Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim

The encapsulation of multi-enzyme (alpha-amylase, glucoamylase and cellulase) within calcium alginate-clay beads was used in the saccharification of cassava into reducing sugar in a stirred tank bioreactor. The functional group, morphology and composition of beads were characterized by Fourier Tr...

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Main Author:	Abd Rahim, Siti Noraida
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Home arts Homecrafts.
Online Access:	https://ir.uitm.edu.my/id/eprint/21620/1/TM_SITI%20NORAIDA%20ABD%20RAHIM%20AT%2015_5.pdf
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spelling	my-uitm-ir.216202018-09-26T03:31:32Z Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim 2015 Abd Rahim, Siti Noraida Home arts. Homecrafts. The encapsulation of multi-enzyme (alpha-amylase, glucoamylase and cellulase) within calcium alginate-clay beads was used in the saccharification of cassava into reducing sugar in a stirred tank bioreactor. The functional group, morphology and composition of beads were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscope (FESEM) equipped with Energy Dispersive X-ray Spectroscopy (EDX). The optimal encapsulation conditions of multi-enzyme in alginate-clay beads were obtained at 2 % (w/v) clay concentration and 0.2 M CaCl2 solution which gave 97.34 % of loading efficiency and 53.71 % of immobilization yield. Kinetic parameters were determined for encapsulated multienzyme, as well as for the free multi-enzyme. The Michaelis constant, Km value for encapsulated multi-enzyme (4.9176 mg/mL) was 1.39 times higher than free multienzyme (3.5367 mg/mL), whereas the maximum reaction velocity, Vmax value was lower for the encapsulated multi-enzyme. Based on the one-factor-at-one-time (OFAT) study, the optimum pH, temperature and agitation speed for both free and encapsulated multi-enzyme were pH 6.0, 50 °C and 120 rpm, respectively. Furthermore, the optimum conditions for saccharification process were statistical determined using Response Surface Methodology (RSM). After screening significant factors that influence the saccharification process, pH, temperature and agitation speed were optimized by implementing central composite design (CCD) in RSM. The optimum values of pH, temperature and agitation speed were found to be pH 5.9, 44 °C and 94 rpm, respectively. Under these conditions, the experimental yield was 88.19 % which was close to the predicted yield (91.95 %). Meanwhile, encapsulated multienzyme was retained 15.09 % of its activity after 5 reaction cycles. 2015 Thesis https://ir.uitm.edu.my/id/eprint/21620/ https://ir.uitm.edu.my/id/eprint/21620/1/TM_SITI%20NORAIDA%20ABD%20RAHIM%20AT%2015_5.pdf text en public masters Universiti Teknologi MARA Faculty of Plantation and Agrotechnology
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
topic	Home arts Homecrafts.
spellingShingle	Home arts Homecrafts. Abd Rahim, Siti Noraida Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim
description	The encapsulation of multi-enzyme (alpha-amylase, glucoamylase and cellulase) within calcium alginate-clay beads was used in the saccharification of cassava into reducing sugar in a stirred tank bioreactor. The functional group, morphology and composition of beads were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscope (FESEM) equipped with Energy Dispersive X-ray Spectroscopy (EDX). The optimal encapsulation conditions of multi-enzyme in alginate-clay beads were obtained at 2 % (w/v) clay concentration and 0.2 M CaCl2 solution which gave 97.34 % of loading efficiency and 53.71 % of immobilization yield. Kinetic parameters were determined for encapsulated multienzyme, as well as for the free multi-enzyme. The Michaelis constant, Km value for encapsulated multi-enzyme (4.9176 mg/mL) was 1.39 times higher than free multienzyme (3.5367 mg/mL), whereas the maximum reaction velocity, Vmax value was lower for the encapsulated multi-enzyme. Based on the one-factor-at-one-time (OFAT) study, the optimum pH, temperature and agitation speed for both free and encapsulated multi-enzyme were pH 6.0, 50 °C and 120 rpm, respectively. Furthermore, the optimum conditions for saccharification process were statistical determined using Response Surface Methodology (RSM). After screening significant factors that influence the saccharification process, pH, temperature and agitation speed were optimized by implementing central composite design (CCD) in RSM. The optimum values of pH, temperature and agitation speed were found to be pH 5.9, 44 °C and 94 rpm, respectively. Under these conditions, the experimental yield was 88.19 % which was close to the predicted yield (91.95 %). Meanwhile, encapsulated multienzyme was retained 15.09 % of its activity after 5 reaction cycles.
format	Thesis
qualification_level	Master's degree
author	Abd Rahim, Siti Noraida
author_facet	Abd Rahim, Siti Noraida
author_sort	Abd Rahim, Siti Noraida
title	Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim
title_short	Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim
title_full	Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim
title_fullStr	Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim
title_full_unstemmed	Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim
title_sort	enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / siti noraida abd rahim
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Plantation and Agrotechnology
publishDate	2015
url	https://ir.uitm.edu.my/id/eprint/21620/1/TM_SITI%20NORAIDA%20ABD%20RAHIM%20AT%2015_5.pdf
_version_	1783733759295619072

Enhanced performance of encapsulated multi-enzyme in indigenous sayong clay for cassava saccharification process / Siti Noraida Abd Rahim

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