Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari

Salmonellosis is a foodborne disease caused by Salmonella typhimurium; a Gram-negative bacterium that can form biofilm in infected hosts. The effectiveness of existing commercial disinfectants against Salmonella biofilm especially on biomass, viability and biochemical composition of S. typhimurium b...

Full description

Saved in:

Bibliographic Details
Main Author:	Johari, Nur Anisah
Format:	Thesis
Language:	English
Published:	2022
Subjects:	QR Microbiology
Online Access:	https://ir.uitm.edu.my/id/eprint/72941/1/72941.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uitm-ir.72941
record_format	uketd_dc
spelling	my-uitm-ir.729412023-02-01T13:54:12Z Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari 2022 Johari, Nur Anisah QR Microbiology Public health. Hygiene. Preventive Medicine Salmonellosis is a foodborne disease caused by Salmonella typhimurium; a Gram-negative bacterium that can form biofilm in infected hosts. The effectiveness of existing commercial disinfectants against Salmonella biofilm especially on biomass, viability and biochemical composition of S. typhimurium biofilm needs to be further investigated for biofilm control. The objective of this study was to evaluate the effects of commercial disinfectants against biomass, viability and biochemical composition of S. typhimurium biofilm. S. typhimurium ATCC14028 biofilm was developed in the absence and presence of commercial disinfectants. Sodium hypochlorite, sodium dodecyl-benzene sulfonate, benzalkonium chloride and chloroxylenol-based disinfectants were evaluated in the range between 0.78% and 25%. Biofilm biomass, biofilm viability and biofilm biochemical composition were determined using crystal violet assay, resazurin assay and FTIR-ATR spectroscopy respectively. Results demonstrated that all commercial disinfectants effectively inhibited S. typhimurium biofilm. Treatment with chloroxylenol‐based disinfectant significantly (p<0.05) reduced biofilm biomass and biofilm viability at all tested concentrations. Correlation between biofilm biomass and biofilm viability in the presence of chloroxylenol‐based disinfectant was also found to be significant with r=0.857 and its half-maximal biofilm inhibitory concentration (BIC50) showed the lowest values of 5.06%. Biochemical composition of S. typhimurium biofilm treated with chloroxylenol‐based disinfectant showed changes in FTIR spectral peaks in lipid (1460 cm−1,1400 cm−1) and protein (1550 cm−1,1460 cm−1,1400 cm−1). The findings of the present study suggest that the most efficient agent against S. typhimurium biofilm is a chloroxylenol‐based disinfectant. 2022 Thesis https://ir.uitm.edu.my/id/eprint/72941/ https://ir.uitm.edu.my/id/eprint/72941/1/72941.pdf text en public masters Universiti Teknologi MARA (UiTM) Faculty of Applied Sciences Raja Yahya, Mohd Fakharul Zaman
institution	Universiti Teknologi MARA
collection	UiTM Institutional Repository
language	English
advisor	Raja Yahya, Mohd Fakharul Zaman
topic	QR Microbiology QR Microbiology
spellingShingle	QR Microbiology QR Microbiology Johari, Nur Anisah Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari
description	Salmonellosis is a foodborne disease caused by Salmonella typhimurium; a Gram-negative bacterium that can form biofilm in infected hosts. The effectiveness of existing commercial disinfectants against Salmonella biofilm especially on biomass, viability and biochemical composition of S. typhimurium biofilm needs to be further investigated for biofilm control. The objective of this study was to evaluate the effects of commercial disinfectants against biomass, viability and biochemical composition of S. typhimurium biofilm. S. typhimurium ATCC14028 biofilm was developed in the absence and presence of commercial disinfectants. Sodium hypochlorite, sodium dodecyl-benzene sulfonate, benzalkonium chloride and chloroxylenol-based disinfectants were evaluated in the range between 0.78% and 25%. Biofilm biomass, biofilm viability and biofilm biochemical composition were determined using crystal violet assay, resazurin assay and FTIR-ATR spectroscopy respectively. Results demonstrated that all commercial disinfectants effectively inhibited S. typhimurium biofilm. Treatment with chloroxylenol‐based disinfectant significantly (p<0.05) reduced biofilm biomass and biofilm viability at all tested concentrations. Correlation between biofilm biomass and biofilm viability in the presence of chloroxylenol‐based disinfectant was also found to be significant with r=0.857 and its half-maximal biofilm inhibitory concentration (BIC50) showed the lowest values of 5.06%. Biochemical composition of S. typhimurium biofilm treated with chloroxylenol‐based disinfectant showed changes in FTIR spectral peaks in lipid (1460 cm−1,1400 cm−1) and protein (1550 cm−1,1460 cm−1,1400 cm−1). The findings of the present study suggest that the most efficient agent against S. typhimurium biofilm is a chloroxylenol‐based disinfectant.
format	Thesis
qualification_level	Master's degree
author	Johari, Nur Anisah
author_facet	Johari, Nur Anisah
author_sort	Johari, Nur Anisah
title	Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari
title_short	Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari
title_full	Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari
title_fullStr	Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari
title_full_unstemmed	Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari
title_sort	inhibition of salmonella typhimurium biofilm by commercial disinfectants / nur anisah johari
granting_institution	Universiti Teknologi MARA (UiTM)
granting_department	Faculty of Applied Sciences
publishDate	2022
url	https://ir.uitm.edu.my/id/eprint/72941/1/72941.pdf
_version_	1783735963474722816

Inhibition of Salmonella typhimurium biofilm by commercial disinfectants / Nur Anisah Johari

Similar Items