Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
Ficus carica L. is one of Moraceae family which natively to Westerns Asia anddistributed worldwide and economically important especially in sericulture, horticulture, food andcosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins. Thisplant also produces secondary...
Saved in:
| Main Author: | |
|---|---|
| Format: | thesis |
| Language: | eng |
| Published: |
2020
|
| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=6875 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Ficus carica L. is one of Moraceae family which natively to Westerns Asia anddistributed worldwide and economically important especially in sericulture, horticulture, food andcosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins. Thisplant also produces secondary metabolites, latex cells. The richness of nutrients andlatex cells make this plant possessed many pharmacological effects which is importantly tomedicinal and drugs industries. The demand for this plant is very high despite theproduction of fig is very inconsistent from year to year. So, this study aims to establish anddetermine the optimum concentration of benzylaminopurine (BAP) and napthelene acetic acid(NAA) for Ficus carica L. through plant tissue culture system by following completely randomizeddesign. The shoots were able to be regenerated from stems explants at most optimum concentrationof 1.0 mg/L BAP. For callus induction, all stem, shoot, petiole, and root explants able to emergecalli when cultured in MS medium with optimum combinations of BAP and NAA; 1.0 mg/L BAP +0.5 mg/L NAA, 0.5 mg/L BAP + 1.0 mg/L NAA, 1.5 mg/L BAP + 1.0 mg/L NAA, and 1.0 mg/L BAP + 1.0mg/L NAA respectively. The explants also were tested with other plant growth regulators such as thidiazuron (TDZ), Kinetin, indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) for a comparison. The results still showed that BAP and NAA werethe best plant growth regulators used compared to others combinations. The micromorphology studyshows the presence of trichomes on adaxial and abaxial of in vitro and in vivo leaves. Stoma wasalso visible on all surfaces except on adaxial of in vivo leaf. The acclimatization process allowedthe plantlets survived in garden soil with 86.67% after weeks 8 cultured. Lastly, synthetic seedsof Ficus carica L. were produced with stem explants that encapsulated in 4% of sodiumalginate supplemented with 1.0 mg/L BAP. In conclusion, this study was successively establishedan alternative way in propagating Ficus carica L. through plant tissue culture system. Theimplication of this study would able to help in give a good information to public and improvingagriculturalsector with new technologies. |
|---|