Plant tissue culture of pomegranate, Punica granatum L.

The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Comple...

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Main Author: Muhammad Al-Amin Mazlan
Format: thesis
Language:eng
Published: 2018
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Online Access:https://ir.upsi.edu.my/detailsg.php?det=5788
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institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language eng
topic SB Plant culture
spellingShingle SB Plant culture
Muhammad Al-Amin Mazlan
Plant tissue culture of pomegranate, Punica granatum L.
description The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Completely Randomized Design (CRD). The complete regeneration of the Punicagranatum L. plant and callus production was successfully produced through the tissue culturetechnique. Explants used in this study were leaves, stem and roots from 8-week-old asepticseedlings. All cultures were stored at a temperature of 25 1C and light 16 hours of light,8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat andvermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found tobe very responsive and Murashige and Skoog (MS) medium added with 2.0 mg/lBenzylaminoupurine (BAP) + 1.5 mg/l Napthalene Acetic Acid (NAA) has been identified asthe optimum medium for shoot regeneration. Leaf explant managed to produce the number ofshoots in vitro with 1.33330.3228 per explant at week 8. Whereas from stem explant, MSmedia added with 0.5 mg/l BAP and 2.0 mg/l NAA gave the highest shoot development with 1.0000 0.2837 shoots per explant. Combination of NAA and BAP, at 1.5 mg/l respectively wasused for complete regeneration of Punica granatum L. in vitro. In addition, artificialseeds of Punica granatum were also produced. As a conclusion, this method was proven to besuccessful in producing new generation of Punica granatum L. and its features are preserved. Thisproves that plant tissue culture technology could be an alternative solution to achievehigh quality of Punica granatum L, therefore could increase the crop production.
format thesis
qualification_name
qualification_level Master's degree
author Muhammad Al-Amin Mazlan
author_facet Muhammad Al-Amin Mazlan
author_sort Muhammad Al-Amin Mazlan
title Plant tissue culture of pomegranate, Punica granatum L.
title_short Plant tissue culture of pomegranate, Punica granatum L.
title_full Plant tissue culture of pomegranate, Punica granatum L.
title_fullStr Plant tissue culture of pomegranate, Punica granatum L.
title_full_unstemmed Plant tissue culture of pomegranate, Punica granatum L.
title_sort plant tissue culture of pomegranate, punica granatum l.
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Teknikal dan Vokasional
publishDate 2018
url https://ir.upsi.edu.my/detailsg.php?det=5788
_version_ 1747833228126846976
spelling oai:ir.upsi.edu.my:57882021-04-08 Plant tissue culture of pomegranate, Punica granatum L. 2018 Muhammad Al-Amin Mazlan SB Plant culture The purpose of this study was to examine the propagation ability of Punica granatumL. through tissue culture system. In addition, callus induction was also carried out in the studyto observe the rate of cell differentiation on explants. The experimental design that was used inthis research is Completely Randomized Design (CRD). The complete regeneration of the Punicagranatum L. plant and callus production was successfully produced through the tissue culturetechnique. Explants used in this study were leaves, stem and roots from 8-week-old asepticseedlings. All cultures were stored at a temperature of 25 1C and light 16 hours of light,8 hours dark. Plant acclimatization was done on three different medium, garden soil, coco peat andvermiculite. All data was recorded and analyzed using ANOVA. Leaf explants were found tobe very responsive and Murashige and Skoog (MS) medium added with 2.0 mg/lBenzylaminoupurine (BAP) + 1.5 mg/l Napthalene Acetic Acid (NAA) has been identified asthe optimum medium for shoot regeneration. Leaf explant managed to produce the number ofshoots in vitro with 1.33330.3228 per explant at week 8. Whereas from stem explant, MSmedia added with 0.5 mg/l BAP and 2.0 mg/l NAA gave the highest shoot development with 1.0000 0.2837 shoots per explant. Combination of NAA and BAP, at 1.5 mg/l respectively wasused for complete regeneration of Punica granatum L. in vitro. In addition, artificialseeds of Punica granatum were also produced. As a conclusion, this method was proven to besuccessful in producing new generation of Punica granatum L. and its features are preserved. Thisproves that plant tissue culture technology could be an alternative solution to achievehigh quality of Punica granatum L, therefore could increase the crop production. 2018 thesis https://ir.upsi.edu.my/detailsg.php?det=5788 https://ir.upsi.edu.my/detailsg.php?det=5788 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional Abd El-Gawad, N.A., N.S. Zaied and M.A. Saleh, 2010. A comparative study on differentcarbon source concentrations and gelling agent on in vitro proliferation of pineapple (Ananascomosus). The Journal of Nat we and Science, 8(2): 1-5Acheapong E. (1982). Multiplication and Conservation of Cocoyam (Xarthosomasajiltifoljum) germplasm by application of tissue culture methods Ph.D Thesis. Universityof Burmingham, UKAkoroda M. O. (1990). Ethno botany of Telfaria Occidtendalic Among Igbos of Nigeria.Econ. Bot. 44 (1): 29-39.Aladele S. E. and Kuta D. (2008). 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