Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
This study performed to investigate the chemical compounds, toxicology, in vitro andin vivo antioxidant capacity of Malaysian Peperomia pellucida plant towards ICR mice.The qualitative chemical compound test was performed and the antioxidant capacity of P.pellucida plant extract was analyzed by 2,2-...
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| Format: | thesis |
| Language: | eng |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=6146 |
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| Summary: | This study performed to investigate the chemical compounds, toxicology, in vitro andin vivo antioxidant capacity of Malaysian Peperomia pellucida plant towards ICR mice.The qualitative chemical compound test was performed and the antioxidant capacity of P.pellucida plant extract was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), -carotenebleaching and oxygen radical absorbance capacity (ORAC) assays. The in vivo sub-acutetoxicity study that used the best solvent from in vitro assay was done using ICR mice which wererandomly divided. The different doses of 50 mg/kg, 150 mg/kg, 200 mg/kg, 500 mg/kg and 1000 mg/kgbody weight of extract were administered daily for 21 days to the treated mice. The antioxidantenzymes in liver and kidney of mice were investigated using standard assays. P. pellucida onlyexhibited the presence of alkaloid, phenol and saponin. The ability to scavenge free radical inDPPH assay showed that methanol extract of Selangor (ground) sample depicted the highest value of1108 5.568 mg/ml compared to the other samples. In -carotene bleaching assay, the ethylacetate extract of Penang (ground) sample offered the highest percentage (86.111%) ofscavenging activity from free radical compared to the other samples. In ORAC assay,methanol extract of Penang (ground) sample showed the highest value 397.691 mol of Troloxequivalent/l. Findings showed that ethyl acetate and methanol extracts exhibited a good solventfor antioxidant capacity of P. pellucida. However, in sub-acute toxicity study, ethylacetate and methanol extract of higher doses lead to mortality in mice. Furthermore, theadministration of ethyl acetate and methanol extract of P. pellucida did not affect the antioxidantenzymes in liver and kidney of mice. In conclusion, the antioxidant capacity of P. pellucida canonly be seen by in vitro assay. Thus, P. pellucida could be a potential source ofantioxidant substance. |
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