Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud

The rising of resistances toward present antibiotics have long been reported by many physicians, and researchers have become concern by this issue. In order to keep searching novel drugs that will be available in the future, this project research aims to contribute in collecting data from the endoph...

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Main Author:	Mat Daud, Nor Izzati
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Pharmaceutical chemistry Microbial biotechnology
Online Access:	https://ir.uitm.edu.my/id/eprint/66741/1/66741.pdf
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id	my-uitm-ir.66741
record_format	uketd_dc
spelling	my-uitm-ir.667412022-09-15T00:24:15Z Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud 2012 Mat Daud, Nor Izzati Pharmaceutical chemistry Microbial biotechnology The rising of resistances toward present antibiotics have long been reported by many physicians, and researchers have become concern by this issue. In order to keep searching novel drugs that will be available in the future, this project research aims to contribute in collecting data from the endophytic fungi obtained from Malaysian environment (Puncak Alam forest) as part of a large drug discovery programme. The study was done to investigate the secondary metabolites produced by endophytic fungi TH2S14 and TH3R30 that can be used for medicinal plant purpose. The endophytic fungi were cultured on Potato Dextrose Agar Plate (PDA), and extracted using ethyl acetate as a solvent. Then, the fungal extracts had been subjected to analytical High performance liquid chromatography (HPLC) using a diode array detector (DAD) and proceed to Semi-preparative HPLC to collect the extract fractions. The antimicrobial activity was tested only to the fractions of fungus TH2S14 because of time constraint. From the antibacterial activity test by MTT assay, the extract fractions only showed positive result against Enterococcus faecium and Escherichia coli not to Staphylococcus aureus and Pseudomonas aeruginosa as stated in the reference. This may be due to the poor techniques of antibacterial activity test being conducted. 2012 Thesis https://ir.uitm.edu.my/id/eprint/66741/ https://ir.uitm.edu.my/id/eprint/66741/1/66741.pdf text en public degree Universiti Teknologi MARA (UiTM) Faculty of Pharmacy Sultan, Sadia Weber Abdullah, J.F.F. Ramasamy, Kalavathy Syed Adnan
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Sultan, Sadia Weber Abdullah, J.F.F. Ramasamy, Kalavathy Syed Adnan
topic	Pharmaceutical chemistry Microbial biotechnology
spellingShingle	Pharmaceutical chemistry Microbial biotechnology Mat Daud, Nor Izzati Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud
description	The rising of resistances toward present antibiotics have long been reported by many physicians, and researchers have become concern by this issue. In order to keep searching novel drugs that will be available in the future, this project research aims to contribute in collecting data from the endophytic fungi obtained from Malaysian environment (Puncak Alam forest) as part of a large drug discovery programme. The study was done to investigate the secondary metabolites produced by endophytic fungi TH2S14 and TH3R30 that can be used for medicinal plant purpose. The endophytic fungi were cultured on Potato Dextrose Agar Plate (PDA), and extracted using ethyl acetate as a solvent. Then, the fungal extracts had been subjected to analytical High performance liquid chromatography (HPLC) using a diode array detector (DAD) and proceed to Semi-preparative HPLC to collect the extract fractions. The antimicrobial activity was tested only to the fractions of fungus TH2S14 because of time constraint. From the antibacterial activity test by MTT assay, the extract fractions only showed positive result against Enterococcus faecium and Escherichia coli not to Staphylococcus aureus and Pseudomonas aeruginosa as stated in the reference. This may be due to the poor techniques of antibacterial activity test being conducted.
format	Thesis
qualification_level	Bachelor degree
author	Mat Daud, Nor Izzati
author_facet	Mat Daud, Nor Izzati
author_sort	Mat Daud, Nor Izzati
title	Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud
title_short	Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud
title_full	Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud
title_fullStr	Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud
title_full_unstemmed	Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud
title_sort	antimicrobial activities of endophytic fungi metabolites / nor izzati mat daud
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Pharmacy
publishDate	2012
url	https://ir.uitm.edu.my/id/eprint/66741/1/66741.pdf
_version_	1783735635937329152

Antimicrobial activities of endophytic fungi metabolites / Nor Izzati Mat Daud

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