Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)

Phenotyping of human muscle based on its profile of myosin heavy chain isoforms is commonly used to help understand changes in muscle function. However, in many instances, measureable changes in force output or contractility occur in the absence of any change in myosin heavy chain profile. Therefore...

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Main Author:	Zulezwan Ab. Malik
Format:	thesis
Language:	eng
Published:	2015
Subjects:	QC Physics
Online Access:	https://ir.upsi.edu.my/detailsg.php?det=772
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spelling	oai:ir.upsi.edu.my:7722020-02-27 Proteomic analysis of diurnal variation in human skeletal muscle performance (IR) 2015 Zulezwan Ab. Malik QC Physics Phenotyping of human muscle based on its profile of myosin heavy chain isoforms is commonly used to help understand changes in muscle function. However, in many instances, measureable changes in force output or contractility occur in the absence of any change in myosin heavy chain profile. Therefore, more sophisticated analysis is required. Proteomic techniques including 2-dimensional gel electrophoresis, highperformance liquid chromatography and peptide mass spectrometry can be used to investigate changes in the abundance of hundreds of proteins simultaneously. To date, such techniques have not been used to specifically characterise the human myofibrillar proteome, or study how the myofibrillar proteome relates to muscle outputs such as peak isometric force or the velocity of contraction. This thesis presents a series of studies that develop proteomic techniques for the analysis of myofibrillar proteins as well as optimisation of techniques for measuring the range of muscle output from isometric through to velocity maximum of the human knee extensor muscles in vivo. After optimisation, the proteomic and muscle function measurement were employed to study diurnal variation. Time-of-day differences in sports performance and muscle function are widely reported, and typically, performance is 2015 thesis https://ir.upsi.edu.my/detailsg.php?det=772 https://ir.upsi.edu.my/detailsg.php?det=772 text eng closedAccess Doctoral Universiti Pendidikan Sultan Idris Fakulti Sains Sukan dan Kejurulatihan N/A
institution	Universiti Pendidikan Sultan Idris
collection	UPSI Digital Repository
language	eng
topic	QC Physics
spellingShingle	QC Physics Zulezwan Ab. Malik Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)
description	Phenotyping of human muscle based on its profile of myosin heavy chain isoforms is commonly used to help understand changes in muscle function. However, in many instances, measureable changes in force output or contractility occur in the absence of any change in myosin heavy chain profile. Therefore, more sophisticated analysis is required. Proteomic techniques including 2-dimensional gel electrophoresis, highperformance liquid chromatography and peptide mass spectrometry can be used to investigate changes in the abundance of hundreds of proteins simultaneously. To date, such techniques have not been used to specifically characterise the human myofibrillar proteome, or study how the myofibrillar proteome relates to muscle outputs such as peak isometric force or the velocity of contraction. This thesis presents a series of studies that develop proteomic techniques for the analysis of myofibrillar proteins as well as optimisation of techniques for measuring the range of muscle output from isometric through to velocity maximum of the human knee extensor muscles in vivo. After optimisation, the proteomic and muscle function measurement were employed to study diurnal variation. Time-of-day differences in sports performance and muscle function are widely reported, and typically, performance is
format	thesis
qualification_name
qualification_level	Doctorate
author	Zulezwan Ab. Malik
author_facet	Zulezwan Ab. Malik
author_sort	Zulezwan Ab. Malik
title	Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)
title_short	Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)
title_full	Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)
title_fullStr	Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)
title_full_unstemmed	Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)
title_sort	proteomic analysis of diurnal variation in human skeletal muscle performance (ir)
granting_institution	Universiti Pendidikan Sultan Idris
granting_department	Fakulti Sains Sukan dan Kejurulatihan
publishDate	2015
url	https://ir.upsi.edu.my/detailsg.php?det=772
_version_	1747832910699823104

Proteomic analysis of diurnal variation in human skeletal muscle performance (IR)

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