In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L.
The purpose of this study was to examine the ability of seed germination Ocimumbasilicum L. to germinate through tissue culture, the rate of time difference in seedgermination in traditional or modern technology. In addition, callus formation was alsocarried out in the study to see the rate of cell...
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| Format: | thesis |
| Language: | eng |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=5488 |
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| Summary: | The purpose of this study was to examine the ability of seed germination Ocimumbasilicum L. to germinate through tissue culture, the rate of time difference in seedgermination in traditional or modern technology. In addition, callus formation was alsocarried out in the study to see the rate of cell differentiation at the exploratory part. Atthe end of the study, it was also tested by acclimatization of the explant to green house.The complete regeneration of the Ocimum basilicum L. plant and callus production wassuccessfully produced through the tissue culture technique. The part of leaves, stemsand roots of 8-week aseptic seedlings were used in this experiment. All cultures arestored at a temperature of 25 1C and illuminated with fluorescent light for 16 hoursof light, 8 hours dark. Plant acclimatization is done on three different soil types, namelygarden soil, coconut powder and vermiculation. After completion, all data was recordedand analyzed using ANNOVA. Using MS medium (Murashige and Skoog, 1962) rootexplants were found to be very responsive for 0.5 mg / l BAP and 1.0 mg / l NAAconcentration to produce maximum callus weight, and The stem explant for aconcentration of 0.5 mg / l BAP with 1.5 mg / l of NAA will produce double shoots.Growth of seedlings are most optimum when regenerated from stem explants areacclimated in a garden with the survival rate of 88 0.8%. As the conclusion, thismethod of tissue culture is the best method in producing new generation of Ocimumbasilicum L. and its features are preserved. The implications of this study suggest thatthis tissue culture method can help to regenerate this species from extinction and thebenefits of its use can continue to apply. |
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