Effects of growth regulators on Plant Tissue Culture of Ficus carica L.

Ficus carica L. is one of Moraceae family which natively to Westerns Asia anddistributed worldwide and economically important especially in sericulture, horticulture, food andcosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins. Thisplant also produces secondary...

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Main Author:	Fadhlul Khaliq Ab Patah
Format:	thesis
Language:	eng
Published:	2020
Subjects:	Q Science
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=6875
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institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	Q Science
spellingShingle	Q Science Fadhlul Khaliq Ab Patah Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
description	Ficus carica L. is one of Moraceae family which natively to Westerns Asia anddistributed worldwide and economically important especially in sericulture, horticulture, food andcosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins. Thisplant also produces secondary metabolites, latex cells. The richness of nutrients andlatex cells make this plant possessed many pharmacological effects which is importantly tomedicinal and drugs industries. The demand for this plant is very high despite theproduction of fig is very inconsistent from year to year. So, this study aims to establish anddetermine the optimum concentration of benzylaminopurine (BAP) and napthelene acetic acid(NAA) for Ficus carica L. through plant tissue culture system by following completely randomizeddesign. The shoots were able to be regenerated from stems explants at most optimum concentrationof 1.0 mg/L BAP. For callus induction, all stem, shoot, petiole, and root explants able to emergecalli when cultured in MS medium with optimum combinations of BAP and NAA; 1.0 mg/L BAP +0.5 mg/L NAA, 0.5 mg/L BAP + 1.0 mg/L NAA, 1.5 mg/L BAP + 1.0 mg/L NAA, and 1.0 mg/L BAP + 1.0mg/L NAA respectively. The explants also were tested with other plant growth regulators such as thidiazuron (TDZ), Kinetin, indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) for a comparison. The results still showed that BAP and NAA werethe best plant growth regulators used compared to others combinations. The micromorphology studyshows the presence of trichomes on adaxial and abaxial of in vitro and in vivo leaves. Stoma wasalso visible on all surfaces except on adaxial of in vivo leaf. The acclimatization process allowedthe plantlets survived in garden soil with 86.67% after weeks 8 cultured. Lastly, synthetic seedsof Ficus carica L. were produced with stem explants that encapsulated in 4% of sodiumalginate supplemented with 1.0 mg/L BAP. In conclusion, this study was successively establishedan alternative way in propagating Ficus carica L. through plant tissue culture system. Theimplication of this study would able to help in give a good information to public and improvingagriculturalsector with new technologies.
format	thesis
qualification_name
qualification_level	Master's degree
author	Fadhlul Khaliq Ab Patah
author_facet	Fadhlul Khaliq Ab Patah
author_sort	Fadhlul Khaliq Ab Patah
title	Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
title_short	Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
title_full	Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
title_fullStr	Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
title_full_unstemmed	Effects of growth regulators on Plant Tissue Culture of Ficus carica L.
title_sort	effects of growth regulators on plant tissue culture of ficus carica l.
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Teknikal dan Vokasional
publishDate	2020
url	https://ir.upsi.edu.my/detailsg.php?det=6875
_version_	1747833324906217472
spelling	oai:ir.upsi.edu.my:68752022-03-16 Effects of growth regulators on Plant Tissue Culture of Ficus carica L. 2020 Fadhlul Khaliq Ab Patah Q Science Ficus carica L. is one of Moraceae family which natively to Westerns Asia anddistributed worldwide and economically important especially in sericulture, horticulture, food andcosmetic industries. Ficus carica L. contain numerous of nutrients, minerals and vitamins. Thisplant also produces secondary metabolites, latex cells. The richness of nutrients andlatex cells make this plant possessed many pharmacological effects which is importantly tomedicinal and drugs industries. The demand for this plant is very high despite theproduction of fig is very inconsistent from year to year. So, this study aims to establish anddetermine the optimum concentration of benzylaminopurine (BAP) and napthelene acetic acid(NAA) for Ficus carica L. through plant tissue culture system by following completely randomizeddesign. The shoots were able to be regenerated from stems explants at most optimum concentrationof 1.0 mg/L BAP. For callus induction, all stem, shoot, petiole, and root explants able to emergecalli when cultured in MS medium with optimum combinations of BAP and NAA; 1.0 mg/L BAP +0.5 mg/L NAA, 0.5 mg/L BAP + 1.0 mg/L NAA, 1.5 mg/L BAP + 1.0 mg/L NAA, and 1.0 mg/L BAP + 1.0mg/L NAA respectively. The explants also were tested with other plant growth regulators such as thidiazuron (TDZ), Kinetin, indole-3-butyric acid (IBA), and 2,4-dichlorophenoxyacetic acid (2,4-D) for a comparison. The results still showed that BAP and NAA werethe best plant growth regulators used compared to others combinations. The micromorphology studyshows the presence of trichomes on adaxial and abaxial of in vitro and in vivo leaves. Stoma wasalso visible on all surfaces except on adaxial of in vivo leaf. The acclimatization process allowedthe plantlets survived in garden soil with 86.67% after weeks 8 cultured. Lastly, synthetic seedsof Ficus carica L. were produced with stem explants that encapsulated in 4% of sodiumalginate supplemented with 1.0 mg/L BAP. In conclusion, this study was successively establishedan alternative way in propagating Ficus carica L. through plant tissue culture system. Theimplication of this study would able to help in give a good information to public and improvingagriculturalsector with new technologies. 2020 thesis https://ir.upsi.edu.my/detailsg.php?det=6875 https://ir.upsi.edu.my/detailsg.php?det=6875 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Teknikal dan Vokasional Abbasi, A. M., Khan, M. A., Khan, N., and Shah, M. H. (2013) Ethnobotanical survey ofmedicinally important wild edible fruits species used by tribal communities of LesserHimalayas-Pakistan. Journal of Ethnopharmacology, 148, 538-536.A. I. M. Saad, and A. M. Elshahed, (2012). Plant Tissue Culture Media. In RecentAdvance in Plant in vitro Culture. 2938.Al-Snafi, A. E. 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Effects of growth regulators on Plant Tissue Culture of Ficus carica L.

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