Development of fish scale-collagen cream (CoC) from water-in-virgin coconut oil emulsion medium for topical application
This study aimed to develop and characterise the fish scale-collagen cream (CoC) fortopical application. Hydrolysed collagen (HC) was extracted from Tilapia fish scales using acombination of hydrothermal extraction and enzymatic hydrolysis. This HC (molecular weight, Mw 1 kDa) was incorporated in wa...
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| Format: | thesis |
| Language: | eng |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=5365 |
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| Summary: | This study aimed to develop and characterise the fish scale-collagen cream (CoC) fortopical application. Hydrolysed collagen (HC) was extracted from Tilapia fish scales using acombination of hydrothermal extraction and enzymatic hydrolysis. This HC (molecular weight, Mw 1 kDa) was incorporated in water-in-oil (w/o) emulsion of Span 60:Tween 60/water/virgin coconut oil(VCO) system at water volume fractions (w) of 0.74, 0.83, and 0.94 via high shear homogenisationtechnique. The developed CoC were then characterised by optical polarising microscope (OPM), fieldemission scanning electron microscopy (FESEM), particle size analyser, differential scanningcalorimetry (DSC), and rheometer. Up to 20 wt% HC has been successfully loaded in all emulsionsystems and the optimum loading achieved at HC content as low as 5 wt%. The best CoC systemwith great physical characteristic, fine texture (305 nm) and uniformly disperse in VCO phasewas produced by emulsion at the lowest w (0.94). The developed CoC has been foundto be thermally stable with Tc and Tm range between -20 to -30 ? and 10 to 20 ?,respectively. The rheological properties of CoC demonstrated shear thinning behaviour andthus greatly meet the needs of topical applications. The permeation study of ex vivorat skin showed that HC in emulsion droplets (CoC system) easily pass through theskin. Cytotoxicity study through in vitro fibroblast cells and in vivo toxicity to the miceshowed that HC at 25 and 50 mg/kg body weight did not give chronic toxic to the mice. Inconclusion, CoC from water-in-VCO emulsion system was succesfully developed. As an implication,the developed CoC is potentially used as a carrier for HC in topical application. |
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