In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L.
The purpose of this study was to examine the ability of seed germination Ocimumbasilicum L. to germinate through tissue culture, the rate of time difference in seedgermination in traditional or modern technology. In addition, callus formation was alsocarried out in the study to see the rate of cell...
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SD Forestry Siti Zubaidah Lood In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. |
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The purpose of this study was to examine the ability of seed germination Ocimumbasilicum L. to germinate through tissue culture, the rate of time difference in seedgermination in traditional or modern technology. In addition, callus formation was alsocarried out in the study to see the rate of cell differentiation at the exploratory part. Atthe end of the study, it was also tested by acclimatization of the explant to green house.The complete regeneration of the Ocimum basilicum L. plant and callus production wassuccessfully produced through the tissue culture technique. The part of leaves, stemsand roots of 8-week aseptic seedlings were used in this experiment. All cultures arestored at a temperature of 25 1C and illuminated with fluorescent light for 16 hoursof light, 8 hours dark. Plant acclimatization is done on three different soil types, namelygarden soil, coconut powder and vermiculation. After completion, all data was recordedand analyzed using ANNOVA. Using MS medium (Murashige and Skoog, 1962) rootexplants were found to be very responsive for 0.5 mg / l BAP and 1.0 mg / l NAAconcentration to produce maximum callus weight, and The stem explant for aconcentration of 0.5 mg / l BAP with 1.5 mg / l of NAA will produce double shoots.Growth of seedlings are most optimum when regenerated from stem explants areacclimated in a garden with the survival rate of 88 0.8%. As the conclusion, thismethod of tissue culture is the best method in producing new generation of Ocimumbasilicum L. and its features are preserved. The implications of this study suggest thatthis tissue culture method can help to regenerate this species from extinction and thebenefits of its use can continue to apply. |
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Siti Zubaidah Lood |
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Siti Zubaidah Lood |
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Siti Zubaidah Lood |
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In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. |
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In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. |
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In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. |
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In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. |
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In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. |
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in vitro morphogenic response from different explants of sweet basils, ocimum basillicum l. |
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Universiti Pendidikan Sultan Idris |
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Fakulti Teknikal dan Vokasional |
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oai:ir.upsi.edu.my:54882021-01-12 In vitro morphogenic response from different explants of sweet basils, Ocimum basillicum L. 2018 Siti Zubaidah Lood SD Forestry The purpose of this study was to examine the ability of seed germination Ocimumbasilicum L. to germinate through tissue culture, the rate of time difference in seedgermination in traditional or modern technology. In addition, callus formation was alsocarried out in the study to see the rate of cell differentiation at the exploratory part. Atthe end of the study, it was also tested by acclimatization of the explant to green house.The complete regeneration of the Ocimum basilicum L. plant and callus production wassuccessfully produced through the tissue culture technique. The part of leaves, stemsand roots of 8-week aseptic seedlings were used in this experiment. All cultures arestored at a temperature of 25 1C and illuminated with fluorescent light for 16 hoursof light, 8 hours dark. Plant acclimatization is done on three different soil types, namelygarden