Phytochemical study of Phyllanthus amarus and its anticancer activity
This study aimed to isolate and purify compounds from Phyllanthus amarus and theirpotential as anticancer agents. About 2.01 kg of P. amarus from Euphorbiaceae family was collected from Melor, Kelantan. The sample was dried at room temperature,grinded and extracted with hexane, ethyl acetate, ethano...
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| Format: | thesis |
| Language: | eng |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=6699 |
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| Summary: | This study aimed to isolate and purify compounds from Phyllanthus amarus and theirpotential as anticancer agents. About 2.01 kg of P. amarus from Euphorbiaceae family was collected from Melor, Kelantan. The sample was dried at room temperature,grinded and extracted with hexane, ethyl acetate, ethanol and methanol. Compounds were purifiedusing various chromatographic techniques. The structures of the compound were determined byspectroscopic techniques including nuclear magnetic resonance, ultraviolet, infrared, massspectrometry and comparison with previous study. In addition, compounds were tested by theiranticancer effect against HeLa cells and NIH/3T3 cells by MTT assay. The results showedthat isolation of hexane and ethanol extract have produced seven compounds which were tanacetene,triglyceride fatty acids, mixtures of stigmasterol and -sitosterol, hypophyllanthin,niranthin, lintetralin, and nirtetralin. The MTT assay results showed that hypophyllanthin has a strong anticancer effect on HeLa cells compared to niranthin andlintetralin. In conclusion, P. amarus consisted of sesquiterpene, glyceride, sterols and lignans.Meanwhile, hypophyllanthin showed a potential as anticancer agent. In implication, the compoundscan be used as future reference for secondary metabolites of Malaysian plants and their potential as anticancer agents to be applied inpharmaceutical field. |
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