Plant tissue culture of pomegranate, Punica granatum L.

The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Comple...

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Main Author:	Muhammad Al-Amin Mazlan
Format:	thesis
Language:	eng
Published:	2018
Subjects:	SB Plant culture
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=5788
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id	oai:ir.upsi.edu.my:5788
record_format	uketd_dc
institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	SB Plant culture
spellingShingle	SB Plant culture Muhammad Al-Amin Mazlan Plant tissue culture of pomegranate, Punica granatum L.
description	The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Completely Randomized Design (CRD). The complete regeneration of the Punicagranatum L. plant and callus production was successfully produced through the tissue culturetechnique. Explants used in this study were leaves, stem and roots from 8-week-old asepticseedlings. All cultures were stored at a temperature of 25 1C and light 16 hours of light,8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat andvermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found tobe very responsive and Murashige and Skoog (MS) medium added with 2.0 mg/lBenzylaminoupurine (BAP) + 1.5 mg/l Napthalene Acetic Acid (NAA) has been identified asthe optimum medium for shoot regeneration. Leaf explant managed to produce the number ofshoots in vitro with 1.33330.3228 per explant at week 8. Whereas from stem explant, MSmedia added with 0.5 mg/l BAP and 2.0 mg/l NAA gave the highest shoot development with 1.0000 0.2837 shoots per explant. Combination of NAA and BAP, at 1.5 mg/l respectively wasused for complete regeneration of Punica granatum L. in vitro. In addition, artificialseeds of Punica granatum were also produced. As a conclusion, this method was proven to besuccessful in producing new generation of Punica granatum L. and its features are preserved. Thisproves that plant tissue culture technology could be an alternative solution to achievehigh quality of Punica granatum L, therefore could increase the crop production.
format	thesis
qualification_name
qualification_level	Master's degree
author	Muhammad Al-Amin Mazlan
author_facet	Muhammad Al-Amin Mazlan
author_sort	Muhammad Al-Amin Mazlan
title	Plant tissue culture of pomegranate, Punica granatum L.
title_short	Plant tissue culture of pomegranate, Punica granatum L.
title_full	Plant tissue culture of pomegranate, Punica granatum L.
title_fullStr	Plant tissue culture of pomegranate, Punica granatum L.
title_full_unstemmed	Plant tissue culture of pomegranate, Punica granatum L.
title_sort	plant tissue culture of pomegranate, punica granatum l.
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Teknikal dan Vokasional
publishDate	2018
url	https://ir.upsi.edu.my/detailsg.php?det=5788
_version_	1747833228126846976
spelling	oai:ir.upsi.edu.my:57882021-04-08 Plant tissue culture of pomegranate, Punica granatum L. 2018 Muhammad Al-Amin Mazlan SB Plant culture The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Completely Randomized Design (CRD). The complete regeneration of the Punicagranatum L. plant and callus production was successfully produced through the tissue culturetechnique. Explants used in this study were leaves, stem and roots from 8-week-old asepticseedlings. All cultures were stored at a temperature of 25 1C and light 16 hours of light,8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat andvermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found tobe very responsive and Murashige and Skoog (MS) medium