soil, coconut powder and vermiculation. After completion, all data was recordedand analyzed using ANNOVA. Using MS medium (Murashige and Skoog, 1962) rootexplants were found to be very responsive for 0.5 mg / l BAP and 1.0 mg / l NAAconcentration to produce maximum callus weight, and The stem explant for aconcentration of 0.5 mg / l BAP with 1.5 mg / l of NAA will produce double shoots.Growth of seedlings are most optimum when regenerated from stem explants areacclimated in a garden with the survival rate of 88 0.8%. As the conclusion, thismethod of tissue culture is the best method in producing new generation of Ocimumbasilicum L. and its features are preserved. The implications of this study suggest thatthis tissue culture method can help to regenerate this species from extinction and thebenefits of its use can continue to apply. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=5488 https://ir.upsi.edu.my/detailsg.php?det=5488 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional Abobkar I. M. Saad & Ahmad M. Elshahed (2012), Recent Advences in Plant in vitroCulture, Chapter 2 : Plant Tissue Culture Media (pp. 22-40), INTECHAcheapong E. (1982). Multiplication and Conservation of Cocoyam (Xarthosoma sajiltifoljum)germplasm by application of tissue culture methods Ph.D Thesis. University of Burmingham, UKAladele S. E. & Kuta D. (2008). Environmental and genotypic effects on the rowth rate of invitro cassava plantlet (Manihot esceulenta) African Journal of Biotechnology Vol. 7 (4), pp.381-385.Aloni, R. (1980). Role of auxin and sucrose in the differentiation of sieve and tracheary elementsin plant tissue cultures. Planta. 150: 255 263.Arditti, J., & Ernst, R. (1993). Micropropagation of Orchids. John Wiley & Sons, Inc, Canada,pp:1-665Bapat, V.A., Mhatre, M. and Rao, P.S. (1987). Propagation of Morus indica (mulberry) byencapsulated shoot buds. Plant Cell Rep. 6: 393-395.Bhojawi, S.S., & Razdan, M.K. (1996). Plant Tissue Culture: Theory and Practise, a Revised Edition,Elsever, Amsterdam, pp:39-62Bhojwani, S.S. & Razdan, M.K. (1992). Plant tissue culture: Theory and practice.Elsivier, Amsterdam, London, New York, Tokyo.Biren N. S., Avinash K. S. (2010), Pharmacognostic Studies of The Lagenaria siceraria(Molina) Standley, Gujerat, IndiaButenko, R.G. (1968). Plant tissue culture and plant morphogenesis. Translated from Russian.Israelprogram for Scientific Translation, Jerusalem.Capuano C, Piccioni E, Standardi A (1998). Effect of different treatments on theconversion of M.26 apple rootstock synthetic seeds obtained from encapsulated apical and axillarymicropropagated buds. J. Hort. Sci. 73:299-305.Carew,D.P., & Staba, E.J. (1965). Plant tissue culture; its fundamentals, application andrelationship to medicinal plant studies. Lloydia. 28: 1-26.Cassells, A.C.(1991). Problems in tissue culture: Culture contamination. In:Micropropagation Technology tind Application. P.C. Debergh and R.H. Zimmerm, cds.Kluwer Academic Publishers. Dordrecht, Netherlands. pp. 31-44.Chaleff, R. S., and P. S. Carlson. (1974). Somatic cell genetics of higher plants. Ann.Rev. Genet. 8: 267-278.Chan , P.Y., Fujita, S., Kohno, K., Ashrafuzzaman, M.D., Nakamura, N. and Hayashi,N. (2000). Purification and characterization of polyphenol oxidase from banana (Musa sapietum L.) pulp. Journal of Agricultural and Food Chemistry 48: 2732-2735.Cocking, E. C. 1972. Plant cell protoplasts-isolation and development. Ann. Rev. Plant Physiol. 23:29-50.Conger, B. V. (1980). Cloning Agricultural Plants via In-vitro Techniques, CRC Press Inc, BocaRaton, Florida, pp; 1-50Dalmacio I. F. (1992). Biotechnology for sustainable Agriculture National Institute ofBiotechnology and Applied Microbiology, University of the Philippines.Dalziel,J. M. 1937. The useful plants of West Tropical Africa. Whitefriars Press, London, 612 pp.Daniel, R.L. (1998). The many dimension of plant tissue culture research. Webmaster of AggieHorticulture Publications, pp.201-210.de Fossard R.(1976) Tissue culture for plant propagation. Armidale: University of New England.Driver, J.A., & Kuniyuki, A.H. (1984) In vitro propagation of paradox walnut rootstock.Hotr. Sci., 19: 507-709.Encyclopedia of Life (2009), Introduction. http://eol.org/pages/281/overviewEnjalric, F., Carron, M.P., Lardet, L.(1988). Contamination of Primary Cultures inTropical Areas: The Case of Hevea Brasiliensis. In:Bacterial and Bacteria-like Contaminants ofPlant Tissue Cultures. ISHS Acta Horticulturae. 