Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice

Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice

<p>The study was conducted to investigate the acute toxicity of aqueous extract from Aquilaria</p><p>malaccensis (AMAE) and Aquilaria subintegra (ASAE) leaves and their histological effects</p><p>on several organ in ICR mice. Comp...

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Main Author: Azam Muzafar Ahmad Mokhtar
Format: thesis
Language:eng
Published: 2022
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QH Natural history
Online Access:https://ir.upsi.edu.my/detailsg.php?det=9267
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institution Universiti Pendidikan Sultan Idris
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topic QH Natural history
spellingShingle QH Natural history
Azam Muzafar Ahmad Mokhtar
Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
description <p>The study was conducted to investigate the acute toxicity of aqueous extract from Aquilaria</p><p>malaccensis (AMAE) and Aquilaria subintegra (ASAE) leaves and their histological effects</p><p>on several organ in ICR mice. Comparison between both extracts was included in order to</p><p>determine which toxicity is more potent. A total of 156 ICR mice was used in this study</p><p>consisted of 78 male and 78 female with the age of 12 weeks old. The mice were divided</p><p>into 13 groups containing 12 mice (6 males and 6 females) in each group. 12 groups orally</p><p>received one type of extract either AMAE or ASAE with only one type concentration. The</p><p>concentrations used were 4000, 5000, 6000, 7000, 8000, 9000 mg/kg body weight. One</p><p>group on the other hand act as the control and treated with distilled water. Extract</p><p>administration was conducted only once on the first day. Mice body weight and organ weight</p><p>were compared and analyzed using one-way ANOVA and evaluation of abnormalities in</p><p>term of appearance and behavioral were conducted. After the 14th day, mice undergone</p><p>euthanisation approved by UPSI ethics committee (reference number: 2021-0002-03) in</p><p>order to conduct histological analysis on their organs which were liver, kidney and stomach.</p><p>Evaluation on their cellular structure were also conducted. Results showed significant</p><p>differences in both AMAE and ASAE male mice body weight. AMAE also showed higher</p><p>significant difference when compared with ASAE using t-test. On top of that, all treated mice</p><p>displayed drowsiness and their fur seems to be affected, primarily AMAE and ASAE female</p><p>mice face fur which appeared to be balding. Faeces condition in some AMAE treatment</p><p>groups also showed abnormalities. In terms of histological evaluation, mice liver, kidney and</p><p>stomach tissues were also affected. Some livers portal vein and sinusoids in AMAE</p><p>treatment were filled with blood. All treated mice glomerulus expanded while some</p><p>glomeruli burst in AMAE treatment. All treated mice mucosa length reduced and some</p><p>gastric glands in AMAE treatment showed abnormal changes. Therefore, it can be stated that</p><p>both Aquilaria malaccensis and Aquilaria subintegra is toxic due to the adverse effects it</p><p>caused on mice appearance, behaviour and organ tissues. Furthermore, Aquilaria</p><p>malaccensis showed more adverse reactions compared to Aquilaria subintegra indicating</p><p>higher toxicity. This implicates that both Aquilaria malaccensis and Aquilaria subintegra</p><p>can be toxic and unsafe if being use in a higher concentration.</p>
format thesis
qualification_name
qualification_level Master's degree
author Azam Muzafar Ahmad Mokhtar
author_facet Azam Muzafar Ahmad Mokhtar
author_sort Azam Muzafar Ahmad Mokhtar
title Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
title_short Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
title_full Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
title_fullStr Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
title_full_unstemmed Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice
title_sort histological effects of aqueous extract from aquilaria malaccensis and aquilaria subintegra towards icr mice
granting_institution Universiti Pendidikan Sultan Idris
granting_department Fakulti Sains dan Matematik
publishDate 2022
url https://ir.upsi.edu.my/detailsg.php?det=9267
_version_ 1776104609409925120
spelling oai:ir.upsi.edu.my:92672023-08-02 Histological effects of Aqueous extract from Aquilaria malaccensis and Aquilaria subintegra towards ICR mice 2022 Azam Muzafar Ahmad Mokhtar QH Natural history <p>The study was conducted to investigate the acute toxicity of aqueous extract from Aquilaria</p><p>malaccensis (AMAE) and Aquilaria subintegra (ASAE) leaves and their histological effects</p><p>on several organ in ICR mice. Comparison between both extracts was included in order to</p><p>determine which toxicity is more potent. A total of 156 ICR mice was used in this study</p><p>consisted of 78 male and 78 female with the age of 12 weeks old. The mice were divided</p><p>into 13 groups containing 12 mice (6 males and 6 females) in each group. 12 groups orally</p><p>received one type of extract either AMAE or ASAE with only one type concentration. The</p><p>concentrations used were 4000, 5000, 6000, 7000, 8000, 9000 mg/kg body weight. One</p><p>group on the other hand act as the control and treated with distilled water. Extract</p><p>administration was conducted only once on the first day. Mice body weight and organ weight</p><p>were compared and analyzed using one-way ANOVA and evaluation of abnormalities in</p><p>term of appearance and behavioral were conducted. After the 14th day, mice undergone</p><p>euthanisation approved by UPSI ethics committee (reference number: 2021-0002-03) in</p><p>order to conduct histological analysis on their organs which were liver, kidney and stomach.</p><p>Evaluation on their cellular structure were also conducted. Results showed significant</p><p>differences in both AMAE and ASAE male mice body weight. AMAE also showed higher</p><p>significant difference when compared with ASAE using t-test. On top of that, all treated mice</p><p>displayed drowsiness and their fur seems to be affected, primarily AMAE and ASAE female</p><p>mice face fur which appeared to be balding. Faeces condition in some AMAE treatment</p><p>groups also showed abnormalities. In terms of histological evaluation, mice liver, kidney and</p><p>stomach tissues were also affected. Some livers portal vein and sinusoids in AMAE</p><p>treatment were filled with blood. All treated mice glomerulus expanded while some</p><p>glomeruli burst in AMAE treatment. All treated mice mucosa length reduced and some</p><p>gastric glands in AMAE treatment showed abnormal changes. Therefore, it can be stated that</p><p>both Aquilaria malaccensis and Aquilaria subintegra is toxic due to the adverse effects it</p><p>caused on mice appearance, behaviour and organ tissues. Furthermore, Aquilaria</p><p>malaccensis showed more adverse reactions compared to Aquilaria subintegra indicating</p><p>higher toxicity. This implicates that both Aquilaria malaccensis and Aquilaria subintegra</p><p>can be toxic and unsafe if being use in a higher concentration.</p> 2022 thesis https://ir.upsi.edu.my/detailsg.php?det=9267 https://ir.upsi.edu.my/detailsg.php?det=9267 text eng closedAccess Masters Universiti Pendidikan Sultan Idris Fakulti Sains dan Matematik <p>Abba S, Omotoso O Dare, & Faculty of Basic Medical Sciences, Department of Anatomy, College of Health Sciences, Kogi State University Anyigba, Nigeria. (2018). Hemorrhagic Centrolobar Necrosis and Cytoplasmic Vacuolation of the Hepatocytes in Syzygium Guineense Chronic Treated Mice. International Journal of Anatomy and Applied Physiology, 99102. https://doi.org/10.19070/2572-7451-1800018</p><p>Abdul Razak, R. N. H., Ismail, F., Md Isa, M. L., Abdul Wahab, A. Y., Muhammad, H., Ramli, R., & Raja Ismail, R. A. S. (2019). 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