Phytochemicals and biological activities of Aquilaria subintegra Ding Hou
<p>This study aims to investigate the phytochemicals and biological activities from the</p><p>bark and leaf of Aquilaria subintegra Ding Hou. The samples were air-dried, ground, and gradient</p><p>extracted by cold extraction usin...
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| Format: | thesis |
| Language: | eng |
| Published: |
2020
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| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=9043 |
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| Summary: | <p>This study aims to investigate the phytochemicals and biological activities from the</p><p>bark and leaf of Aquilaria subintegra Ding Hou. The samples were air-dried, ground, and gradient</p><p>extracted by cold extraction using n-hexane, dichloromethane, and methanol successively. The bark</p><p>and leaf crude extracts were purified via chromatographic techniques to produce pure</p><p>compounds. The chemical structure of compounds were determined using spectroscopic methods such as</p><p>nuclear magnetic resonance, ultraviolet, infrared, mass spectrometry and comparison from the</p><p>literature review. The crude extracts and isolated compounds were tested for their anti-lipase,</p><p>antioxidant and anticancer activities through colorimetric test, DPPH free radical scavenging and</p><p>cytotoxicity assay, respectively. This study has successfully afforded 22 compounds, including</p><p>three new compounds, namely aquilene A, aquilene B, and subintegranol. Other compounds were</p><p>5-hydroxy-7,4-dimethoxyflavone, luteolin- 7,3,4-trimethyl ether,</p><p>5,3-dihydroxy-7,4-dimethoxyflavone, 7,3-dimethoxyluteolin, 5,7-dihydroxy-4-methoxyflavone,</p><p>-sitosterol, -sitostenone, stigmasterol, friedelin, epifriedelanol, phytol, and hentriacontane.</p><p>Anti-lipase activity of n-hexane extracts of leaf and methanol extracts of bark showed the highest</p><p>inhibition, 46% and 52% compared with the control, respectively. Meanwhile, through computational</p><p>study of 5-hydroxy-7,4-dimethoxyflavone exhibited the highest inhibition and predicted as a</p><p>competitive lipase inhibitor. Besides, the leaf of methanolic extract showed the strongest DPPH</p><p>radical scavenger with IC50 value of 29.56 6.37 g/mL. However, the tested isolated compounds</p><p>showed less ability in antioxidant activity. In cytotoxic activity, the leaf and bark of methanolic</p><p>extracts exhibited highest anticancer activity with IC50 values of 24.30 0.07 and 17.46 0.08</p><p>g/mL, respectively. Besides, 5- hydroxy-7,4-dimethoxyflavone showed the highest cytotoxicity</p><p>activity with IC50 value of 38.48 0.06 g/mL. In conclusion, this study successfully revealed</p><p>the presence of various phytochemicals in A. subintegra and their potential as anti-lipase,</p><p>antioxidant and anticancer agents. In implication, this study enhances knowledge on the</p><p>diversity of chemical compounds and their potential in modern medicine.</p><p></p> |
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