added with 2.0 mg/lBenzylaminoupurine (BAP) + 1.5 mg/l Napthalene Acetic Acid (NAA) has been identified asthe optimum medium for shoot regeneration. Leaf explant managed to produce the number ofshoots in vitro with 1.33330.3228 per explant at week 8. Whereas from stem explant, MSmedia added with 0.5 mg/l BAP and 2.0 mg/l NAA gave the highest shoot development with 1.0000 0.2837 shoots per explant. Combination of NAA and BAP, at 1.5 mg/l respectively wasused for complete regeneration of Punica granatum L. in vitro. In addition, artificialseeds of Punica granatum were also produced. As a conclusion, this method was proven to besuccessful in producing new generation of Punica granatum L. and its features are preserved. Thisproves that plant tissue culture technology could be an alternative solution to achievehigh quality of Punica granatum L, therefore could increase the crop production. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=5788 https://ir.upsi.edu.my/detailsg.php?det=5788 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional Abd El-Gawad, N.A., N.S. Zaied and M.A. Saleh, 2010. A comparative study on differentcarbon source concentrations and gelling agent on in vitro proliferation of pineapple (Ananascomosus). The Journal of Nat we and Science, 8(2): 1-5Acheapong E. (1982). Multiplication and Conservation of Cocoyam (Xarthosomasajiltifoljum) germplasm by application of tissue culture methods Ph.D Thesis. Universityof Burmingham, UKAkoroda M. O. (1990). Ethno botany of Telfaria Occidtendalic Among Igbos of Nigeria.Econ. Bot. 44 (1): 29-39.Aladele S. E. and Kuta D. (2008). Environmental and genotypic effects on the rowth rate of in vitrocassava plantlet (Manihot esceulenta) African Journal of Biotechnology Vol. 7 (4), pp. 381-385.Al-Khateeb, A.A., (2008). Regulation of in vitro bud formation of date palm (Phoenixdactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14): 6550-6555.Al-Khateeb, A.A., (2008). Regulation of in vitro bud formation of date palm (Phoenixdactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14): 6550-6555.Al-Khayri, (2003). In vitro germination of somatic embryos in date palm : Effect of auxinconcentration and strength of MS salts. Current Science, 84(3): 680-683.Amin MN, Islam MN, Azad MAK (1999). Regeneration of plantlets in vitro from theseedling explants of pomegranate (Punica granatum L.). Plant Tissue Cult. 9(1): 53-61.Andreu, P., J.A. Marn (2005). In vitro culture establishment and multiplication of thePrunus rootstock Adesoto 101 (P. insititia L.) as affected by the type of propagationof the donor plant and by the culture medium composition. Scientia Horticulturae, 106(2): 258-267.Ara H, Jaiswal Uma, Jaiswal VS ( 2000 ) Synthetic seed: Prospects and limitations, Current Science78, 1438 1444Attree, S.M.; Pomeroy, M.K.; Fowke, L.C. ( 1994 ) Production of vigoous, dessication tolerant whitespruce (Picea-glauca (moench) voss) synthetic seeds in a bioreactor.Plant Cell Rep., 13, 601606.Baaya, M.H.M. (2002). Studies on propagation of some fruit species by using tissue culturetechniques. M. Sci. Thesis, Hort. Dept., Fac. of Agric., Moshtohor, Zagazig University.Babak V.K, Ahmad E. And Masoud T. ( 2013 ) In Vitro Propagation of Pomegranate ( Punica granatum) Cv. Males Yazdi. Deparments of Horticulture Science, Faculty of Agriculture, Universityof Bu-Ali Sina, Hamedan, Iran.Babbar, S.B. and S.C. Gupta (1986) . Effect of Carbon Source on Datura metel MicrosporeEmbryogenesis and the Growth of Callus Raised from Microspore-derived Embryos.Biochemie und Physiologie der Pflanzen, 181(5): 331-338.Bhatia P, Ashwath