Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman

The chemical compositions of green mussel (Perna viridis) hydrolysate produced at different degree of hydrolysis were studied. Green mussel hydrolysate produced at pH 7 with E/S ratio 5% produced higher percentage yield with 37.00% but lower degree of hydrolysis (DH) with 28.33% as compared to that...

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Main Author: Azman, Nurulizzaira
Format: Thesis
Language:English
Published: 2012
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Online Access:https://ir.uitm.edu.my/id/eprint/53077/1/53077.pdf
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spelling my-uitm-ir.530772021-12-15T07:43:47Z Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman 2012 Azman, Nurulizzaira Physical and theoretical chemistry Extraction (Chemistry) The chemical compositions of green mussel (Perna viridis) hydrolysate produced at different degree of hydrolysis were studied. Green mussel hydrolysate produced at pH 7 with E/S ratio 5% produced higher percentage yield with 37.00% but lower degree of hydrolysis (DH) with 28.33% as compared to that produced at pH 9 with E/S ratio 3%. The DH is affected by the methods used to calculate DH and amount of sodium hydroxide added during hydrolysis. Chemical analyses including protein, moisture, fat and ash showed significance difference (p<0.050) between "the two conditions of green mussel hydrolysate with lower DH producing lower proximate value. Several amino acids associated with bitterness were found in high amount in green mussel hydrolysate produced at pH 9 with E/S ratio 3% such as histidine, tyrosine, valine, methionine, lysine, isoleucine, and phenyalanine. Hydrophobic group analysis by using FTIR detected the presence of amine group which contributed to the bitterness of hydrolysate produced in both conditions. Compounds found in raw green mussel (Perna viridis), mussel hydrolysate produced at pH 7 with E/S ratio 5% and pH 9 with E/S ratio 3% are saturated fatty acids, monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), aldehyde, ketone, amide and esters. Raw green mussel produced high amount of Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) which is not presence in green mussel hydrolysate. Hydrolysate produced at pH 7 with E/S ratio 5% has better characteristics that has the potential to be used as flavouring agent due to its less bitter characteristics compared to pH 9 with E/S ratio 3%. 2012 Thesis https://ir.uitm.edu.my/id/eprint/53077/ https://ir.uitm.edu.my/id/eprint/53077/1/53077.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution Universiti Teknologi MARA
collection UiTM Institutional Repository
language English
advisor Ismail, Normah (Dr.)
topic Physical and theoretical chemistry
Extraction (Chemistry)
spellingShingle Physical and theoretical chemistry
Extraction (Chemistry)
Azman, Nurulizzaira
Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman
description The chemical compositions of green mussel (Perna viridis) hydrolysate produced at different degree of hydrolysis were studied. Green mussel hydrolysate produced at pH 7 with E/S ratio 5% produced higher percentage yield with 37.00% but lower degree of hydrolysis (DH) with 28.33% as compared to that produced at pH 9 with E/S ratio 3%. The DH is affected by the methods used to calculate DH and amount of sodium hydroxide added during hydrolysis. Chemical analyses including protein, moisture, fat and ash showed significance difference (p<0.050) between "the two conditions of green mussel hydrolysate with lower DH producing lower proximate value. Several amino acids associated with bitterness were found in high amount in green mussel hydrolysate produced at pH 9 with E/S ratio 3% such as histidine, tyrosine, valine, methionine, lysine, isoleucine, and phenyalanine. Hydrophobic group analysis by using FTIR detected the presence of amine group which contributed to the bitterness of hydrolysate produced in both conditions. Compounds found in raw green mussel (Perna viridis), mussel hydrolysate produced at pH 7 with E/S ratio 5% and pH 9 with E/S ratio 3% are saturated fatty acids, monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), aldehyde, ketone, amide and esters. Raw green mussel produced high amount of Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) which is not presence in green mussel hydrolysate. Hydrolysate produced at pH 7 with E/S ratio 5% has better characteristics that has the potential to be used as flavouring agent due to its less bitter characteristics compared to pH 9 with E/S ratio 3%.
format Thesis
qualification_level Bachelor degree
author Azman, Nurulizzaira
author_facet Azman, Nurulizzaira
author_sort Azman, Nurulizzaira
title Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman
title_short Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman
title_full Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman
title_fullStr Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman
title_full_unstemmed Influence of degree of hydrolysis on the chemical Compositions of hydrolysate produced from green Mussel (Perna viridis) / Nurulizzaira Azman
title_sort influence of degree of hydrolysis on the chemical compositions of hydrolysate produced from green mussel (perna viridis) / nurulizzaira azman
granting_institution Universiti Teknologi MARA
granting_department Faculty of Applied Sciences
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/53077/1/53077.pdf
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