Detection and Molecular Characterization of Bacillus cereus Isolated from Sago Processing Plants in Sarawak
Sago processing industries are well-established and high potential industries in Sarawak. However, the contamination of bacteria might deteriorate its quality and become a concern to the public health. This study aimed to detect, quantify and characterize Bacillus cereus in sago processing in Sar...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/30845/1/Jasmin%20binti%20Jaraee.pdf http://ir.unimas.my/id/eprint/30845/4/Jasmin.pdf |
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| Summary: | Sago processing industries are well-established and high potential industries in Sarawak.
However, the contamination of bacteria might deteriorate its quality and become a concern to
the public health. This study aimed to detect, quantify and characterize Bacillus cereus in sago
processing in Sarawak, Malaysia. B. cereus was isolated from two selected sago processing
mills in Sarawak. The prevalence and concentration of B. cereus in this study were determined
firstly using selective agar and followed by using specific Polymerase Chain Reaction (PCR)
by targeting specific virulence gene, haemolysin (hly) gene. A total of 120 samples consist of
bark swab, sago pith, starch slurry, sago milk, sago flour and sago effluent were collected from
each processing step in sago mills. It was revealed that B. cereus were present in 35% (42/120)
of the samples. These isolates were subjected to molecular typing by using Enterobacterial
Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR) and Pulsed Field Gel
Electrophoresis (PFGE). Both molecular typing method showed heterogeneity of the B. cereus
strain. Susceptibility of all isolates towards 14 antibiotics was assessed using disk diffusion
assay. B. cereus isolates were uniformly resistant to penicillin and ampicillin whereas B. cereus
isolates were uniformly susceptible to imipenem and norfloxacin. Multiple antibiotic resistance
(MAR) index were calculated based on the antibiotic resistance results. The MAR index for all
isolates were also varies, ranged from 0.083 to 0.750. This study is useful in developing
appropriate intervention strategies and establishing food safety standards in sago processing in
Sarawak thus contribute in lowering the disease burden and assist in providing safer food to the
society. |
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