225:Fabijan D, Taylor JS & Reid DM (1981) Adventitious rooting in hypocotyls of sunflower (Helianthusannuus) seedlings II. Action of gibberellins, cytokinins, auxins and ethylene. PhysiologiaPlantarum 53: 589?597.Food and Agriculture Organization (FAO). (2005). Statistics Database (Agriculture Data)2001. On internet http:/apps.fao.org.Frusciante, L., Barone, A.,Carputo,D. Ercolano, M.R., Della Rocca, F., & Esposito,S. (2000). Evaluation and use of Plant Biodiversity for Food and Pharmaceutical. Fitoterpia. 71 :66 S72.Gamborg, O., Miller, R. & Ojima, K. 1968). Nutrient requirement suspensions cultures of soybeanroot cells. Experimental Cell Research. 50(1):151-158.Gautheret, R. J. (1934). Culture du tissus cambial. Comptes Rendus Hebdomadaires des Sances del'Acadmie des Sciences, 198, 21952196.Gautheret, R. J. (1935). Recherches sur la culture des tissus vgtaux. Ph.D. Thesis,ParisGautheret, R. J. (1939). Sur la possibilit de raliser la culture indfinie des tissus detubercules de carotte. Comptes Rendus Hebdomadaires des Sances de l'Acadmie desSciences, 208, 118120.Gbile Z.O.(1998). Collection, Conservation and Utilization of medicinal plants. In RobertPA, & Janice EA (eds.). Conservation of Plant Genes III: Conservation and Utilization of AfricanPlants. Missouri Botanical Gardens Press. pp. 163-17Georges, D., Chenieux, J.C. & Ochatt. S.J. (1993). Plant regeneration from aged-callus of the woodyornamental species Lonicera japonica cv. "Hall's prolific". Plant Cell Reports. 13:91-94. Growth ofmonocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199-204.Han, J-S., Oh, D-G., Mok, I-G., Park, H-G., Kim, C. K., (2004), Efficient plantregeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). PlantCell Rep., 23:291-296 Improvement of cucurbits; In: Biology and utilization of the cucurbitaceae;pp. 367?381 eds. D M Bates, R W Robinson & C Jeffrey(Ithaca, London: Cornell Univ. Press).Hartmann, H.T., Kester, D.E. & Davies, F.T. (1990). Plant propagation: principles and practices.Englewood Cliffs, NJ: Prentice Hall.IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, SwitzerlandKanchanapoom, K., Jantaro, S. & Rakchad, D. (2001). Isolation and fusion ofprotoplasts from mesophyll cells of Dendrobium pompadour. Science Asia. 27:29-34.Kane, M. (2003). Bacterial and fungal indexing of tissue cultures. http://www.hos.ufl.edu/Nwe, N., T., & Tamur, H. (2010). Production of Fungal Chitosan by Enzymatic Method and Applicationsin Plant Tissue Culture and Tissue Engineering: 11 Years of Our Progress, Present Situation and Future Prospects. Biopolymers. doi:10.5772/10261Kasha, K. J. (1974). Haploids in Higher Plants. University of Guelph, Guelph.Katare, C., Agrawal, S., Jain, M., Rani, S., Saxena, S., Bisen, P.S., Prasad, G.B.K.S. (2011).Lagenaria siceraria : A Potential Source of Anti-Hyperlipidermic and Other Pharmacological Agents.Current Nutrition & Food Science Vol. 7, No 3, pp 1- 9Krikorian AD (1995) Hormones in tissue culture and micropropagation. In: Plant hormones: physiology, biochemistry and molecular biology (P.J. Davies, ed.). Kluwer Academic Publishers, Dordrecht, p. 774?796.Lee, J.M. (1994). Cultivation of grafted vegetables I. Current status, grafting methods, andbenefits. HortSci. 29: 235-239.Lee, J.M. (2003). Advances in vegetable grafting. Chronica Horticulturae. 43:13-19.Lloyd, G. & McCown, B. (1980) Commercially-feasible micropropagation ofmountainlaurel, Kalmia latifolia, by use of shoot-tip culture. Intern. Plant Prop. Soc. Proc. 30:421427.Mendi, Y.Y., Ipek, M., Buzkan, N., Kacar, Y. A., Curuk, S., (2009), Regeneration and HistologicalAnalysis of Regeneration in Bottle Gourd (Lagenaria siceraria (Molina) Stand.). Turk J Agric For33(2009) pp. 165-172Moreno V & Roig LA (1990) Somaclonal variation in cucurbits; In: Biotechnology in agriculture andforestry Vol 11: Somaclonal variation in crop improvement I; pp 435?464 ed. Y P S Bajaj (Berlin,Heidelberg:Springer? Verlag).Murashige T, Skoog F. (1962) A revised medium for rapid growth and bioassays with tobacco tissuecultures. Physiol. Plant.; 15: 473-479.Murashige, T. (1974). Plant propagation through tissue cultures. Ann. Rev. Plant Physiol.25: 135-166.Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobaccocultures, Physiol. Plant. 15:473-497Murashige, T.; Skoog, F. (1962), A revised medium for rapid growth and bioassays with tobaccotissue cultures. Physiol. Plant, 15, 473-497.Nakamura, K., Wakita, Y. & Yokota, S. (1995). Induction of multiple shoots by shoot apex culture inMagnolia obovata Thunb. Plant Tissue Culture Letters. 