Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
<p>The study was conducted to investigate the acute toxicity of aqueous extract from Aquilaria</p><p>malaccensis (AMAE) and Aquilaria subintegra (ASAE) leaves and their histological effects</p><p>on several organ in ICR mice. Comp...
Saved in:
| Main Author: | |
|---|---|
| Format: | thesis |
| Language: | eng |
| Published: |
2022
|
| Subjects: | |
| Online Access: | https://ir.upsi.edu.my/detailsg.php?det=9267 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <p>The study was conducted to investigate the acute toxicity of aqueous extract from Aquilaria</p><p>malaccensis (AMAE) and Aquilaria subintegra (ASAE) leaves and their histological effects</p><p>on several organ in ICR mice. Comparison between both extracts was included in order to</p><p>determine which toxicity is more potent. A total of 156 ICR mice was used in this study</p><p>consisted of 78 male and 78 female with the age of 12 weeks old. The mice were divided</p><p>into 13 groups containing 12 mice (6 males and 6 females) in each group. 12 groups orally</p><p>received one type of extract either AMAE or ASAE with only one type concentration. The</p><p>concentrations used were 4000, 5000, 6000, 7000, 8000, 9000 mg/kg body weight. One</p><p>group on the other hand act as the control and treated with distilled water. Extract</p><p>administration was conducted only once on the first day. Mice body weight and organ weight</p><p>were compared and analyzed using one-way ANOVA and evaluation of abnormalities in</p><p>term of appearance and behavioral were conducted. After the 14th day, mice undergone</p><p>euthanisation approved by UPSI ethics committee (reference number: 2021-0002-03) in</p><p>order to conduct histological analysis on their organs which were liver, kidney and stomach.</p><p>Evaluation on their cellular structure were also conducted. Results showed significant</p><p>differences in both AMAE and ASAE male mice body weight. AMAE also showed higher</p><p>significant difference when compared with ASAE using t-test. On top of that, all treated mice</p><p>displayed drowsiness and their fur seems to be affected, primarily AMAE and ASAE female</p><p>mice face fur which appeared to be balding. Faeces condition in some AMAE treatment</p><p>groups also showed abnormalities. In terms of histological evaluation, mice liver, kidney and</p><p>stomach tissues were also affected. Some livers portal vein and sinusoids in AMAE</p><p>treatment were filled with blood. All treated mice glomerulus expanded while some</p><p>glomeruli burst in AMAE treatment. All treated mice mucosa length reduced and some</p><p>gastric glands in AMAE treatment showed abnormal changes. Therefore, it can be stated that</p><p>both Aquilaria malaccensis and Aquilaria subintegra is toxic due to the adverse effects it</p><p>caused on mice appearance, behaviour and organ tissues. Furthermore, Aquilaria</p><p>malaccensis showed more adverse reactions compared to Aquilaria subintegra indicating</p><p>higher toxicity. This implicates that both Aquilaria malaccensis and Aquilaria subintegra</p><p>can be toxic and unsafe if being use in a higher concentration.</p> |
|---|