Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system

Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system

The research aimed to study the morphogenesis and growth optimization of Caricapapaya L. var Eksotika through tissue culture system. A study was conducted to testthe effect of 16 different concentrations and combinations of Benzylaminopurine(BAP) and Napthalene-Acetic Acid (NAA) on three different t...

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Main Author: Nurul Shifa Radzuan
Format: thesis
Language:eng
Published: 2019
Subjects:
SB Plant culture
Online Access:https://ir.upsi.edu.my/detailsg.php?det=6555
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institution Universiti Pendidikan Sultan Idris
collection UPSI Digital Repository
language eng
topic SB Plant culture
spellingShingle SB Plant culture
Nurul Shifa Radzuan
Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
description The research aimed to study the morphogenesis and growth optimization of Caricapapaya L. var Eksotika through tissue culture system. A study was conducted to testthe effect of 16 different concentrations and combinations of Benzylaminopurine(BAP) and Napthalene-Acetic Acid (NAA) on three different types of Carica papayaL. var Eksotika explants, namely leaf, stem and root. Complete plant regeneration wassuccessfully achieved when root, stem and leaf aseptic explants were cultured on MSmedium supplemented with various combinations of BAP and NAA. MS mediumsupplemented with 2.0 mg/L BAP was found to be the optimum medium and stemexplants was the most responsive explants producing 3.800 0.323 shoots perexplants. MS media supplemented with 0.5 mg/L BAP and 2.0 mg/L NAA was mostoptimum for root induction. Optimum callus induction was obtained when rootexplants were culture on MS medium fortified with 2.0 mg/L BAP + 1.0 mg/L NAA.Meanwhile, synthetic seeds of Carica papaya L. were produced when microshootswere encapsulated with 4.0% sodium alginate solution added with 2.0 mg/L BAP.Finally, acclimatization of Carica papaya L. was accomplished when plantlets weretransferred to the soil. The research showed that in vitro propagation of Caricapapaya L. through tissue culture system was successfully achieved. In conclusion, theregeneration process, callus induction, synthetic seed, microscopic studies andacclimatization of Carica papaya L. var Eksotika were achieved. Micropropagationhad been successfully obtained from the explants culture on combination andconcentration of BAP and NAA. This research has provided positive implicationstowards the development of agriculture industry especially in Malaysia and alsoworldwide.
format thesis
qualification_name
qualification_level Master's degree
author Nurul Shifa Radzuan
author_facet Nurul Shifa Radzuan
author_sort Nurul Shifa Radzuan
title Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
title_short Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
title_full Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
title_fullStr Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
title_full_unstemmed Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system
title_sort morphogenesis studies and growth optimization of carica papaya l. var eksotika through tissue culture system
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Teknikal dan Vokasional
publishDate 2019
url https://ir.upsi.edu.my/detailsg.php?det=6555
_version_ 1747833278352588800
spelling oai:ir.upsi.edu.my:65552022-01-06 Morphogenesis studies and growth optimization of Carica papaya L. var Eksotika through tissue culture system 2019 Nurul Shifa Radzuan SB Plant culture The research aimed to study the morphogenesis and growth optimization of Caricapapaya L. var Eksotika through tissue culture system. A study was conducted to testthe effect of 16 different concentrations and combinations of Benzylaminopurine(BAP) and Napthalene-Acetic Acid (NAA) on three different types of Carica papayaL. var Eksotika explants, namely leaf, stem and root. Complete plant regeneration wassuccessfully achieved when root, stem and leaf aseptic explants were cultured on MSmedium supplemented with various combinations of BAP and NAA. MS mediumsupplemented with 2.0 mg/L BAP was found to be the optimum medium and stemexplants was the most responsive explants producing 3.800 0.323 shoots perexplants. MS media supplemented with 0.5 mg/L BAP and 2.0 mg/L NAA was mostoptimum for root induction. Optimum callus induction was obtained when rootexplants were culture on MS medium fortified with 2.0 mg/L BAP + 1.0 mg/L NAA.Meanwhile, synthetic seeds of Carica papaya L. were produced when microshootswere encapsulated with 4.0% sodium alginate solution added with 2.0 mg/L BAP.Finally, acclimatization of Carica papaya L. was accomplished when plantlets weretransferred to the soil. The research showed that in vitro propagation of Caricapapaya L. through tissue culture system was successfully achieved. In conclusion, theregeneration process, callus induction, synthetic seed, microscopic studies andacclimatization of Carica papaya L. var Eksotika were achieved. Micropropagationhad been successfully obtained from the explants culture on combination andconcentration of BAP and NAA. This research has provided positive implicationstowards the development of agriculture industry especially in Malaysia and alsoworldwide. 2019 thesis https://ir.upsi.edu.my/detailsg.php?det=6555 https://ir.upsi.edu.my/detailsg.php?det=6555 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional Ali, M., Boonerjee, S., Islam, M. N., Saha, M. L., Hoque, M. I., and Sarker, R. H.(2018). Endogenous Bacterial Contamination of Plant Tissue CultureMaterials:Identification and Control Strategy. Plant Tissue Culture andBiotechnology,28(1), 99. doi:10.3329/ptcb.v28i1.37202Anandan, R., Thirugnanakumar, S., Sudhakar, D., and Balasubramanian, P. (2013). Invitro organogenesis and plantlet regeneration of (Carica papaya L.). Journal ofAgricultural Technology, 7(5), 1339-1348.Anis, M., and Ahmad, N. (2016). Plant Tissue Culture: A Journey from Research toCommercialization. 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