12: 34- 40.Nishibayashi, S., H. Kaneko & T. Hayakawa. (1996). Transformation of cucumber (Cucumissativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. PlantCell Rep. 15: 809-814.Nitsch JP 1951. Growth and development in vitro of excised ovaries. Am. J. Bot. 38: 566?577.Nitsch, J.P. & Nitsch, C (1969) Haploid plants from pollen grains. Science. 163: 8587Nobcourt, P. (1939). Sur la prennit et laugmentation de volume des cultures de tissuesvgtaux. Comptes Rendus des Sances de la Socit de Biologie et de ses Filiales, 130, 12701271.Okere, A. U. & Adegeye, A. (2011). In vitro propagation of an endangered medicinal timber speciesKhaya grandifoliola C. Dc. African Journal of Biotechnology Vol. 10(17), pp. 3335-3339Philips, Greogory, C. (2004). In vitro morphogenesis in plants recent advances. In Vitro Cellularand Development Biology Plant. 40: 342-345Pierik, R. I. M. (1987). In vitro Culture of Higher Plants. Martinus Nijhoff Publishers,Dordrecht, ppt:1-344Puhan, Z., & Martin. S. M. (1971). The industrial potential of plant cell culture. Prog.Ind. Microbiol. 9: 13-39.Reddy M. C., Murthy K. S. R & Pullalah T (2012), Synthetic seeds : A review in agriculture andforestry, African Journal of Biotechnology Vol. 11(78), pp. 14254-14275S. Habibur Rahman (2003), Bottle Gourd (Lagenaria siceraria) A vegetable good for health, Researchand Development, Amal Herbal Formulation Pvt. Ltd., M. G. Road, Ghatkopar, MumbaiSaidin, I. Sayuran tradisional, ulam dan penyedap rasa (2000). Penerbit Universiti kebangsaanMalaya. pp 1-33Sarowar S, Oh HY, Hyung NI, Min BW, Harn CH,Yang SK, Ok SH & Shin JS (2003) In vitromicropropagation of a Cucurbita interspecific hybrid cultivar ? a root stock plant. PlantCell Tiss. Organ Cult. 75: 179?182.Sarowar, S., H.Y. Oh, N.I. Hyung, B.W. Min, C.H. Harn, S.K. Yang, S.H.Ok & J.S. Shin. (2003). Invitro propagation of a Cucurbita interspecific hybrid cultivar-a root stock plant. Plant Cell,Tissue Org Cult. 75: 179-182.Schenk, R. V., & A. C. Hildebraudt. 1972. Medium and techniques for induction and Skoog, F., &Miller, C.O.(1957). Chemical regulation of growth and organ formationin plant tissue cultured in vitro . Symp. Exp Biol. 11:118-131.Skoog, F., Miller, C.O (1957). Chemical regulation of growth and organ formation in plant tissuecultured in-vitro. Symp. Exp Biol. 11:118-131Steiner AA, Winden H. (1970) Recipe for ferric salts of ethelenediaminetetracetic acid. PlantPhysiol.; 46: 862- 863.Steward, F.C., Mapes, M.O., & Mears, K. (1958). Growth and organized development of cultured cells.II. Organization in cultures grown from freely suspended cells, American Journal of Botany.45:705-708Street, H.E. (1973). Plant tissue and cell culture. Botanical Monogr. BlackwellScientific Publications, London. 2.Taji, A., Prakash, P.K. & Prakash, L. (2002). In Vitro Plant Breeding. Howarth Press Inc. New YorkTempe, J., Ed. (1972). Protoplastes et Fusion de Cellules Somatiques Vegetales.Colloq. Int. Cent. Natl. Rech. Sci., Paris. 222.Thomas, D.T & Philip B. (2005). Thidiazuron-induced high frequency shootorganogenesis from leaf-derived callus of a medicinal climber,Tylophora indica(Burm.F) Merrill.In Vitro Cell .Dev.Biol- Plant. 41:124-128.Thomas, T.D. & Jacob, A., 2004. Direct somatic embryogenesis of Curculigo orchioidesGaertn. an Endangered medicinal herb. J. Plant Biotech., 6 :193-197.Thorpe, T. A. (1990). The current status of plant tissue culture. In S. S. Bhojwani(Ed.), Plant tissue culture: Applications and limitations (pp. 133). Amsterdam: Elsevier.Torres KC. (1989), editor. Tissue culture techniques for horticultural crops. New York, London:Chapman and Hall.Torres, K.C. (1989). Tissue culture techniques for horticultural crops. AVI Books, New.York.Vasil, I. K., & V. Vasil. (1972) Totipotency and embryogenesis in plant cell and tissue cultures.In Vitro. 8: 117-125.Vinterhalter D, Vinterhalter BS. Micropropagation of Dracaena sp. (1997)In: Bajaj YPS (ed.)Biotechnology in agriculture and forestry 40, High-tech. and Micropropagation VI. Berlin,Heidelberg: Springer; . p131- 146.Wehner TC, Cade RM & Locy RD (1990) Cell, tissue and organ culture techniques for geneticWhite, P. R. 1963. The Cultivation of Animal and Plant Cells. 2nd ed. Ronald Press, New York.White, P.R. (1943). A Handbook of Plant Tissue Culture. Jacques Cattell Press,Lancaster, PA. pp:227 |