Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth

This study performed to investigate the chemical compounds, toxicology, in vitro andin vivo antioxidant capacity of Malaysian Peperomia pellucida plant towards ICR mice.The qualitative chemical compound test was performed and the antioxidant capacity of P.pellucida plant extract was analyzed by 2,2-...

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Main Author:	Amirah Shaari
Format:	thesis
Language:	eng
Published:	2019
Subjects:	QK Botany
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=6146
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institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	QK Botany
spellingShingle	QK Botany Amirah Shaari Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
description	This study performed to investigate the chemical compounds, toxicology, in vitro andin vivo antioxidant capacity of Malaysian Peperomia pellucida plant towards ICR mice.The qualitative chemical compound test was performed and the antioxidant capacity of P.pellucida plant extract was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), -carotenebleaching and oxygen radical absorbance capacity (ORAC) assays. The in vivo sub-acutetoxicity study that used the best solvent from in vitro assay was done using ICR mice which wererandomly divided. The different doses of 50 mg/kg, 150 mg/kg, 200 mg/kg, 500 mg/kg and 1000 mg/kgbody weight of extract were administered daily for 21 days to the treated mice. The antioxidantenzymes in liver and kidney of mice were investigated using standard assays. P. pellucida onlyexhibited the presence of alkaloid, phenol and saponin. The ability to scavenge free radical inDPPH assay showed that methanol extract of Selangor (ground) sample depicted the highest value of1108 5.568 mg/ml compared to the other samples. In -carotene bleaching assay, the ethylacetate extract of Penang (ground) sample offered the highest percentage (86.111%) ofscavenging activity from free radical compared to the other samples. In ORAC assay,methanol extract of Penang (ground) sample showed the highest value 397.691 mol of Troloxequivalent/l. Findings showed that ethyl acetate and methanol extracts exhibited a good solventfor antioxidant capacity of P. pellucida. However, in sub-acute toxicity study, ethylacetate and methanol extract of higher doses lead to mortality in mice. Furthermore, theadministration of ethyl acetate and methanol extract of P. pellucida did not affect the antioxidantenzymes in liver and kidney of mice. In conclusion, the antioxidant capacity of P. pellucida canonly be seen by in vitro assay. Thus, P. pellucida could be a potential source ofantioxidant substance.
format	thesis
qualification_name
qualification_level	Master's degree
author	Amirah Shaari
author_facet	Amirah Shaari
author_sort	Amirah Shaari
title	Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
title_short	Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
title_full	Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
title_fullStr	Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
title_full_unstemmed	Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth
title_sort	phytochemical screening,antioxidant capacity and toxicological study of malaysian peperomia pellucida (l.) kunth
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Sains dan Matematik
publishDate	2019
url	https://ir.upsi.edu.my/detailsg.php?det=6146
_version_	1747833246237851648
spelling	oai:ir.upsi.edu.my:61462021-07-30 Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth 2019 Amirah Shaari QK Botany This study performed to investigate the chemical compounds, toxicology, in vitro andin vivo antioxidant capacity of Malaysian Peperomia pellucida plant towards ICR mice.The qualitative chemical compound test was performed and the antioxidant capacity of P.pellucida plant extract was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), -carotenebleaching and oxygen radical absorbance capacity (ORAC) assays. The in vivo sub-acutetoxicity study that used the best solvent from in vitro assay was done using ICR mice which wererandomly divided. The different doses of 50 mg/kg, 150 mg/kg, 200 mg/kg, 500 mg/kg and 1000 mg/kgbody weight of extract were administered daily for 21 days to the treated mice. The antioxidantenzymes in liver and kidney of mice were investigated using standard assays. P. pellucida onlyexhibited the presence of alkaloid, phenol and saponin. The ability to scavenge free radical inDPPH assay showed that methanol extract of Selangor (ground) sample depicted the highest value of1108 5.568 mg/ml compared to the other samples. In -carotene bleaching assay, the ethylacetate extract of Penang (ground) sample offered the highest percentage (86.111%) ofscavenging activity from free radical compared to the other samples. In ORAC assay,methanol extract of Penang (ground) sample showed the highest value 397.691 mol of Troloxequivalent/l. Findings showed that ethyl acetate and methanol extracts exhibited a good solventfor antioxidant capacity of P. pellucida. However, in sub-acute toxicity study, ethylacetate and methanol extract of higher doses lead to mortality in mice. Furthermore, theadministration of ethyl acetate and methanol extract of P. pellucida did not affect the antioxidantenzymes in liver and kidney of mice. In conclusion, the antioxidant capacity of P. pellucida canonly be seen by in vitro assay. Thus, P. pellucida could be a potential source ofantioxidant substance. 2019 thesis https://ir.upsi.edu.my/detailsg.php?det=6146 https://ir.upsi.edu.my/detailsg.php?det=6146 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik A'attiyyah, A. A., Wan Afiqah Syahirah WG., Nor Azah M. A., Kannan T. P., Suharni,M., & Ahmad, A. (2017). Phytochemical properties and traditional uses of selected medicinalplants in Malaysia: A review. Journal of Biomedical and Clinical Sciences, 2(2), 14-25.Abas Hussin. (2001). Adverse effects of herbs and drug-herbal interactions. Malaysian Journal of Pharmacy, 1(2), 39-44.Abdille, Md. H., Singh, R. P., Jayaprakasha, G. K., & Jena, B. S. (2005). Antioxidant activity ofthe extracts from Dillenia indica fruits. Food chemistry, 90, 891-896.Abere, T. A., & Okpalaonyagu, S. O. (2015). 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Phytochemical screening,antioxidant capacity and toxicological study of Malaysian Peperomia pellucida (L.) Kunth

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