Morphogenesis studies of Brassica oleraceae L.var Capitata through tissue culture system
<p>Experiment were carried out to establish the most optimum culture condition and to</p><p>identify the most responsive explants for regeneration Brassica oleraceae L. var</p><p>Capitata through plant tissue culture system. Accli...
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| Format: | thesis |
| Language: | eng |
| Published: |
2021
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=7161 |
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| Summary: | <p>Experiment were carried out to establish the most optimum culture condition and to</p><p>identify the most responsive explants for regeneration Brassica oleraceae L. var</p><p>Capitata through plant tissue culture system. Acclimatization process for established</p><p>plantlets was also conducted. The experimental design of this study was Complete</p><p>Randomized Design (CRD) method. For shoot regeneration using different</p><p>concentrations of BAP and NAA, the most responsive explant was stem explants in</p><p>which MS medium supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA produced</p><p>8.400 0.889 shoots per explant. Whereas, the combination auxin and cytokinin also</p><p>showed stem explants was the most responsive. Subsequently, for root regeneration</p><p>using different concentrations of BAP and NAA, the most responsive explant was</p><p>leave in which MS media supplemented with 0.5 mg/L BAP and 0.5 mg/L NAA</p><p>produced 51.133 2.929 roots per explant. While the combination of auxin and</p><p>cytokinin showed the most responsive was root. Next, for callus induction with</p><p>combination of BAP and NAA showed that petiole was the most responsive explant.</p><p>Callus was white in colour with friable structure. Meanwhile, for callus induction with</p><p>combination of auxin and cytokinin showed stem was the most responsive. Callus was</p><p>light brown and friable. In addition, synthetic seeds were produced when microshoots</p><p>were encapsulated with 4.0% sodium alginate solution added with 1.0 mg/L BAP and</p><p>0.5 mg/L NAA. The germination rate was 6.832 0.622 shoots per explant. Finally,</p><p>the acclimatization of this plantlet succeeded with combination of (black soil and red</p><p>soil at 2:1 ratio) with the survival rate of 86.67%. This research showed that</p><p>morphogenesis studies of Brassica oleraceae L. var Capitata through tissue culture</p><p>system was successfully achieved. Further studies on chemical such as secondary</p><p>metabolites contained in the callus and explants extract should be carried out.</p> |
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