Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri

Fish protein hydrolysate was prepared from Sillago Banded Whitings fish (Sillago argentifasciata). Hydrolysis condition such as pH and enzyme substrate ratio was used for preparing fish protein hydrolysate which were optimised by response surface methodology (RSM) using a factorial design. Model equ...

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Main Author:	Mohd Sukeri, Nurul Asyikin
Format:	Thesis
Language:	English
Published:	2009
Subjects:	Aromatic compounds Analytical chemistry
Online Access:	https://ir.uitm.edu.my/id/eprint/53086/1/53086.pdf
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id	my-uitm-ir.53086
record_format	uketd_dc
spelling	my-uitm-ir.530862021-12-15T04:07:27Z Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri 2009 Mohd Sukeri, Nurul Asyikin Aromatic compounds Analytical chemistry Fish protein hydrolysate was prepared from Sillago Banded Whitings fish (Sillago argentifasciata). Hydrolysis condition such as pH and enzyme substrate ratio was used for preparing fish protein hydrolysate which were optimised by response surface methodology (RSM) using a factorial design. Model equation was proposed, with regard to the effect of pH and enzyme substrate ratio. The optimum percentage of nitrogen recovery (%NR) achieved was about 13.35% with optimum pH of 6.79 and optimum enzyme substrate ratio of 2.77% using alcalase. An enzyme substrate ratio of 0.33-% and pH of 9.00 were found to be the optimum conditions to obtain a high degree of hydrolysis close to 0.59% using alcalase. The hydrolysate prepared from Sillago banded whiting fish has potential for applications in aquaculture or animal feeds and also has a potential to be an effective nitrogenous source in microbiological growth media. Sillago banded whiting fish hydrolysate also has potential to be commercialise as a flavour enhancer. 2009 Thesis https://ir.uitm.edu.my/id/eprint/53086/ https://ir.uitm.edu.my/id/eprint/53086/1/53086.pdf text en public degree Universiti Teknologi MARA Faculty of Applied Sciences Ismail, Normah (Dr.)
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Ismail, Normah (Dr.)
topic	Aromatic compounds Analytical chemistry
spellingShingle	Aromatic compounds Analytical chemistry Mohd Sukeri, Nurul Asyikin Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri
description	Fish protein hydrolysate was prepared from Sillago Banded Whitings fish (Sillago argentifasciata). Hydrolysis condition such as pH and enzyme substrate ratio was used for preparing fish protein hydrolysate which were optimised by response surface methodology (RSM) using a factorial design. Model equation was proposed, with regard to the effect of pH and enzyme substrate ratio. The optimum percentage of nitrogen recovery (%NR) achieved was about 13.35% with optimum pH of 6.79 and optimum enzyme substrate ratio of 2.77% using alcalase. An enzyme substrate ratio of 0.33-% and pH of 9.00 were found to be the optimum conditions to obtain a high degree of hydrolysis close to 0.59% using alcalase. The hydrolysate prepared from Sillago banded whiting fish has potential for applications in aquaculture or animal feeds and also has a potential to be an effective nitrogenous source in microbiological growth media. Sillago banded whiting fish hydrolysate also has potential to be commercialise as a flavour enhancer.
format	Thesis
qualification_level	Bachelor degree
author	Mohd Sukeri, Nurul Asyikin
author_facet	Mohd Sukeri, Nurul Asyikin
author_sort	Mohd Sukeri, Nurul Asyikin
title	Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri
title_short	Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri
title_full	Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri
title_fullStr	Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri
title_full_unstemmed	Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri
title_sort	optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (sillago argentifasciata): effect of ph and enzyme substrate ratio / nurul asyikin mohd sukeri
granting_institution	Universiti Teknologi MARA
granting_department	Faculty of Applied Sciences
publishDate	2009
url	https://ir.uitm.edu.my/id/eprint/53086/1/53086.pdf
_version_	1783734861907886080

Optimisation of alcalase hydrolysis condition for preparing fish protein hydrolysate from sillago banded whitings (Sillago argentifasciata): effect of pH and enzyme substrate ratio / Nurul Asyikin Mohd Sukeri

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