with quadrant photodiode (QPD) and piezostage. The software part was acustom made software which was constructed on LabVIEW 2012 platform, namely OSCal. This studyshowed the conversion factor (used in ET and BS methods) and optical stiffness depends on trappinglaser power. In conclusion, this study successfully developed the aimed system with comparableoptical stiffness between each method and other reference studies. The implication of this study isthat the time spent for calibration for optical tweezers procedure can be shortened so that otherresearcher can focus more on the intended applications of the optical tweezers. |
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