N (2005). Effect of medium pH on shoot regeneration from thecotyledonary explants of Tomato. Biotechnol., 4: 7-10.Bridgen, Mark P. (1994). "A Review of Plant Embryo Culture". Hortscience 29: 1243 1246.Brown DCW, Leung DWM, Thorpe TA (1979). Osmotic requirement for shoot formation in tobacco callus.Physiol. Planta, 46: 36-41.Chang, P.Y, Fujita, S., Kohno, K., Ashrafuzzaman, M.D., Nakamura, N. And Hayashi, N. (2000)Purification and characterization of polyphenol oxidase from banana ( Musa sapientum L.)pulp.Journal of Agricultural and Food Chemistry 48: 2732-2735.Christian Raquin (1983). Utilization of Different Sugars as Carbon Source for in vitroAnther Culture of Petunia. Zeitschrift fr Pflanzenphysiologie, 111(5): 453-457.Conger, B. V. (1980). Cloning Agricultural Plants via In-vitro Techniques, CRC Press Inc, BocaRaton, Florida, pp; 1-50Dalmacio I. F. (1992). Biotechnology for sustainable Agriculture National Institute ofBiotechnology and Applied Microbiology, University of the Philippines.Dalziel, J.M. 1937. The useful plants of West Tropical Africa. Whitefriars Press, London, 612 pp.Dalziel J.M. (1937) The useful plants of West Tropical Africa. Whitefriars press,London.612pp.Fagbemi T.N.F., Eleyinmi A.F., Atum H. N., Akpambang O. (2005) Nutritionalcomposition of fermented fluted pumpkin (Telfairiaoccidentalis) seeds forproductionof ogiriugu. IFT Annual Meeting, July 15-20 New Orleans,Louisana.Frusciante, L., Barone, A.,Carputo,D. Ercolano, M.R., Della Rocca, F., & Esposito, S.(2010). Evaluation and use of Plant Biodiversity for Food and Pharmaceutical. Fitoterpia.71 : 66 S72.Fuentes, S.R.L., M.B.P. Calheiros, J. Manetti-Filho and L.G.E. Vieira (2000). The effect of silvernitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora. Plant CellTiss. Org. Cult., 60: 5-13.Gamborg, O.L., T. Murshige, T.A. Thorpe and I.K. Vasil (1976). Plant tissue culturemedia. In vitro, 12(7): 473- 478.Gautheret, R. J. (1934). Culture du tissus cambial. Comptes Rendus Hebdomadaires des Sances del'Acadmie des Sciences, 198, 21952196.Gautheret, R. J. (1935). Recherches sur la culture des tissus vgtaux. Ph.D. Thesis, ParisGautheret, R. J. (1939). Sur la possibilit de raliser la culture indfinie destissus de tubercules de carotte. Comptes Rendus Hebdomadaires des Sances de l'Acadmie desSciences, 208, 118120.Gbile Z.O.(1998). Collection, Conservation and Utilization of medicinal plants. In Robert PA, &Janice EA (eds.). Conservation of Plant Genes III: Conservation and Utilization of AfricanPlants. Missouri Botanical Gardens Press. pp. 163-174.Gibson IS (2000). Plant sugar-response pathways. Part of a complex regulatory web. Plant Physiol.,124: 1532-1539.Gurel S, Gulsen Y (1998). The effects of different sucrose, agar and pH levels on in vitro shootproduction of Almond (Amygdalus communis L.). Tr. J. Bot., 22: 363-373.Haberlandt, G. (1902) Kulturversuche mit isolierten Pflanzenzellen. Sitzungsber. Akad.Wiss. Wien. Math.-Naturwiss. Kl., Abt. J. 111, 6992.Hassan, S., All. M. (2012). Studies on improving flowering and fruiting of lecont e pears.Ph.D. Hort., Dept., Fac. of Cairo Univ.IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, Switzerland.IUCN (2006). IUCN Red List of Threatened Species. IUCN, Gland, Switzerland.Jain, N., M.W. Bairu, W.A. Stirk, J. Van Staden (2009). The effect of medium, carbon source and explant on regeneration and control of shoot-tip necrosis in Harpagophytumprocumbens. South African Journal of Botany, 75(1): 117-121.M., K. Imizu and T. Hirano (2001). Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. ScientiaHorticulturae, 89(3,4): 207-215.Kumar HGA Murthy HN and Paek KY (2003 ). Embryogenesis and plant regenerationfrom anther cultures of Cucumis sativus L. Scientia Hort , 98: 213-222Levin, R., Stav, R., Alper, Y. and Watad, A. A. (1998). A technique for repeated nonaxenicsubculture of plant tissues in a bioreactor on liquid medium containing sucrose. Plant TissueCulture Biotechnology. (3):4145.Lloyd, G. and B.H. McCown 1(981). Commerciallyfeasiblemicropropagation of Mountain Laurel, Kalmialatifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc., 30: 421- 427.Lyam P.T.,Musa M. L., Jamaleddine, Z.O., Okere, A.U., Carlos A. and Odofin, W.T.(2012). The potentials of Temporary immersion bioreactor TIBS in meeting crop production demand inNigeria. Journal of biology and life science ISSN 2157 -6076 Vol. 3:1.Mantel A.H. and Smith H. Culture factors that influence secondary metabo lismaccumulation in plat cell and tissue culture. In: Mantell. A., Smith H(Eds). Plant biotechnology,Cambridge University Press, Cambridge, 1983; 75-110.Mantel S.H., Mathews J.A. and McKee R.A. (1985). Principles of plantBiotechnology,Blackwell Scientific Publications, London.Miyajuma, D. (2006). "Ovules that failed to form seeds in zinnia (Zinnia violacec Cav)".Sci Hortic 107 (2): 176182. doi:10.1016/j.scienta.2005.06.014.Mukherjee, P., N. Husain, S.C. Misra and V.S. Rao (2010). In vitro propagation of a graperootstock, deGrasset (Vitis champinii Planch.): Effects of medium compositions and plant growthregulators. Scientia Horticulturae, 126(1): 13-19.Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobaccotissue cultures. Physiol. Plant, 15: 473-497.Murashige, T. (1973). Nutrition of plant cells and organs in vitro. In Vitro, 9: 81-85.Murkute, A., Patil, S., Patil, B.N., Mayakumari, M.S., (2002) Micropropagation in pomegranate,callus induction and differentiation. South Indian Hort. 50:4955.Musa M. L., Lyam P.T., Jamaleddine, Z.O., Okere, A.U., Carlos A. and Odofin, W.T.(2012). The potentials of Temporary immersion bioreactor TIBS in meeting crop production demand inNigeria. Journal of biology and life science ISSN 2157 -6076 Vol. 3:1.Naik, S., Pattnaik, S., Chand, P., (2000) High frequency auxiliary shoot proliferation and plantregeneration from cotyledonary nodes of pomegranate. Sci. Hort. 79:175- 83.Nehanjali P., Kamlesh K. Ajay Kumar T. (2012 ) In Vitro Organogenesis from CotyledonDerived Callus Cultures of Punica granatum L. cv. Kandhari Kabuli. National Academy ofScience, India.Nehanjali P., Kamlesh K. Ajay Kumar T. (2013 ) Direct Organogenesis in Punica granatumL. cv. Kandhari Kabuli from Hypocotyl Explants. National Academy of Science, IndiaNowak B, Milczzynski K, Hudy L (2004). Sugar uptake and utilization during adventitious buddifferentiation on in vitro leaf explants of Wegierka Zwykla plum (Prunus domestica).Plant Cell, Tissue Organ Cult., 76: 255-260.Okere, A. U. and Adegeye, A. (2011). In vitro propagation of an endangered medicinal timber speciesKhaya grandifoliola C. Dc. African Journal of Biotechnology Vol. 10(17), pp. 3335-3339.Omura, M., Matsuta, N., Moriguchi, T., Kuzaki, I., Sanada, T., (1987) Establishment of tissueculture methods in dwarf pomegranate (Punica granatum L. var. ?Nana?) and application for theinduction of variants. Bull. Fruit Tree Res. 14:17-44.Pajimi O. and Fashola T. R. (2010). In vitro micro-propagation of Plukenetia conophora Mull.Arg.Journal of South Pacific Agriculture. 