Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar

Bisphenol A or BPA are used in the production of epoxy resins and polycarbonate plastics. Other applications of BPA are in baby bottles, canned food and drinks and polymers contained in dental treatment. BPA is a type of xenoestrogen that has been shown to mimic the actions of estrogen. Particularly...

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Main Author:	Anuar, Aizamin
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Pharmaceutical industry
Online Access:	https://ir.uitm.edu.my/id/eprint/107032/1/107032.PDF
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spelling	my-uitm-ir.1070322024-12-10T23:09:23Z Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar 2009 Anuar, Aizamin Pharmaceutical industry Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Bisphenol A or BPA are used in the production of epoxy resins and polycarbonate plastics. Other applications of BPA are in baby bottles, canned food and drinks and polymers contained in dental treatment. BPA is a type of xenoestrogen that has been shown to mimic the actions of estrogen. Particularly in females, BPA causes ovarian disease, stimulation of mammary gland, onset of sexual maturation and disruption of the estrous cycle. Other than that, BPA can also stimulate human breast cancer cell. The dose and the effects of BPA on the reproductive system vary. The effects of BPA on the early onset of sexual maturation in females was seen at 2.4 and 500µg/kg/day, the stimulation of mammary gland in female offspring occurred at very low dose of 0.025µg/kg/day and BPA disrupts the adult estrous cycle at the dose between 100 and 500µg/kg/day. To determine the concentration of protein in female rats, we force-feed the rats with dose of BPA ranging from 50, 500, 1000 and 5000µg/kg/day and using SOS PAGE to observe the intensity of the concentration of protein in each sample. In our study, the concentration of proteins in the group of rats fed with 50 and 500µg/kg/day increased above the concentration of proteins in the negative control groups especially in the group with the 500µg/kg/day dose which was almost similar to concentrations detected in the positive control. However, at 1000µg/kg/day, there was almost no protein and at 5000µg/kg/day the concentration of protein was similar to the negative control group. Based on the weight of ovary, the negative control and the group fed with 1000 and 5000µg/kg/day had increased weight compared to the other groups which shows that a lower concentration of protein correlates with increased of ovary weight. However, further analysis using Western Blot needs to be carried out in the future so that the specific band and concentration for estrogen and progesterone could then be observed and analyzed. 2009 Thesis https://ir.uitm.edu.my/id/eprint/107032/ https://ir.uitm.edu.my/id/eprint/107032/1/107032.PDF text en public degree Universiti Teknologi MARA (Kampus Puncak Alam) Faculty of Pharmacy Mohd Mutalip, Siti Syairah
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Mohd Mutalip, Siti Syairah
topic	Pharmaceutical industry Pharmaceutical industry
spellingShingle	Pharmaceutical industry Pharmaceutical industry Anuar, Aizamin Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar
description	Bisphenol A or BPA are used in the production of epoxy resins and polycarbonate plastics. Other applications of BPA are in baby bottles, canned food and drinks and polymers contained in dental treatment. BPA is a type of xenoestrogen that has been shown to mimic the actions of estrogen. Particularly in females, BPA causes ovarian disease, stimulation of mammary gland, onset of sexual maturation and disruption of the estrous cycle. Other than that, BPA can also stimulate human breast cancer cell. The dose and the effects of BPA on the reproductive system vary. The effects of BPA on the early onset of sexual maturation in females was seen at 2.4 and 500µg/kg/day, the stimulation of mammary gland in female offspring occurred at very low dose of 0.025µg/kg/day and BPA disrupts the adult estrous cycle at the dose between 100 and 500µg/kg/day. To determine the concentration of protein in female rats, we force-feed the rats with dose of BPA ranging from 50, 500, 1000 and 5000µg/kg/day and using SOS PAGE to observe the intensity of the concentration of protein in each sample. In our study, the concentration of proteins in the group of rats fed with 50 and 500µg/kg/day increased above the concentration of proteins in the negative control groups especially in the group with the 500µg/kg/day dose which was almost similar to concentrations detected in the positive control. However, at 1000µg/kg/day, there was almost no protein and at 5000µg/kg/day the concentration of protein was similar to the negative control group. Based on the weight of ovary, the negative control and the group fed with 1000 and 5000µg/kg/day had increased weight compared to the other groups which shows that a lower concentration of protein correlates with increased of ovary weight. However, further analysis using Western Blot needs to be carried out in the future so that the specific band and concentration for estrogen and progesterone could then be observed and analyzed.
format	Thesis
qualification_level	Bachelor degree
author	Anuar, Aizamin
author_facet	Anuar, Aizamin
author_sort	Anuar, Aizamin
title	Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar
title_short	Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar
title_full	Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar
title_fullStr	Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar
title_full_unstemmed	Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar
title_sort	detection of protein changes in female reproductive system of dose response bisphenol a treated sd juvenile rats / aizamin anuar
granting_institution	Universiti Teknologi MARA (Kampus Puncak Alam)
granting_department	Faculty of Pharmacy
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/107032/1/107032.PDF
_version_	1818588202088267776

Detection of protein changes in female reproductive system of dose response bisphenol A treated SD juvenile rats / Aizamin Anuar

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