Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste

Fructooligosaccharides (FOS), which are considered as inulin-type fructans, represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. Prebiotics are considered as additives of functional food due to the ability to promote host health by increasin...

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Main Author:	Nur Dini, Mat Jusoh
Format:	Thesis
Language:	English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/31171/1/Production%20of%20fructooligosaccharides%20%28FOS%29%20by%20enzymatic%20reaction%20from%20phytoenzyme.pdf
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spelling	my-ump-ir.311712021-04-15T03:39:44Z Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste 2019-12 Nur Dini, Mat Jusoh TP Chemical technology Fructooligosaccharides (FOS), which are considered as inulin-type fructans, represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. Prebiotics are considered as additives of functional food due to the ability to promote host health by increasing the growth of probiotic microorganisms. In this study, FOS were produced by using enzymatic reaction of the phytoenzymes from Ananas Comosus waste.The characterisation of invertase and the phytoenzymes of A. Comosus waste was performed prior to FOS production by using Fourier transfrom infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), and ultraviolet-visible (UV-Vis) spectroscopy. FOS obtained from phytoenzymes and invertase showed similarities in terms of complex protein compounds. FTIR results showed that both invertase and the phytoenzymes from A.comosus waste give stretching from 1640.60 cm-1 and 1649.99cm-1 respectively and it is N-H bond. The chromatographic profile of the reaction product from the phytoenzyme of A.comosus waste revealed the presence of sucrose, fructose, glucose and FOS namely, kestose, and nystose. The production of FOS proceeds by fractional factorial design (FFD) central composite design (CCD) statistical methods with the optimisation parameters of reaction time, sucrose concentration, pH, phytoenzyme concentration and temperature using Design Expert version 7.0. All of these responses were screened by FFD method which showed that all the parameters were significant response to produce FOS. CCD method evaluates that pH (6) and temperature 60˚C was producing maximum FOS at 211.334(g/mL). The validation of empirical equations gained from the optimisation study was performed in laboratory scale with only 0.828 % error. As a conclusion, this study has successfully produced FOS using a new approach of alternative source which is from the phytoenzymes of A.Comosus waste. 2019-12 Thesis http://umpir.ump.edu.my/id/eprint/31171/ http://umpir.ump.edu.my/id/eprint/31171/1/Production%20of%20fructooligosaccharides%20%28FOS%29%20by%20enzymatic%20reaction%20from%20phytoenzyme.pdf pdf en public masters Universiti Malaysia Pahang Faculty of Chemical and Process Engineering Technology
institution	Universiti Malaysia Pahang Al-Sultan Abdullah
collection	UMPSA Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur Dini, Mat Jusoh Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste
description	Fructooligosaccharides (FOS), which are considered as inulin-type fructans, represent an important source of prebiotic compounds that are widely used as an ingredient in functional foods. Prebiotics are considered as additives of functional food due to the ability to promote host health by increasing the growth of probiotic microorganisms. In this study, FOS were produced by using enzymatic reaction of the phytoenzymes from Ananas Comosus waste.The characterisation of invertase and the phytoenzymes of A. Comosus waste was performed prior to FOS production by using Fourier transfrom infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), and ultraviolet-visible (UV-Vis) spectroscopy. FOS obtained from phytoenzymes and invertase showed similarities in terms of complex protein compounds. FTIR results showed that both invertase and the phytoenzymes from A.comosus waste give stretching from 1640.60 cm-1 and 1649.99cm-1 respectively and it is N-H bond. The chromatographic profile of the reaction product from the phytoenzyme of A.comosus waste revealed the presence of sucrose, fructose, glucose and FOS namely, kestose, and nystose. The production of FOS proceeds by fractional factorial design (FFD) central composite design (CCD) statistical methods with the optimisation parameters of reaction time, sucrose concentration, pH, phytoenzyme concentration and temperature using Design Expert version 7.0. All of these responses were screened by FFD method which showed that all the parameters were significant response to produce FOS. CCD method evaluates that pH (6) and temperature 60˚C was producing maximum FOS at 211.334(g/mL). The validation of empirical equations gained from the optimisation study was performed in laboratory scale with only 0.828 % error. As a conclusion, this study has successfully produced FOS using a new approach of alternative source which is from the phytoenzymes of A.Comosus waste.
format	Thesis
qualification_level	Master's degree
author	Nur Dini, Mat Jusoh
author_facet	Nur Dini, Mat Jusoh
author_sort	Nur Dini, Mat Jusoh
title	Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste
title_short	Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste
title_full	Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste
title_fullStr	Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste
title_full_unstemmed	Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste
title_sort	production of fructooligosaccharides (fos) by enzymatic reaction from phytoenzyme of ananas comosus waste
granting_institution	Universiti Malaysia Pahang
granting_department	Faculty of Chemical and Process Engineering Technology
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/31171/1/Production%20of%20fructooligosaccharides%20%28FOS%29%20by%20enzymatic%20reaction%20from%20phytoenzyme.pdf
_version_	1783732158914887680

Production of fructooligosaccharides (FOS) by enzymatic reaction from phytoenzyme of ananas comosus waste

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