14(1&2):1-5Petersen, K.K., J. Hansen, A. Bouvet, R. Galvayrac, M. Paques (2001). Significance of differentcarbon sources and sterilization methods on callus induction and plant regeneration of MiscanthusX ogiformis Honda Giganteus. Plant Cell Tiss. Org. Cult., 58: 189-197.Redenbaugh, K.; Paasch, B.D.; Nichol, J.W.; Kossler, M.E.; Viss, P.R.;Walker, K.A. (1986 ) Somatic seeds-encapsulation of asexual plant embryos. Nat. Biotechnol, 4, 797801.[CrossRef]Redenbaugh, K.; Fujii, J.A.; Slade, D ( 1988 ). Encapsulated plant embryos. In Advances inBiotechnological Processes; Mizrahi, A., Ed.; Alan R. Liss Inc.: New York, NY, USA; pp. 225248.Redenbaugh, K.; Fujii, J.A.; Slade, D.( 1993 ) Hydrated coating for synthetic seeds. In Synseeds:Application of the Synthetic Seeds to Crop Improvement; CRC Press: Boca Raton, FL, USA, ; pp.305327.Raj D. And Kamlesh K. ( 2010 ). In Vitro Regeneration of Punica granatum plants from different Juvenile Explants. Department of Biotechnology, University of Horticulture andForestry, India.Reed, Sandra (2005). Robert N. Trigiano and Dennis J. Gray, ed. Plant Development andBiotechnology. CRC Press. pp. 235239. ISBN 0-8493-1614-6.Reinert, J. and P.R. White (1956). The cultivation in vitro of tumor tissues and normaltissues of Picea glauca. Physiol. Plant., 9: 117-189.Reynolds T.L. Am. J. Bot. 1982; 73: 914-918Risser, P.G. and P.R. White (1964). Nutritional requirements of spruce tumor cellsin vitro.Physiol. Plant. 17: 620-635.Sakano K (1990). Proton/phosphate stoichiometry in uptake of inorganic phosphate bycultured cells of Catharanthus roseus (L.) G. Don. Plant Physiol., 93: 479-483.Shah MI, Jabeen M, Ilahi I. 2003. In v?tro callus induction, its proliferation andregeneration in seed explants of wheat (Triticum aestivum L.) variety Lu-26S. Plant Journal ofBotany 35(2), 209- 217.Sanusi I. S., Odofin W. T,, Aladele S. E, , Olayode M. N., Gamra E. O., Fajimi O. (2008) In vitroculture of Telferia occidentalis under different cytokinins and auxin combination. Africanjournal of biotechnology. 7 (14):2407-2408Singh N.V., Singh S.K., Patel V.B. 2007. In vitro axillary shoot proliferation and clonalpropagation of G-137 pomegranate (Punica granatum). INDIANJ. AGRIC. SCI. 77(8): 505-508.Stavarek SJ, Croughan TP, Rains DW (1980). Regeneration of plants from long-termcultures of alfalfa cells. Plant Sci. Lett., 19: 253-261.Steward, F.C., Mapes, M.O., and Mears, K. (1958). Growth and organized development of culturedcells. II. Organization in cultures grown from freely suspended cells, American Journal ofBotany. 45:705-708Tange, H., Y. Low and C. Lui (2008). Plant regenerate from in vitro leaves of fourcommercial pyrus species. Plant Soil Environs, 54(4): 140-148.Teisson, C. and Alvard, D. (1995). A new concept of plant in vitro cultivation in liquid medium:Temporary Immersion. In: Current Issues in Plant Molecular and Cellular Biology.Terzi, M., Cella,R. and Falavigna, A. (Editors). Kluwer Academic Publisher ppThomas, D.T & Philip B. (2005). Thidiazuron-induced high frequency shoot organogenesis from leaf-derived callus of a medicinal climber,Tylophora indica (Burm.F) Merrill.In VitroCell .Dev.Biol- Plant. 41:124-128.Vespasiano, B.d.N. and W.C. Otoni (2003). Carbon sources and their osmotic potential inplant tissue culture: does it matter?. Scientia Horticulturae, 97(3-4): 193-202.White, P.R. (1963). The Cultivation of Animal and Plant Cells. 2nd ed. Ronald Press,New York.Yang ZH, Ludders P (1993).Organogenesis of Punica granatum L. var.Nana.Angewandte Botanik, 67(5-6): 151-156.Zhu LW, Zhang SM, Song FS, Gong XM, Fang WJ, Sun J, Li SW (2003).Regeneration system of pomegranate by in vitro culture. Acta. Hort. Sinica,430(2): 207-208.

Plant tissue culture of pomegranate, Punica granatum L.

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